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p2T-CMV-BE4maxCP1028-BlastR

Plasmid

#152993

Purpose

C-to-T base editor

Depositor

David Liu

Article

Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

Insert

BE4-nSpCas9 CP1028-UGI-UGI

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_AAVS1B

Plasmid

#183338

Purpose

All-in-One CRISPRko system with a guide RNA that targets the AAVS1 locus

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

AAVS1B

Use

Lentiviral

Available Since

May 27, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_FGFR3

Plasmid

#183291

Purpose

All-in-One CRISPRko system with a guide RNA that targets FGFR3 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

FGFR3

Use

Lentiviral

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pET23a-His-TEV-HaloTag-linker-SpyCatcher3

Plasmid

#235150

Purpose

Bacterial expression of His-tagged HaloTag-linker-SpyCatcher3 separated by a TEV protease recognition site.

Depositor

Giordano Lippi

Article

Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

Insert

HaloTag linked to SpyCatcher3

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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mEos4b-Lifeact-7

Plasmid

#54813

Purpose

Localization: Actin, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson, Loren Looger

Article

Paez-Segala et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

Lifeact-mEos4b

Expression

Mammalian

Available Since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pAWP78-PVCpnf_pvc13-antiMouseMHCIINb_Δpvc10

Plasmid

#198450

Purpose

Produces PVCs retargeted with VHH7 anti-mouse MHC class II nanobody; also contains truncated spike tip protein (Pvc10).

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_pvc13-antiMouseMHCIINb_Δpvc10

Expression

Bacterial

Available Since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_TOP2B

Plasmid

#183325

Purpose

All-in-One CRISPRko system with a guide RNA that targets TOP2B gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

TOP2B

Use

Lentiviral

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_TOP2A

Plasmid

#183324

Purpose

All-in-One CRISPRko system with a guide RNA that targets TOP2A gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

TOP2A

Use

Lentiviral

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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E47 2/5-pCS2

Plasmid

#20914

Depositor

Stephen Tapscott

Article

Yang et al Genes Dev. 2009 Mar 15. 23(6):694-707.

Insert

E47 2/5 (TCF3 Human)

Expression

Mammalian

Available Since

April 28, 2009

Availability

Academic Institutions and Nonprofits only
p2T-CMV-CDAmax-BlastR

Plasmid

#152995

Purpose

C-to-T base editor

Depositor

David Liu

Article

Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

Insert

CDA-nSpCas9-UGI-UGI

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-eA3Amax-BlastR

Plasmid

#152997

Purpose

C-to-T base editor

Depositor

David Liu

Article

Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

Insert

eA3A-nSpCas9-UGI-UGI

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

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DT044A

Plasmid

#159405

Purpose

Inducible Perfringolysin O (PFO) expression and purification, controlled under hybrid IPTG-regulated T7/LacO promoter (pRT30)

Depositor

Sheref Mansy

Article

Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

Insert

pr.T7-LacO-6xHis-PFO (Pfo, Clostridium perfringens)

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Promoter

T7/LacO promoter

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pRS-puro shNEDD4L #1

Plasmid

#27016

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

shRNA NEDD4L #1 (NEDD4L Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only

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NLS-tdPCP-GFP

Plasmid

#183934

Purpose

PCP tandem dimer binding to PP7 RNA stem loops; labeled with GFP

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

tdPCP-GFP

Tags

NLS

Expression

Mammalian

Mutation

none

Promoter

CMV (TATA box removed)

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pIE2-DBD

Plasmid

#21602

Depositor

Takahiro Kusakabe

Article

Mon et al Anal Biochem. 2009 Sep 15;392(2):180-2.

Insert

GAL4 DBD (GAL4 Budding Yeast)

Use

Gateway destination vector

Tags

3xHA and Gateway destination cassette

Expression

Insect

Available Since

March 9, 2010

Availability

Academic Institutions and Nonprofits only

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EW410 IKZF1tar x8-H2B-GFP

Plasmid

#236156

Purpose

Fuorescent reporter encoding H2B-GFP fusion under control of E1b minimal promoter with eight IKZF1 binding sites upstream

Depositor

Xiaojing Gao

Article

Wolfsberg et al bioRxiv 2025.02.24.639918

Insert

H2B (H2BC11 Human)

Tags

EGFP

Expression

Mammalian

Promoter

E1b minimal promoter with eight IKZF1 binding sit…

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pNDGG050

Plasmid

#231319

Purpose

BEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid with stability. L1-RK2-Gm-par-ELT4; GG assembly from pOGG004, pOGG009, pOGG010, pOGG012, and pOGG014, GmR

Depositor

Barney Geddes

Article

Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

Type

Empty backbone

Expression

Bacterial

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-rTH-tTA

Plasmid

#133268

Purpose

An AAV genome that expresses tTA from the rat TH promoter

Depositor

Viviana Gradinaru

Article

Robinson et al Elife. 2019 Sep 23;8. pii: 48983. doi: 1

Insert

tTA

Use

AAV

Expression

Mammalian

Mutation

*(see below)

Promoter

rTH

Available Since

Nov. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-PM

Plasmid

#197386

Purpose

To constitutively express Pdx1-Mafa and in cells (Blasticidin resistant).

Depositor

Qiao Zhou

Article

Huang et al Nat Cell Biol. 2023 Apr 27. doi: 10.1038

Insert

PDX1-T2A-MAFA

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pMS12: pDonor(AvCAST)

Plasmid

#168145

Purpose

AvCAST donor harboring non-functional cargo.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Inserts

AvCAST right end (RE)
AvCAST left end (LE)

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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