We narrowed to 16,615 results for: puromycin

1. pEM705 Gs15

   Plasmid

   #109360

   Purpose

   GsX chimera with Gα15 C-terminus

   Depositor

   Robert Lucas

   Article

   Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

   Insert

   Gs15 chimera
   

   Tags

   None

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. p667-UBC-BRSK2-V5-TurboID_IDG-K

   Plasmid

   #135253

   Purpose

   Gateway destination clone of BRSK2 (human) tagged with C-terminal V5-TurboID for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   BRSK2-V5-TurboID (BRSK2 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   V5-TurboID

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   Jan. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDH-2xMARS-nGFP-puro

   Plasmid

   #205238

   Purpose

   Lentiviral vector encoding two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody

   Depositor

   Jeremy Baskin

   Insert

   2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)
   

   Use

   Lentiviral

   Tags

   HA, Nuclear Export Sequence, mScarlet-i, and nGFP…

   Expression

   Mammalian

   Mutation

   amino acids 143-271 of PLEKHA5 (GenBank reference…

   Promoter

   CMV

   Available Since

   July 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMSCVpuro-LssmOrange

   Plasmid

   #96937

   Purpose

   Retroviral plasmid containing LssmOrange for labeling of cells

   Depositor

   Lee Grimes

   Article

   Kleeman et al Cytometry A. 2018 Mar 13. doi: 10.1002/c

   Insert

   Long Stokes Shift Monomeric Orange
   

   Expression

   Mammalian

   Available Since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. p663-UBC-miniTurbo-V5-CSNK1G2_IDG-K

   Plasmid

   #214459

   Purpose

   Gateway destination clone of CSNK1G2 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   miniTurbo-V5-CSNK1G2 (CSNK1G2 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   miniTurbo-V5

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. px459 sgFIP200

   Plasmid

   #175024

   Purpose

   CRISPR-Cas9 plasmid targeting exon 3 of human FIP200.

   Depositor

   David Tumbarello

   Article

   Assar et al Front Cell Dev Biol. 2020 Aug 4;8:733. d

   Insert

   RB1CC1 (RB1CC1 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. N113 nLV EF-1a-Gal4-Stop-PGK-Puro

   Plasmid

   #44176

   Depositor

   Jerry Crabtree

   Article

   Hathaway et al Cell. 2012 Jun 22;149(7):1447-60. doi: 1

   Insert

   Gal4/DBD (1-147)
   

   Use

   Lentiviral

   Promoter

   EF-1a

   Available Since

   June 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. CAG.3xHalo-GFP-SPC25

   Plasmid

   #176206

   Purpose

   Kinetochore anchor for TMP-Halo dimerization system

   Depositor

   Michael Lampson

   Article

   Chen et al J Cell Biol. 2021 Jun 7;220(6). pii: 212

   Insert

   3xHalo-GFP-SPC25
   

   Expression

   Mammalian

   Available Since

   Nov. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. p663-UBC-miniTurbo-V5-DYRK1B_IDG-K

   Plasmid

   #158183

   Purpose

   Gateway destination clone of DYRK1B (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   miniTurbo-V5-DYRK1B (DYRK1B Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   miniTurbo-V5

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   Jan. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. p663-UBC-miniTurbo-V5-CDK18_IDG-K

    Plasmid

    #158180

    Purpose

    Gateway destination clone of CDK18 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-CDK18 (CDK18 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. LPCX Smad3 deltaSSVS

    Plasmid

    #12639

    Depositor

    Rik Derynck

    Article

    Choy et al J Cell Biol. 2000 May 1. 149(3):667-82.

    Insert

    Smad3 (SMAD3 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Last 4 aa's deleted.

    Available Since

    Oct. 10, 2006

    Availability

    Academic Institutions and Nonprofits only

12. LentiGuide-BC-CMV-Puro

    Plasmid

    #127169

    Purpose

    PCR template for CMV-puro resistance cassette

    Depositor

    Paul Blainey

    Article

    Feldman et al Cell. 2019 Oct 17;179(3):787-799.e17. do

    Insert

    None
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSR-puro-Exo84

    Plasmid

    #31116

    Depositor

    Christopher Counter

    Article

    Issaq et al Mol Cancer Res. 2010 Feb . 8(2):223-31.

    Insert

    Exo84 shRNA (EXOC8 Human)
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Available Since

    Sept. 20, 2011

    Availability

    Academic Institutions and Nonprofits only

14. pBRIT HA/FLAG

    Plasmid

    #17519

    Depositor

    Michael Rudnicki

    Article

    McKinnell et al Nat Cell Biol. 2008 Jan . 10(1):77-84.

    Type

    Empty backbone

    Use

    Cre/Lox and Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Available Since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

15. LV TRE-GFP-TMD-DLL1

    Plasmid

    #194936

    Purpose

    LV construct encoding GFP-TMD-DLL1 ligand under dox-inducible TRE promoter and containing a PGK-rtTA-M2-IRES-Puro regulator/resistance marker cassette.

    Depositor

    John Ngo

    Article

    Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

    Insert

    eGFP-(PDGFR)TMD-rDLL1 ICD (WT)
    

    Use

    Lentiviral

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV-H1-SGIPZ_NF1 sh1miR

    Plasmid

    #119206

    Purpose

    knockdown of NF1

    Depositor

    Helen Morrison

    Article

    Cui et al Hum Mutat. 2018 Nov 8. doi: 10.1002/humu

    Insert

    sh1miR targeting NF1 (NF1 Mouse, Rat, Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHSP70B-CP6-pac

    Plasmid

    #165877

    Purpose

    Basic Puromycin resistance cassette in Alpha1, lacks physical marker or attB site

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Basic Selection Vector
    

    Use

    Synthetic Biology

    Available Since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pN-PURO-PTP

    Plasmid

    #51711

    Purpose

    contains an N-terminal tandem affinity purification tag (relevant to any system) within a construct designed for genome integration into trypanosomes.

    Depositor

    Arthur Gunzl

    Article

    Schimanski et al Eukaryot Cell. 2005 Nov;4(11):1942-50.

    Type

    Empty backbone

    Use

    T. brucei genome integration vectors

    Tags

    PTP (ProtC-TEV-ProtA)

    Available Since

    March 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. p667-UBC-AAK1-V5-miniTurbo_IDG-K

    Plasmid

    #135255

    Purpose

    Gateway destination clone of AAK1 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    AAK1-V5-miniTurbo (AAK1 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-miniTurbo

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. aCE120

    Plasmid

    #204491

    Purpose

    Figure 1. reporter circuit

    Depositor

    Ron Weiss

    Article

    Wauford et al Cell Syst. 2023 Sep 20;14(9):806-818.e5.

    Inserts

    EBFP2
    mKate2
    EYFP
    

    Use

    Synthetic Biology

    Tags

    none

    Expression

    Bacterial and Mammalian

    Promoter

    hEF1a

    Available Since

    Dec. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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