We narrowed to 13,278 results for: cDNA

1. pcDNA5/FRT/TO_LAMP1_STf

   Plasmid

   #182006

   Purpose

   CMV overexpression of SNAP-tag fast in the lysosome of mammalian cell lines

   Depositor

   Kai Johnsson

   Article

   Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

   Insert

   LAMP1_STf
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Fast mutation on SNAP-tag E30R

   Promoter

   CMV

   Available since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-MAVS(KaganF57)

   Plasmid

   #52000

   Purpose

   Expresses the human MAVS CDS containing "weak translational context"

   Depositor

   Jonathan Kagan

   Article

   Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

   Insert

   MAVS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 24, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA3.1-MS2-HA-HsMex3C_AD

   Plasmid

   #148481

   Purpose

   Mammalian Expression of HsMex3C

   Depositor

   Elisa Izaurralde

   Insert

   HsMex3C (MEX3C Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   L11P in Mex3C

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-Rs7327-RBD-8h

   Plasmid

   #161823

   Purpose

   Mammalian expression plasmid for RBD of bat coronavirus isolate Rs7327

   Depositor

   Erik Procko

   Article

   Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf

   Insert

   Receptor-binding domain (RBD) of spike protein S
   

   Use

   Tags

   GSG linker and 8his tag and HA leader sequence

   Expression

   Mammalian

   Mutation

   Human codon optimized RBD (a.a. T321-D519)

   Promoter

   CMV

   Available since

   Nov. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDNA4.TO-ORF74-2xNSTREP

   Plasmid

   #136233

   Purpose

   Expresses N-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF74

   Depositor

   Britt Glaunsinger

   Article

   Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

   Insert

   ORF74
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-optoB2-SS-YFP

   Plasmid

   #75270

   Purpose

   Arrestin independent version of optoB2 with serine mutations at S362A,S363G

   Depositor

   Michael Bruchas

   Article

   Siuda et al Nat Commun. 2015 Sep 28;6:8480. doi: 10.

   Insert

   Arrestin independent version of optoB2
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   serine mutations at S362A,S363G

   Promoter

   Available since

   Oct. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-sACE2v2.4-IgG1

   Plasmid

   #149668

   Purpose

   Mammalian expression plasmid for soluble ACE2-Fc fusion (IgG1) high affinity 2.4

   Depositor

   Erik Procko

   Article

   Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

   Insert

   Angiotensin-converting enzyme 2 (ACE2 Human)
   

   Use

   Tags

   Fc region of human IgG1

   Expression

   Mammalian

   Mutation

   Extracellular, soluble protease domain (a.a. M1-D…

   Promoter

   CMV

   Available since

   May 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDNA3-V5-PTPN14-PPxY

   Plasmid

   #61006

   Purpose

   Expresses human PTPN14 (PPxY domain mutant) in mammalian cells, N-terminus V5 tag

   Depositor

   Jianmin Zhang

   Article

   Wilson et al J Biol Chem. 2014 Aug 22;289(34):23693-7

   Insert

   PTPN14 PPxY mutation (PTPN14 Human)
   

   Use

   Tags

   V5 tag

   Expression

   Mammalian

   Mutation

   PPxY mutation

   Promoter

   CMV

   Available since

   Dec. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA3.1 FLIPE-100u

   Plasmid

   #13543

   Depositor

   Wolf Frommer

   Article

   Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

   Insert

   FLIP-E S118W
   

   Use

   Tags

   ECFP and Venus

   Expression

   Mammalian

   Mutation

   S118W in binding protein

   Promoter

   Available since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1 (-) hYAP 1-1delta

    Plasmid

    #124142

    Purpose

    Protein expression for funtional studies of cell growth promotion

    Depositor

    Marius Sudol, Megan Finch-Edmondson

    Article

    Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    None (Wild Type)

    Promoter

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA4/TO/Strep-HA-ZAKalpha_deltaSdeltaCTD

    Plasmid

    #141198

    Purpose

    Expresses Strep-HA-ZAKalpha_deltaSdeltaCTD in mammalian cells, can be used to make inducible cell lines

    Depositor

    Simon Bekker-Jensen

    Article

    Vind et al Mol Cell. 2020 Apr 9. pii: S1097-2765(20

    Insert

    MAP3K20 (MAP3K20 Human)
    

    Use

    Tags

    Strep, HA

    Expression

    Mammalian

    Mutation

    deletion of last 27 amino acids and amino acids 6…

    Promoter

    CMV

    Available since

    June 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1(+)-sapphireAKAR-NLS

    Plasmid

    #118467

    Purpose

    cp-T-sapphire-based single-color PKA biosensor targeted to the nucleus.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    sapphireAKAR-NLS
    

    Use

    Tags

    Nuclear localization signal (NLS)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1his p66shc S36A

    Plasmid

    #10973

    Depositor

    Toren Finkel

    Article

    Nemoto et al Science. 2002 Mar 29. 295(5564):2450-2.

    Insert

    p66shc (SHC1 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    S36A

    Promoter

    Available since

    Feb. 14, 2006

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA5-FRT-TO-FH-Nsp13

    Plasmid

    #157713

    Purpose

    mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp13 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    FH-Nsp13 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    Flag-His8

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pcDNA3.1(+)Gαi2-91-NLuc

    Plasmid

    #207149

    Purpose

    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAI2 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

    Depositor

    Dominique Schols, Tom Van Loy

    Article

    Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

    Insert

    GNAI2 (GNAI2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NLuc inserted at position L(91)Q

    Promoter

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA5/FRT/TO-FNTB-HA

    Plasmid

    #86869

    Purpose

    To express FNTB with a HA tag at C terminus.

    Depositor

    Yihong Ye

    Article

    Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

    Insert

    FNTB (FNTB Human)
    

    Use

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-sACE2v2-sfGFP

    Plasmid

    #145173

    Purpose

    Mammalian expression plasmid for sACE2-sfGFP high affinity variant 2

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Use

    Tags

    Linker GSGGSGSGG and superfolder GFP

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease domain (a.a. M1-D…

    Promoter

    CMV

    Available since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA-Pom121 mApple

    Plasmid

    #187674

    Purpose

    In vivo visualization of NPC protein Pom121

    Depositor

    Mary Beckerle

    Insert

    Pom121 (Pom121 Mouse)
    

    Use

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-dCas9-CibN

    Plasmid

    #60552

    Purpose

    Expresses dCas9-CibN in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

    Insert

    dCas9-CibN
    

    Use

    CRISPR

    Tags

    FLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    inactivating Cas9 mutations D10A and H840A

    Promoter

    CMV

    Available since

    Jan. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDNA5/mCherry-pBirA-HA

    Plasmid

    #154458

    Purpose

    expresses mCherry-pBirA-HA/biochemical assay

    Depositor

    Hyun-Woo Rhee

    Article

    Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

    Insert

    mCherry-pBirA-HA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-F, T7 promoter

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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