We narrowed to 7,306 results for: GFP expression plasmids

1. pAAV-MCU-EGFP

   Plasmid

   #181866

   Purpose

   Expresses GFP-tagged mitochondrial calcium uniporter (MCU) under control of the CaMKIIa promoter

   Depositor

   Hilmar Bading

   Article

   Qiu et al Nat Commun. 2013;4:2034. doi: 10.1038/nc

   Insert

   mitochondrial calcium uniporter (Mcu Mouse)
   

   Use

   AAV and Adenoviral

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CaMKIIa

   Available Since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. HR-TERT-SV40-GFP

   Plasmid

   #71397

   Purpose

   Homologues recombination donor plasmid fo the TERT promotor region introducing a SV-40 driven GFP marker in the TERT promotor. Includes 1kb of TERT coding region in right homologues arm.

   Depositor

   Thomas Cech

   Article

   Xi et al Genome Biol. 2015 Nov 10;16(1):231. doi:

   Insert

   TERT (TERT Human)
   

   Tags

   SV-40 GFP insertion in TERT promotor

   Promoter

   Endogenous TERT promotor

   Available Since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. GFP-P2A-CIAR_pLenti_CMV_Puro

   Plasmid

   #86503

   Purpose

   Lenti plasmid for generating CIAR expressing stable cell lines

   Depositor

   Dustin Maly

   Article

   Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

   Insert

   GFP-P2A-CIAR (SOS1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   T968L in SOScat

   Promoter

   CMV

   Available Since

   Feb. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. (p)odr-3::GFP::EGL-4

   Plasmid

   #100897

   Purpose

   GFP and the EGL-4 cDNA driven by the odr-3 promoter, which expresses in the AWA, AWB, and AWC olfactory sensory neurons in C. elegans. unc-54 3'UTR in sequence as well.

   Depositor

   Noelle L'Etoile

   Article

   Lee et al Proc Natl Acad Sci U S A. 2010 Mar 30;10

   Insert

   56-2741: odr-3 promoter
   

   Expression

   Worm

   Promoter

   odr-3

   Available Since

   Sept. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. CD8a-hnRNP-M-EGFP

   Plasmid

   #86054

   Purpose

   A mammalian expression plasmid encoding CD8a luminal and transmembrane domains followed by hnRNP-M (PY nuclear localization signal of hnRNP-M) and C-terminus EGFP.

   Depositor

   Lei Lu

   Article

   Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

   Insert

   CD8a (CD8A Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   CD8a luminal and transmembrane domians along with…

   Promoter

   CMV

   Available Since

   May 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAVsc.U6-sgFah.CMV/CB-EGFP

   Plasmid

   #121508

   Purpose

   Expresses sgRNA targeting mouse Fah intron 7 (sgFah).

   Depositor

   Guangping Gao

   Article

   Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

   Insert

   sgFah
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. deltaVP1 + VP2C-GFP

   Plasmid

   #166676

   Purpose

   Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and GFP tagged with VP2C (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3)

   Depositor

   Claudia Vickers

   Article

   Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

   Inserts

   VP2C-GFP
   Murine polyomavirus deltaVP1 (NLS deletion mutant)
   

   Use

   Synthetic Biology

   Expression

   Yeast

   Promoter

   GAL1 and GAL10

   Available Since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCLXSN(GFP)-HA-Tbkbp1

   Plasmid

   #125177

   Purpose

   Retroviral expression of mouse Tbkbp1

   Depositor

   Shao-Cong Sun

   Article

   Zhu et al Nat Commun. 2018 Jul 18;9(1):2812. doi:

   Insert

   Tbkbp1 (Tbkbp1 Mouse)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. aTY135_mtk02_pCAG-nfGFPtether_P2A_H2B-IRFP_SV40pA

   Plasmid

   #225350

   Purpose

   piggyback backbone for non-fluorescent GFP tether and H2B-IRFP expression

   Depositor

   Wendell Lim

   Article

   Yamada et al Cell. 2024 Dec 11:S0092-8674(24)01323-0.

   Insert

   H2B (H2B Human, Synthetic)
   

   Tags

   miRFP and non-fluorescent GFP (Y67F)-P2A

   Expression

   Mammalian

   Available Since

   Dec. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pORANGE_Tubb3 GFP(Y39TAG) KI

    Plasmid

    #182678

    Purpose

    Expression of spCas9, gRNA targeting the end of Tubb3 gene and donor GFP(Y39TAG). Can be used for amber codon suppression and click chemistry labeling of endogenous beta-3 tubulin in mammalian cells.

    Depositor

    Ivana Nikić-Spiegel

    Article

    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

    Insert

    Tubb3-targeting gRNA and GFP(Y39TAG) donor sequence (Tubb3, Tubb3 Mouse, Rat)
    

    Expression

    Mammalian

    Promoter

    U6 and chicken beta-actin promoter

    Available Since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. wtVP1 + VP2C-GFPdeg

    Plasmid

    #166678

    Purpose

    Yeast integrative plasmid for expressing wild-type Murine polyomavirus VP1 (GAL1 promoter) and destabilised GFP tagged with VP2C (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

    Inserts

    VP2C-GFPdeg
    Wild-type Murine polyomavirus VP1
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Promoter

    GAL1 and GAL10

    Available Since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. deltaVP1 + VP2C-GFPdeg

    Plasmid

    #166677

    Purpose

    Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and destabilised GFP tagged with VP2C (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

    Inserts

    VP2C-GFPdeg
    Murine polyomavirus deltaVP1 (NLS deletion mutant)
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Promoter

    GAL1 and GAL10

    Available Since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. wtVP1 + VP2C-GFP

    Plasmid

    #166674

    Purpose

    Yeast integrative plasmid for expressing wild-type Murine polyomavirus VP1 (GAL1 promoter) and GFP tagged with VP2C (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

    Inserts

    VP2C-GFP
    Wild-type Murine polyomavirus VP1
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Promoter

    GAL1 and GAL10

    Available Since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAVsc.U6-sgIdua.CMV/CB-EGFP

    Plasmid

    #121511

    Purpose

    Expresses sgRNA targeting mouse Idua intron 9 (sgIdua).

    Depositor

    Guangping Gao

    Article

    Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

    Insert

    sgIdua
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAVsc.U6-sgAspa.CMV/CB-EGFP

    Plasmid

    #121510

    Purpose

    Expresses sgRNA targeting mouse Aspa intron 2 (sgAspa).

    Depositor

    Guangping Gao

    Article

    Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

    Insert

    sgAspa
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMSCV-AVITEV-GFP

    Plasmid

    #74052

    Purpose

    retroviral expression plasmid for GFP with N-terminal BirA-biotinylation signal and TEV celavage site

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    AVITEV-GFP
    

    Use

    Retroviral

    Tags

    AVI-TEV-tag N-terminal to the GFP

    Expression

    Mammalian

    Promoter

    pMSCV-LTRs

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. amilGFP chromoprotein

    Plasmid

    #117842

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses amilGFP chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, amilGFP
    

    Use

    Synthetic Biology; Escherichia coli

    Mutation

    BioBrick sites removed

    Available Since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-T7-ABE8e-SpCas9-NRTH-P2A-EGFP (RMD21)

    Plasmid

    #197503

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-NRTH(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-BPNLS-ABE8e-nSpCas9-NRTH-BPNLS-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8e mutations in TadA and nSpCas9-NRTH(D10A/I32…

    Promoter

    CMV and T7

    Available Since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV-T7-ABE8e-SpCas9-NRRH-P2A-EGFP (RMD51)

    Plasmid

    #197502

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-NRRH(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-BPNLS-ABE8e-nSpCas9-NRRH-BPNLS-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8e mutations in TadA and nSpCas9-NRRH(D10A/I32…

    Promoter

    CMV and T7

    Available Since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-T7-ABE8.20m-SpCas9-NRCH-P2A-EGFP (RMD55)

    Plasmid

    #197501

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE(8.20) A-to-G base editor with nSpCas9-NRCH(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-BPNLS-ABE8.20m-nSpCas9-NRCH-BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8.20 mutations in TadA and nSpCas9-NRCH(D10A/I…

    Promoter

    CMV and T7

    Available Since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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