We narrowed to 6,235 results for: nls

1. PlmdCas12e KRAB

   Plasmid

   #188506

   Purpose

   Expresses FLAG tagged PlmdCas12e KRAB

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   dCas12e
   

   Use

   CRISPR and Lentiviral

   Tags

   NLS-FLAG-KRAB-NLS

   Expression

   Mammalian

   Mutation

   D659A/E756A/D922A

   Promoter

   EF1a

   Available Since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCMV-CB1R-V2tail-TevN-BLITz1-TetR-VP16

   Plasmid

   #89872

   Purpose

   expresses CB1R-iTango component in mammalian cells

   Depositor

   Hyungbae Kwon

   Article

   Lee et al Nat Methods. 2017 May;14(5):495-503. doi

   Insert

   CB1R-V2tail-TevN-BLITz1-TetR-VP16
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. MSP2266

   Plasmid

   #70705

   Purpose

   Bacterial expression plasmid for SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

   Inserts

   mammalian codon-optimized Staphylococcus aureus Cas9
   SaCas9 sgRNA (+84)
   

   Use

   CRISPR

   Tags

   NLS-3xFLAG

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pPyCAG-NE-EGFP-IRES-Pac

   Plasmid

   #183617

   Purpose

   Mammalian expression vector: CAG promoter driving expression of nuclear envelope-tethered EGFP (NLS-EGFP-EmerinTMD). Confers puromycin resistance.

   Depositor

   Sally Lowell

   Article

   Malaguti et al Dev Cell. 2019 Jun 11. pii: S1534-5807(1

   Insert

   EGFP
   

   Tags

   Human EMD transmembrane domain and NLS

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. RNAPII_CTD27-52_K5S

   Plasmid

   #98679

   Purpose

   Expresses 6xHis-tagged C-terminal domain of RNA polymerase II with K5S

   Depositor

   Nicolas Fawzi

   Article

   Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

   Insert

   RNA polymerase II C-terminal domain (POLR2A Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   K1838S, K1859S, K1866S, K1873S, K1887S

   Available Since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. RNAPII_CTD38-52

   Plasmid

   #98685

   Purpose

   Expresses 6xHis-tagged C-terminal domain of RNA polymerase II

   Depositor

   Nicolas Fawzi

   Article

   Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

   Insert

   RNA polymerase II C-terminal domain (POLR2A Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   heptads 38-52, residues 1853-1970

   Available Since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMGF173

   Plasmid

   #96999

   Purpose

   NLS-GFP-lacZ leu1 insertion vector

   Depositor

   Wesley Sundquist, Adam Frost

   Article

   Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

   Insert

   lacZ
   

   Tags

   NLS-GFP

   Expression

   Yeast

   Promoter

   Pnmt1

   Available Since

   Aug. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. kank2_pJet1.2

   Plasmid

   #121980

   Purpose

   for generating kank2 riboprobes for WISH in zebrafish

   Depositor

   GuangJun Zhang

   Article

   Hensley et al Sci Rep. 2016 Jun 13;6:27816. doi: 10.10

   Insert

   kank2 (LOC571549 Zebrafish)
   

   Use

   Cloning vector

   Promoter

   T7

   Available Since

   Feb. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3-myc-mCE(K294A)

   Plasmid

   #82461

   Purpose

   Expression of myc epitope and catalytic inactive form of murine mRNA Capping Enzyme (K294A) in mammalian cells

   Depositor

   Daniel Schoenberg

   Article

   Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

   Insert

   mCE (K294A) (Rngtt Mouse)
   

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Lysine 294 changed to Alanine

   Promoter

   CMV

   Available Since

   Sept. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCR1277

    Plasmid

    #111101

    Purpose

    Expresses RAD51D_-_33446794.23-P1P2 sgRNA in pCR1068

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Insert

    RAD51D gRNA (RAD51D Human)
    

    Use

    Lentiviral

    Available Since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. RNAPII_CTD27-37

