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pMRE-Tn7-163

Plasmid

#118575

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

sYFP2

Use

Minitn7 delivery vector

Expression

Bacterial

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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cyto-YFP-FKBPx5

Plasmid

#103777

Purpose

expression of iPOLYMER component in mammalian cells; has nuclear exclusion sequence

Depositor

Takanari Inoue

Article

Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

Insert

cyto-YFP-FKBPx5

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 7, 2018

Availability

Academic Institutions and Nonprofits only

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hsDCX-pHAT

Plasmid

#83928

Purpose

hsDCX-pHAT N-terminal 6xHis-tag, C-terminal Strep-tagII

Depositor

Gary Brouhard

Article

Bechstedt et al Dev Cell. 2012 Jul 17;23(1):181-92. doi:

Insert

human Doublecortin isoform b (DCX Human)

Tags

6-His tag and Strep-TagII

Expression

Bacterial

Available Since

Dec. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-FLEX-RiboL1-jGCaMP8f

Plasmid

#192618

Purpose

AAV transfer plasmid for neuronal expression of Cre-dependent (FLEX), soma-targeted (RiboL1) jGCaMP8f.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

FLEX-RiboL1-jGCaMP8f

Use

AAV and Cre/Lox

Tags

6xHis

Expression

Mammalian

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pLL5.0 E-cadherin shRNA/Ecad-tdTomato

Plasmid

#101278

Purpose

Lentiviral expression of shRNA targeting Ecad and Ecad-tdtomato

Depositor

Alpha Yap

Article

Wu et al Nat Cell Biol. 2014 Feb;16(2):167-78. do

Insert

E-cadherin

Use

Lentiviral

Tags

tdtomato

Expression

Mammalian

Available Since

Jan. 16, 2018

Availability

Academic Institutions and Nonprofits only

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hBACCS2-IRES-mCherry

Plasmid

#72892

Purpose

Mammalian expression of hBACCS2, a human blue light-activated Ca2+ channel switch, and mCherry

Depositor

Takao Nakata

Article

Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

Insert

hBACCS2-IRES-mCherry

Tags

IRES-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 27, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-FLEX-GT

Plasmid

#26198

Depositor

Edward Callaway

Article

Wall et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

Insert

Enhanced green fluorescent protein, TVA (eGFP TVA is a mouse codon optimized version of an avian gene., Mouse)

Use

AAV and Cre/Lox

Mutation

TVA mammalian codon optimized

Available Since

Sept. 16, 2010

Availability

Academic Institutions and Nonprofits only

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pAAV-pCAG eGFP T2A SP HALO HAPLN1

Plasmid

#228200

Purpose

AAV vector expressing GFP and HaloTag-HAPLN1 under constitutive promoter

Depositor

Sungjin Park

Article

Sterin et al J Neurosci. 2024 Sep 9:e0666242024. doi:

Insert

HAPLN1 (Hapln1 Mouse)

Use

AAV

Expression

Mammalian

Promoter

constitutive CAG promoter

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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HP35 tension sensor

Plasmid

#101250

Purpose

Expresses the HP35-based tension sensor module. The biosensor allows force measurements at 6-8 pN.

Depositor

Carsten Grashoff

Article

Austen et al Nat Cell Biol. 2015 Dec;17(12):1597-606.

Insert

YPet(short)-HP35-mCherry

Use

Retroviral

Tags

His-tag and cysteine

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 2, 2017

Availability

Academic Institutions and Nonprofits only

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mActA-FKBP-mCherry

Plasmid

#210295

Purpose

ActuAtor for FKBP-FRB CID

Depositor

Takanari Inoue

Article

Nakamura et al Cell Rep. 2023 Sep 2:113089. doi: 10.101

Insert

mActA-FKBP-mCherry

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pMRE167

Plasmid

#118515

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mClover3

Expression

Bacterial

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-HiBiT-CD9

Plasmid

#162592

Purpose

N-terminally HiBiT-tagged human CD9 under CAG promoter

Depositor

Masaharu Somiya

Article

Somiya et al Anal Chem. 2021 Mar 24. doi: 10.1021/acs

Insert

human CD9 (CD9 Synthetic, human, Human)

Tags

HiBiT

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn7-160

Plasmid

#118572

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

TagBFP2

Use

Minitn7 delivery vector

Expression

Bacterial

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV5-rat ERK2-L73P/S151D

Plasmid

#40819

Depositor

Natalie Ahn

Article

Emrick et al J Biol Chem. 2001 Dec 7;276(49):46469-79

Insert

MAPK1 (Mapk1 Rat)

Tags

His6

Expression

Mammalian

Mutation

L73P, S151D

Promoter

CMV

Available Since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only

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bArr1-V5

Plasmid

#201492

Purpose

Expression of the beta-arrestin1 containing a C-terminal V5 tag.

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

bArr1-V5 (ARRB1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pCin4-3xFLAG-MKL1

Plasmid

#19838

Depositor

Ron Prywes

Article

Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

Insert

MKL1 (MRTFA Human)

Tags

3xFLAG

Expression

Mammalian

Available Since

Dec. 15, 2008

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn7-166

Plasmid

#118578

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mCardinal

Use

Minitn7 delivery vector

Expression

Bacterial

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pS848∙CsR

Plasmid

#199240

Purpose

Expresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteria

Depositor

Susanne Häußler

Article

Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

Insert

gRNA scaffold - Gm resistance cassette - incP oriT

Use

CRISPR and Synthetic Biology

Promoter

PEM7

Available Since

Oct. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1 3xHA-MKK6-EE

Plasmid

#47579

Purpose

Bacterial expression plasmid for GST-3xHA-tagged constitutively active MKK6

Depositor

Kevin Janes

Article

Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

Insert

MKK6-EE (MAP2K6 Human)

Tags

3xHA and GST

Expression

Bacterial

Mutation

Ser207 to Glu, Thr211 to Glu

Available Since

Aug. 28, 2013

Availability

Academic Institutions and Nonprofits only
mCherry-NES-SspB-LARG-DH-P2A-iLIDcaax

Plasmid

#173870

Purpose

Mammalian expression of mCherry-NES-SspB-LARG-DH-P2A-iLIDcaax (opto-RhoA)

Depositor

Takao Nakata

Article

Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

Insert

mCherry-NES-SspB-LARG-DH-P2A-iLIDcaax

Tags

C-terminal CAAX-box and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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