We narrowed to 7,508 results for: ef1a

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pKS7107

Plasmid

#89051

Purpose

Expresses Nm crRNA, Nm tracrRNA and human codon-optimized NmCas9

Depositor

Ervin Welker

Article

Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

Inserts

Nm crRNA
Nm tracrRNA
hNmCas9

Use

CRISPR

Tags

HA tag, NLS, and SV40 NLS

Promoter

EF1a and human U6

Available Since

April 14, 2017

Availability

Academic Institutions and Nonprofits only

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pEF1α-M2rtTA-T2A-Puro

Plasmid

#169175

Purpose

piggyBac co-transfection transposon containing M2rtTA promoter linked with Puromycin resistance gene, through T2A with GSG linker, subcloned between ends of two ITRs. Pairs with #169174

Depositor

Igor Slukvin

Article

Jung et al Cell Rep. 2021 Feb 16;34(7):108758. doi:

Insert

M2rtTA-T2A-Puro

Use

Piggybac transposon

Expression

Mammalian

Mutation

See Depositor Comments Below

Promoter

EF1a

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-HyperdLbCas12a-tagBFP-NLS-3xFlag

Plasmid

#236385

Purpose

Overexpression of HyperdLbCas12a in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

Insert

HyperdLbCas12a

Use

CRISPR and Lentiviral

Mutation

D156R, D235R, E292R, D350R

Available Since

Dec. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dLbCas12a-EGFP-NLS-3xFlag

Plasmid

#236364

Purpose

Overexpression of dLbCas12a in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

Insert

dLbCas12a

Use

CRISPR and Lentiviral

Mutation

WT

Available Since

Dec. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-HyperdLbCas12a-EGFP-NLS-3xFlag

Plasmid

#236367

Purpose

Overexpression of HyperdLbCas12a in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

Insert

HyperdLbCas12a

Use

CRISPR and Lentiviral

Mutation

D156R, D235R, E292R, D350R

Available Since

Dec. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dLbCas12aRVRR-EGFP-NLS-3xFlag

Plasmid

#236365

Purpose

Overexpression of dLbCas12aRVRR in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

Insert

dLbCas12aRVRR

Use

CRISPR and Lentiviral

Mutation

G532R, K538V, Y542R, K595R

Available Since

Dec. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pJL163

Plasmid

#243686

Purpose

Stably expresses ALDH18A1 CDS with its endogenous 5'UTR and 3'UTR

Depositor

Jonathan Weissman

Article

Luo et al Cell. 2025 Aug 25:S0092-8674(25)00916-X.

Insert

UTRs and CDS of ALDH18A1 (ALDH18A1 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Nov. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pJL179

Plasmid

#243688

Purpose

Stably expresses SUPV3L1 CDS, flanked with endogenous 5' and 3'UTRs of ALDH18A1

Depositor

Jonathan Weissman

Article

Luo et al Cell. 2025 Aug 25:S0092-8674(25)00916-X.

Insert

UTRs and CDS of SUPV3L1 (SUPV3L1 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-mCherry-Parkin

Plasmid

#237397

Purpose

Lentivirus plasmid for the stable expression of mCherry-Parkin in mammalian cells

Depositor

James Hurley

Article

Rose et al Proc Natl Acad Sci U S A. 2025 Aug 5;122

Insert

PRKN (PRKN Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

EF1A

Available Since

Aug. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-HyperdFnCas12a-EGFP-NLS-3xFlag

Plasmid

#236376

Purpose

Overexpression of HyperdFnCas12a in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

Insert

HyperdFnCas12a

Use

CRISPR and Lentiviral

Mutation

E184R, T279R, D340R, D409R

Available Since

July 15, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dFnCas12a-EGFP-NLS-3xFlag

Plasmid

#236375

Purpose

Overexpression of dFnCas12a in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

Insert

dFnCas12a

Use

CRISPR and Lentiviral

Mutation

WT

Available Since

July 15, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-endAsCas12a-EGFP-NLS-3xFlag

Plasmid

#236363

Purpose

Overexpression of endAsCas12a in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

Insert

endAsCas12a

Use

CRISPR and Lentiviral

Mutation

E174R, S542R, K548R

Available Since

July 15, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-HyperdAsCas12a-EGFP-NLS-3xFlag

Plasmid

#236362

Purpose

Overexpression of HyperdAsCas12a in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

Insert

HyperdAsCas12a

Use

CRISPR and Lentiviral

Mutation

E174R, D256R, E321R, E371R

Available Since

July 15, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dAsCas12a-EGFP-NLS-3xFlag

Plasmid

#236361

Purpose

Overexpression of dAsCas12a in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

Insert

dAsCas12a

Use

CRISPR and Lentiviral

Mutation

WT

Available Since

July 15, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1_-DIO-Varnam1-WPRE

Plasmid

#240054

Purpose

Cre dependent Red fluorescent voltage indicator for mammalian expression

Depositor

Mark Schnitzer

Article

Haziza et al Cell, 188, 1-23, August 7, 2025. doi: 10

Insert

VARNAM1

Use

AAV

Promoter

Ef1a

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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lentiGuideFE-mSG-Puro

Plasmid

#226522

Purpose

Cas9 guide RNA scaffold with the F+E scaffold modification with mStayGold-P2A-Puro

Depositor

Neville Sanjana

Article

Caragine et al Nat Commun. 2025 Feb 13;16(1):1611. doi:

Insert

hU6_SpCas9gRNAF+E_EF1a_mStayGold_P2A_Puro

Use

CRISPR and Lentiviral

Tags

mStayGold

Expression

Mammalian

Promoter

U6,EF1a

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-TET1-dCas9

Plasmid

#235599

Purpose

Expresses Tet1CD-dCas9

Depositor

Ting Zhou

Article

Wu et al Stem Cell Reports. 2025 Apr 25:102499. d

Insert

TET1-dCas9 (TET1 SpCas9 is from Streptococcus pyogenes, Human)

Use

CRISPR and Lentiviral

Tags

3xNLS and TET1 CD

Expression

Mammalian

Promoter

EF1a

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pEF.DEST51-PTPN11

Plasmid

#215523

Purpose

Expression vector with Shp2

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

PTPN11 (PTPN11 Human)

Expression

Mammalian

Promoter

EF1a

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pEF.DEST51-PTPN11-Clover

Plasmid

#215525

Purpose

Expression vector with clover-tagged Shp2

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

PTPN11 (PTPN11 Human)

Tags

Clover

Expression

Mammalian

Promoter

EF1a

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pBK1301-AAV-EFSNC-dCjCas9-NIPP1(143-224)

Plasmid

#223146

Purpose

Expression of truncated NIPP1 with dCjCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

NIPP1 (PPP1R8 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 143-224

Promoter

EF1a

Available Since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

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