Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 14,357 results for: cas9

Clear keywords
Showing: 2821 - 2840 of 14357 results

  2. ACE Reporter (pLenti-CMV-mCherry (+43) -T2A-eGFP)

    Plasmid
    #109428
    Purpose
    A lentiviral backbone expressing mCherry with a 43 basepair insert and eGFP off of a CMV promoter. A reporter for both Cas9 nuclease and APOBEC-mediated base-editing activity.
    Depositor
    Reuben Harris
    Publication
    St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994
    Insert
    mCherry T2A GFP
    Use
    Lentiviral
    Mutation
    Inserted 43 base pairs into mCherry to create rep…
    Promoter
    CMV
    Available since
    July 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    109428-plasmid-map-sequence-id-212894

    Enlarge

  3. SGyA

    Plasmid
    #160292
    Purpose
    Drives expression of Cas9 and a TdTomato fluorescent marker
    Depositor
    Omar Akbari
    Publication
    Gamez et al Nat Commun. 2021 Dec 10;12(1):7202. doi:
    Inserts
    Vas promoter
    hSpCas9
    tdTomato
    CTCF insulator
    Gypsy insulator
    Left homology arm Y chromosome
    Right homology arm Y chromosome
    Tags
    T2A GFP
    Expression
    Insect
    Available since
    March 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    160292-plasmid-map-sequence-id-316542

    Enlarge

  4. pUC19-E3HCA8

    Plasmid
    #103100
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Publication
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    E3HCA8(Cas9 coding gene from Ilyobacter polytropus (strain DSM 2926 / CuHBu1))
    Use
    CRISPR; Cloning vector
    Mutation
    human codon-optimized
    Available since
    April 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    103100-plasmid-map-sequence-id-209053

    Enlarge

  5. pSLPB3B-AID-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin-T2A-osTIR1

    Plasmid
    #187960
    Purpose
    AID degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance, T2A site and osTIR1 under PGK promoter.
    Depositor
    Edda Schulz
    Publication
    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AID-SpdCas9-tagRFPt-P2A-tagBFP; Blasticidin-T2A-osTIR1
    Use
    CRISPR and Synthetic Biology
    Tags
    GGS linker
    Expression
    Mammalian
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187960-plasmid-map-sequence-id-373524

    Enlarge

  6. AICSDP-34: TNNI1-mEGFP

    Plasmid
    #114411
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human TNNI1, via excisable Cas9-excisable hPGK-mCherry selection cassette
    Depositor
    Allen Institute for Cell Science
    Publication
    Roberts et al Stem Cell Reports. 2019 May 14;12(5):114
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TNNI1 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (TNNI1 Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Available since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114411-plasmid-map-sequence-id-220877

    Enlarge

  7. pJH2971

    Plasmid
    #100955
    Purpose
    Cas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. KANMX marker
    Depositor
    James Haber
    Publication
    Ranjith et al Protocol Exchange (2017)
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Expression
    Yeast
    Available since
    Sept. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    100955-plasmid-map-sequence-id-193248

    Enlarge

  8. pWT019b

    Plasmid
    #107894
    Purpose
    W1.2 plasmid. Contains PTetO-sd2U-Cas9, PLacO-sgRNA1, PRha-sgRNA2 (sgRNA1-G19T-G20C) and is part of CAMERA 1.2
    Depositor
    David Liu
    Publication
    Tang et al Science. 2018 Feb 15. pii: science.aap89
    Insert
    PTetO-sd2U-Cas9, PLacO-sgRNA1, PRha-sgRNA2 (sgRNA1-G19T-G20C)
    Expression
    Bacterial
    Available since
    April 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    107894-plasmid-map-sequence-id-208429

    Enlarge

  9. pSH-Csy4-T2A-SpRFN-P2A-GFP-multi-gRNA

    Plasmid
    #85756
    Purpose
    All in one plasmid that expresses Csy4, S.pyogenes FokI-dCas9-NLS, and GFP. Also encodes for multiplexed gRNAs. Backbone derived from pSQT1601 (Addgene #53369).
    Depositor
    Lawrence Stanton
    Publication
    Havlicek et al Mol Ther. 2017 Feb 1;25(2):342-355. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Csy4-T2A-SpRFN-P2A-GFP
    gRNA
    Use
    CRISPR
    Tags
    Csy4, Csy4 recognition sequence, FokI fusion via …
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    CMV and U6
    Available since
    Jan. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    85756-plasmid-map-sequence-id-170275

