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pAAV.Syn-FLEX.iGABASnFR.F102G.mRuby3

Plasmid

#112170

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Insert

iGABASnFR.F102G

Use

AAV

Tags

mRuby3

Mutation

F102G

Promoter

Synapsin

Available Since

July 16, 2018

Availability

Academic Institutions and Nonprofits only

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ptagBFP-LacI

Plasmid

#103839

Purpose

Flourescent marker for lacO arrays

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

tagBFP-LacI

Expression

Mammalian

Mutation

LacI: C19T (silent, L7L), T264C (silent, A88A), C…

Promoter

SV2

Available Since

Dec. 5, 2017

Availability

Academic Institutions and Nonprofits only

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miR-vector-RFP pCDNA

Plasmid

#97143

Purpose

for expression of miRNA and TagRFP

Depositor

Qing Deng

Article

Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

Type

Empty backbone

Tags

TagRFP

Expression

Mammalian

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pSI-check2-Tab1-3'UTR

Plasmid

#97155

Purpose

For dual luciferase report assay

Depositor

Qing Deng

Article

Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

Insert

Tab1 3'UTR

Expression

Mammalian

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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RSRF-Luc-2mut

Plasmid

#31819

Depositor

Astar Winoto

Article

Woronicz et al Mol Cell Biol. 1995 Nov;15(11):6364-76.

Insert

Mutant MEF2-dependent promoter (-307 to -242 of Nur77/NR4A1)

Use

Luciferase

Expression

Mammalian

Mutation

two CTATATTTAG RSRF sites mutated to CGATATTTCG

Promoter

mutated MEF2-dependent (-307 to -242 of Nur77/NR4…

Available Since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only
pCDNA3.1-Muc2D1D2D3

Plasmid

#155215

Purpose

Expresses human MUC2 residues 21-1259

Depositor

Deborah Fass

Article

Javitt et al Cell. 2020 Oct 29;183(3):717-729.e16. do

Insert

Muc2 D1D2D3 (MUC2 Human)

Tags

6xHistadine

Expression

Mammalian

Available Since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRE3 CTF-FLAG

Plasmid

#217691

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRE3 (ADGRE3 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-338

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRE2 CTF-FLAG

Plasmid

#217690

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRE2 (ADGRE2 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-517

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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pBS NL4-3 envFS eGFP Δnef/U3

Plasmid

#101346

Purpose

Deletion from NL4-3 nt 8911-9022 and 9088-9377. This deletes nef and U3 LTR sequences

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

NL4-3

Use

Lentiviral

Mutation

delta nef/LTR

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynap.iGABASnFR.R205A

Plasmid

#112162

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Insert

iGABASnFR.R205A

Use

AAV

Mutation

R205A (no GABA binding)

Promoter

hSynapsin

Available Since

July 16, 2018

Availability

Academic Institutions and Nonprofits only

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pmCellFree_KA1_ZIKVC

Plasmid

#145339

Purpose

Cell-free/mammalian expression vector for Zika virus capsid protein with N-term eGFP

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV Capsid

Tags

8xHis and eGFP

Expression

Bacterial

Promoter

T7- CMV/CAG

Available Since

April 29, 2023

Availability

Academic Institutions and Nonprofits only

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GST-Cav FL (1-178)

Plasmid

#22445

Depositor

William Sessa

Article

Garcia-Cardena et al J Biol Chem. 1997 Oct 10. 272(41):25437-

Insert

Caveolin1(1-178)

Tags

GST

Expression

Bacterial

Available Since

Dec. 8, 2009

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-miniSOG

Plasmid

#193913

Purpose

Control plasmid that expresses non-targeted miniSOG in mammalian cells

Depositor

Xiao Wang

Article

Sessler et al Sci Adv. 2022 Dec 9;8(49):eade1136. doi:

Insert

miniSOG

Use

AAV

Tags

Flag tag

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynap.iGABASnFR.F102Y.Y137L

Plasmid

#112161

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Insert

iGABASnFR.F102Y.Y137L

Use

AAV

Mutation

F102Y.Y137L

Promoter

hSynapsin

Available Since

July 16, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.iGABASnFR.F102Y.Y137L

Plasmid

#112174

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Insert

iGABASnFR.F102Y.Y137L

Use

AAV

Mutation

F102Y.Y137L

Promoter

GFAP

Available Since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3.0_nucLAMA-F98 IRES EGFP

Plasmid

#136618

Purpose

Plasmid encoding nuclear localization of LAMA-F98 for GFP as a SNAP-Tag-fusion and EGFP

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

SNAP-Tag-LAMA-F98-NLS (IRES EGFP)

Expression

Mammalian

Promoter

CMV

Available Since

April 30, 2020

Availability

Academic Institutions and Nonprofits only

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pKPY197

Plasmid

#62599

Purpose

Encodes Thr412Gly-CePheRS Alpha Subunit

Depositor

David Tirrell

Article

Yuet et al Proc Natl Acad Sci U S A. 2015 Feb 17. p

Insert

Thr412Gly-CePheRS Alpha Subunit

Tags

HIS

Expression

Worm

Mutation

Thr412Gly

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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pLEX_970_puro_DEST_V5_BFP

Plasmid

#45297

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

BFP

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

May 22, 2013

Availability

Academic Institutions and Nonprofits only
pET-51b(+) ChemoG 3.2

Plasmid

#193803

Purpose

Protein production of ChemoG3.2 in bacteria

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoG 3.2

Tags

10xHis

Expression

Bacterial

Mutation

EGFP:A206K, T225R; HT7: L271E

Promoter

T7

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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3'-3xFLAG minimal CMV pCDNA5

Plasmid

#212045

Purpose

LR destination vector for integration into N2a rtTA3 expressing cells with an FRT site in the Rosa26 locus

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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