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Gal4 PGC1 alpha 1-350

Plasmid

#8893

Depositor

Bruce Spiegelman

Article

Puigserver et al Science 1999 Nov 12;286(5443):1368-71.

Insert

PGC-1 alpha (Ppargc1a Mouse)

Tags

Gal4 DBD

Expression

Mammalian

Mutation

amino acids 1-350 of PGC-1 alpha

Available Since

Aug. 19, 2005

Availability

Academic Institutions and Nonprofits only
Sema5a-AP-His

Plasmid

#72035

Purpose

Expresses the extracellular region of the Sema5A protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema5a (Sema5a Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 19, 2016

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-PGAM1_sgRNA2

Plasmid

#201615

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

PGAM1 (PGAM1 Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pFlox_N-Mau2_BSD_FKBPF36V_EYFP

Plasmid

#163880

Purpose

Acute Depletion of Mau2-EYFP

Depositor

Cornelis Murre

Article

Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

Insert

Mau2 (Mau2 Mouse)

Expression

Mammalian

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pSG5-ERRbeta-delta10

Plasmid

#52187

Purpose

Expresses human ERRbeta-delta10 splice variant in mammalian cells

Depositor

Rebecca Riggins

Article

Heckler et al Cell Cycle. 2014 Dec 12:0.

Insert

Estrogen-related receptor beta (ESRRB Human)

Expression

Mammalian

Mutation

wild type sequence; codon optimized to exclude Ec…

Promoter

SV40

Available Since

April 24, 2014

Availability

Academic Institutions and Nonprofits only
Sema4b-Fc-His

Plasmid

#72155

Purpose

Expresses the extracellular region of the Sema4B protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema4b (Sema4b Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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Nrp1-AP-His

Plasmid

#71971

Purpose

Expresses the extracellular region of the Neuropilin 1 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Nrp1 (Nrp1 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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Sema4f.b-Fc-His

Plasmid

#72159

Purpose

Expresses the extracellular region of the Sema4F, isoform b protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema4f.b (Sema4f Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pv6_dPC_Chromo_TAF3_Phd

Plasmid

#179403

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of dPC_Chromo - TAF3_Phd

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

dPC_Chromo - TAF3_Phd

Tags

AviTag and EGFP

Expression

Mammalian

Promoter

CAGGS

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCI-neo-Myc-hApg12

Plasmid

#22951

Depositor

Noboru Mizushima

Article

Mizushima et al J Biol Chem. 1998 Dec 18. 273(51):33889-

Insert

autophagy related genes 12 (ATG12 Human)

Tags

3xMyc

Expression

Mammalian

Available Since

June 24, 2010

Availability

Academic Institutions and Nonprofits only
SpyCas9 PE2-3HA

Plasmid

#169853

Purpose

Mammalian Expression, SpyCas9 prime editor with 3HA-tag

Depositor

Scot Wolfe

Article

Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

Insert

SpCas9-H840A-M-MLV-3HA

Tags

3XHA tag and bpSV40 NLS

Expression

Mammalian

Promoter

cmv

Available Since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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cmv hTid Short

Plasmid

#13709

Depositor

Karl Munger

Article

Schilling et al Virology. 1998 Jul 20. 247(1):74-85.

Insert

hTid Short (DNAJA3 Human)

Expression

Mammalian

Available Since

March 5, 2007

Availability

Academic Institutions and Nonprofits only
pGEX-PGC1(1-180)

Plasmid

#1028

Depositor

Bruce Spiegelman

Article

Puigserver et al Science 1999 Nov 12;286(5443):1368-71.

Insert

PGC-1 alpha (Ppargc1a Mouse)

Tags

GST

Expression

Bacterial

Mutation

contains only aa1-180 (the EcoRI site)

Available Since

Sept. 26, 2005

Availability

Academic Institutions and Nonprofits only
pET32a-Trx-PP-hNF2

Plasmid

#107148

Purpose

bacterial expression

Depositor

Tina Izard

Article

Chinthalapudi et al Nat Commun. 2018 Apr 6;9(1):1338. doi: 1

Insert

neurofibromin 2 (NF2 Human)

Tags

(His)6-Thioredoxin

Expression

Bacterial

Promoter

T7

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO DNAJA3

Plasmid

#19466

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

DNAJA3 (DNAJA3 Human)

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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eSpCas9(1.1)_No_FLAG_ATP1A1-G7

Plasmid

#173203

Purpose

Expresses the ATP1A1 G7 sgRNA in combination with FLAGless eSpCas9(1.1) to target ATP1A1 intron 17. pX330-like plasmid.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

ATP1A1 G7 sgRNA + FLAGless enhanced specificity Cas9 (1.1)

Use

CRISPR

Expression

Mammalian

Promoter

U6 promoter

Available Since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pBS-AMV-α1L9'S-nAchR

Plasmid

#20684

Depositor

Henry Lester

Article

Labarca et al Nature. 1995 Aug 10. 376(6540):514-6.

Insert

nicotinic acetylcholine receptors (Chrna1 Mouse)

Tags

AMV and pA 50

Expression

Bacterial

Mutation

Leucine at 9' position in M2 region changed …

Available Since

April 3, 2009

Availability

Academic Institutions and Nonprofits only
Plxnb3-Fc-His

Plasmid

#72130

Purpose

Expresses the extracellular region of the PlexinB3 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Plxnb3 (Plxnb3 Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2016

Availability

Academic Institutions and Nonprofits only

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Sema3d(L)-AP-His

Plasmid

#72017

Purpose

Expresses the Sema3D protein (truncated at cleavage site P3; ie, long), C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3d (Sema3d Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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Cntn4.2-AP-His

Plasmid

#71942

Purpose

Expresses the entire Contactin 2, isoform 2 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Cntn4.2 (Cntn4 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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