    Enlarge

  12. pAAV-EFS-SpCas9 (AAV8)

    Viral prep
    #104588-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-EFS-SpCas9 (#104588). In addition to the viral particles, you will also receive purified pAAV-EFS-SpCas9 plasmid DNA. EFS-driven expression of Myc-tagged SpCas9. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Ryohei Yasuda
    Publication
    Nishiyama et al Neuron. 2017 Oct 18. pii: S0896-6273(17)
    Promoter
    EFS
    Available since
    Jan. 14, 2026
    Availability
    Academic Institutions and Nonprofits only

  13. pRGEB32-BAR

    Plasmid
    #126072
    Purpose
    For CRISPR/Cas9 delivery in maize. Expresses Cas9 with rice ubiquitin promoter, BAR with 35S promoter, and sgRNA/PTG with rice snoRNA U3 promoter (pol III). Can produce 1 or more gRNAs.
    Depositor
    Charles Hunter
    Publication
    Hunter et al Plants (Basel). 2021 Apr 8;10(4). pii: p
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Plant
    Promoter
    35S for BAR; Rice UBI for Cas9; Rice snoRNA U3 fo…
    Available since
    June 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    126072-plasmid-map-sequence-id-247528

    Enlarge

  14. MoClo Plant Parts III: Transformation & Genome Engineering Kit

    Plasmid kit
    #1000000236
    Purpose
    Collection of MoClo Level 0 parts for plant transformation, including regulatory elements, coding sequences, basic CRISPR parts, and parts for modular geminiviral replicon assembly.
    Depositor
    Daniel Voytas
    Application
    Cloning and Synthetic Biology
    Vector type
    Plant Expression
    Cloning type
    Golden Gate (MoClo)
    Available since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only

  15. pCSD_2XNLS-SpCas9WT-NLS-3xHA-2XNLS-NmCas9WT-2XNLS

    Plasmid
    #107324
    Purpose
    Expresses SpCas9 fused to NmCas9 in mammalian cells
    Depositor
    Scot Wolfe
    Publication
    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9-NmCas9 fusion
    Use
    CRISPR
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    SpCas9 is fused to NmCas9
    Promoter
    CMV IE94
    Available since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    107324-plasmid-map-sequence-id-204720

    Enlarge

  16. gTBEv5

    Plasmid
    #220621
    Purpose
    vector for encoding an engineered glycosylase-based thymine (T) base editor (gTBEv5) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter
    Depositor
    Huawei Tong
    Publication
    Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    nSpCas9(Nterm)-XTEN-hUNG2-XTEN-Cas9(Cterm)
    Expression
    Mammalian
    Mutation
    hUNG2(del1-88;Y156A,A214T,Q259A,Y284D), SpCas9(D1…
    Promoter
    hU6, EF1a
    Available since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    220621-plasmid-map-sequence-id-436953

    Enlarge

  17. gCBEv3

    Plasmid
    #220618
    Purpose
    vector for encoding an engineered glycosylase-based cytosine (C) base editor (gCBEv3) driven by EF1a promoter, guide RNAs compatible with SpG driven by hU6, mCherry driven by CBh promoter
    Depositor
    Huawei Tong
    Publication
    Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    nSpCas9(Nterm)-XTEN-hUNG2-XTEN-Cas9(Cterm)
    Expression
    Mammalian
    Mutation
    hUNG2(del1-88;N213D,K184A,A214V), SpCas9(D10A)
    Promoter
    hU6, EF1a
    Available since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    220618-plasmid-map-sequence-id-436973

    Enlarge

  18. pU6-Universal.Sth.dCas9

    Plasmid
    #171088
    Purpose
    CRISPR interference. Recipient construct for cloning 20-nt spacers into the sgRNA scaffold compatible with Streptococcus thermophilus dCas9 (CRISPR1 system) via BsaI restriction sites.
    Depositor
    Sebastian Lourido
    Publication
    Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Toxoplasma U6 upstream region – spacer cloning site - sgRNA scaffold (compatible with S. thermophilus Cas9 CRISPR1)
    DHFR-TSc3
    Use
    Toxoplasma gondii expression
    Mutation
    Note: A S245F mutation is present but did not app…
    Promoter
    DHFR-TS (Toxoplasma gondii) and U6 (Toxoplasma go…
    Available since
    Oct. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    171088-plasmid-map-sequence-id-335785

    Enlarge

Showing: 2821 - 2840 of 14357 results