    Plasmid

    #98686

    Purpose

    Expresses 6xHis-tagged C-terminal domain of RNA polymerase II

    Depositor

    Nicolas Fawzi

    Article

    Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

    Insert

    RNA polymerase II C-terminal domain (POLR2A Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    heptads 27-37, residues 1773-1852

    Available Since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3-myc NES- mCE(K294A)

    Plasmid

    #82464

    Purpose

    Expresses myc tag and catalytic inactive form of mRNA capping enzyme with N terminal Nuclear export signal (NES)

    Depositor

    Daniel Schoenberg

    Article

    Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

    Insert

    NES mRNA capping enzyme (Rngtt HIV, Mouse)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    K294A

    Promoter

    CMV

    Available Since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCDNA3.0_nucLAMA-G97 IRES mYFP

    Plasmid

    #130713

    Purpose

    Plasmid encoding nuclear localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and mYFP

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    SNAP-Tag-LAMA-G97-NLS IRES mYFP
    

    Tags

    NLS and SNAP-Tag

    Expression

    Mammalian

    Available Since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCDNA3.0_nucLAMA-F98 IRES mYFP

    Plasmid

    #130712

    Purpose

    Plasmid encoding nuclear localization of LAMA-F98 for GFP as a SNAP-Tag-fusion and mYFP

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    SNAP-Tag-LAMA-F98-NLS IRES mYFP
    

    Tags

    NLS and SNAP-Tag

    Expression

    Mammalian

    Available Since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. kank4_pJet1.2

    Plasmid

    #121982

    Purpose

    for generating kank4 riboprobes for WISH in zebrafish

    Depositor

    GuangJun Zhang

    Article

    Hensley et al Sci Rep. 2016 Jun 13;6:27816. doi: 10.10

    Insert

    kank4 (kank4 Zebrafish)
    

    Use

    Cloning vector

    Promoter

    T7

    Available Since

    Feb. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. RNAPII_CTD43-52

    Plasmid

    #98684

    Purpose

    Expresses 6xHis-tagged C-terminal domain of RNA polymerase II

    Depositor

    Nicolas Fawzi

    Article

    Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

    Insert

    RNA polymerase II C-terminal domain (POLR2A Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    hepatds 43-52, residues 1888-1970

    Available Since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLP110_AAV Scramble Control

    Plasmid

    #239419

    Purpose

    Negative control AAV vector for SaCas9-based CRISPR KO

    Depositor

    Subhojit Roy

    Article

    Parra-Rivas et al STAR Protoc. 2025 Jul 21;6(3):103945. do

    Insert

    Scramble control
    

    Use

    AAV

    Available Since

    Sept. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHS1369

    Plasmid

    #239828

    Purpose

    WT OrufIscB mammalian expression

    Depositor

    Feng Zhang

    Article

    Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038

    Insert

    OrufIscB
    

    Tags

    Nucleoplasmin NLS-3xHA and SV40 NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pWN103

    Plasmid

    #233860

    Purpose

    MoClo-YTK; ConLS/R1 cassette; NS3 systems; pTDH3-LexA-NES-NS3-V1-NLS-VP48-2XOaf1-tADH1

    Depositor

    Jeff Hasty

    Article

    O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

    Insert

    pTDH3-LexA-NES-NS3-V1-NLS-VP48-2XOaf1-tADH1
    

    Expression

    Yeast

    Available Since

    May 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pWN104

    Plasmid

    #233861

    Purpose

    MoClo-YTK; ConLS/R1 cassette; NS3 systems; pTDH3-LexA-NES-NS3-V2-NLS-VP48-2XOaf1-tADH1

    Depositor

    Jeff Hasty

    Article

    O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

    Insert

    pTDH3-LexA-NES-NS3-V2-NLS-VP48-2XOaf1-tADH1
    

    Expression

    Yeast

    Available Since

    May 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge