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pRSFDuet1-tglA

Plasmid

#134673

Purpose

Expresses His6-TglA in E. Coli

Depositor

Wilfred van der Donk

Article

Ting et al Science. 2019 Jul 19;365(6450):280-284.

Insert

TglA

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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pET28b-tglF

Plasmid

#134681

Purpose

Expresses His6-TglF in E. Coli

Depositor

Wilfred van der Donk

Article

Ting et al Science. 2019 Jul 19;365(6450):280-284.

Insert

TglF

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

July 14, 2020

Availability

Academic Institutions and Nonprofits only

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pHis8-4b-McDAH

Plasmid

#140129

Purpose

Expresses McDAH with N-terminal 8xHis-tag in BL21 E. coli

Depositor

Jing-Ke Weng

Article

Kim et al Nat Commun. 2020 Apr 20;11(1):1867. doi:

Insert

Dechloroacutumine halogenase from Menispermum canadense

Tags

8x-His, TEV protease site (ENLYFQ)

Expression

Bacterial

Promoter

T7

Available Since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

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pFETCh_MLLT10_iso1

Plasmid

#135729

Purpose

Donor vector for 3' FLAG tag of human MLLT10_iso1

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

MLLT10_iso1 Homology arms (MLLT10 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pFETCh_ZNF264_iso1

Plasmid

#135731

Purpose

Donor vector for 3' FLAG tag of human ZNF264_iso1

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ZNF264_iso1 Homology arms (ZNF264 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pFETCh_ZBTB21

Plasmid

#135727

Purpose

Donor vector for 3' FLAG tag of human ZBTB21

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ZBTB21 Homology arms (ZBTB21 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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PX330_ZNF503_2

Plasmid

#135755

Purpose

Encodes gRNA for 3' target of human ZNF503

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ZNF503 gRNA (ZNF503 Human)

Use

CRISPR

Promoter

U6

Available Since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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PX330_ONECUT2_2

Plasmid

#135760

Purpose

Encodes gRNA for 3' target of human ONECUT2

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ONECUT2 gRNA (ONECUT2 Human)

Use

CRISPR

Promoter

U6

Available Since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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PX330_ONECUT2_1

Plasmid

#135759

Purpose

Encodes gRNA for 3' target of human ONECUT2

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ONECUT2 gRNA (ONECUT2 Human)

Use

CRISPR

Promoter

U6

Available Since

Jan. 27, 2020

Availability

Academic Institutions and Nonprofits only

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PX458_ZBTB21_1

Plasmid

#135741

Purpose

Encodes gRNA for 3' target of human ZBTB21

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ZBTB21 gRNA (ZBTB21 Human)

Use

CRISPR

Promoter

U6

Available Since

Jan. 27, 2020

Availability

Academic Institutions and Nonprofits only

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PX330_MLLT10_iso1_1

Plasmid

#135745

Purpose

Encodes gRNA for 3' target of human MLLT10_iso1

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

MLLT10_iso1 gRNA (MLLT10 Human)

Use

CRISPR

Promoter

U6

Available Since

Jan. 24, 2020

Availability

Academic Institutions and Nonprofits only

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PX458_ZBTB21_2

Plasmid

#135742

Purpose

Encodes gRNA for 3' target of human ZBTB21

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ZBTB21 gRNA (ZBTB21 Human)

Use

CRISPR

Promoter

U6

Available Since

Jan. 24, 2020

Availability

Academic Institutions and Nonprofits only

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PX330_ZNF264_iso1_1

Plasmid

#135748

Purpose

Encodes gRNA for 3' target of human ZNF264_iso1

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ZNF264_iso1 gRNA (ZNF264 Human)

Use

CRISPR

Promoter

U6

Available Since

Jan. 24, 2020

Availability

Academic Institutions and Nonprofits only

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PX330_ZNF503_1

Plasmid

#135754

Purpose

Encodes gRNA for 3' target of human ZNF503

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ZNF503 gRNA (ZNF503 Human)

Use

CRISPR

Promoter

U6

Available Since

Jan. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2+ AMIC

Plasmid

#118420

Purpose

For mammalian expression of Amoeba myosin 1C

Depositor

Michael Tsang

Article

Saydmohammed et al Nat Commun. 2018 Aug 23;9(1):3381. doi:

Insert

Amoeba myosin 1C

Expression

Mammalian

Mutation

See depositor comments below

Available Since

Sept. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pFETCh_ZNF792

Plasmid

#86282

Purpose

Donor vector for 3' FLAG tag of human ZNF792

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ZNF792 homology arms (ZNF792 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pFETCh_SP5

Plasmid

#86295

Purpose

Donor vector for 3' FLAG tag of human SP5

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

SP5 homology arms (SP5 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pFETCh_ZFP1_iso1

Plasmid

#86294

Purpose

Donor vector for 3' FLAG tag of human ZFP1_iso1

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ZFP1_iso1 homology arms (ZFP1 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_ZNF792_2

Plasmid

#86340

Purpose

Encodes gRNA for 3' target of human ZNF792

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against ZNF792 (ZNF792 Human)

Use

CRISPR

Available Since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_ZSCAN9_1

Plasmid

#86312

Purpose

Encodes gRNA for 3' target of human ZSCAN9

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against ZSCAN9 (ZNF193 Human)

Use

CRISPR

Available Since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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