We narrowed to 48,848 results for: Nes;
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Plasmid#117516PurposeCas9_4 Golden Gate Level 0DepositorInsertBsaI:AATG:Cas9_4:GCTT:BsaI
UseCRISPRTags3xFLAG and NLSExpressionPlantAvailable SinceNov. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
BCJJ358
Plasmid#117505PurposeRPS5a:Cas9:E9 Golden Gate Level 1 Position 2 reverseDepositorInsertBpiI:tagt:RPS5a:Cas9_4:E9:ttgc:BpiI
UseCRISPRExpressionPlantPromoterAtRPS5aAvailable SinceOct. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX2TK E7 DLYC
Plasmid#13672DepositorAvailable SinceMarch 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
6mSPL11F15R14::pBIB-BAS (6mSPL11)
Plasmid#27387DepositorInsertSPL11 mutant (AT1G27360 Mustard Weed)
UseUsed to create transgenic linesMutationmiR156 target sites of SPL11 mutatedAvailable SinceMay 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
6mpSPL10F13R13::pBIB-BAS (6mSPL10)
Plasmid#27408DepositorInsertSPL10 mutant (AT1G27370 Mustard Weed)
UseUsed to create transgenic linesMutationmiR156 target sites of SPL10 mutatedAvailable SinceMarch 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-AMBRA1-3xFLAG
Plasmid#172605PurposeExpresses 3xFLAG-tagged AMBRA1 in mammalian cellsDepositorAvailable SinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBABE-MCS(extended)-Puro (empty vector)
Plasmid#172603PurposeEmpty retroviral vector for the constitutive, near-physiological expression of a gene of interest and a puromycin resistance marker in mammalian cells.DepositorTypeEmpty backboneUseRetroviralExpressionMammalianMutationThe multiple cloning site (MCS) has been edited/e…Available SinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-2xFLAG-2xSTREP-CDK2
Plasmid#172616PurposeExpresses 2xFLAG-2xSTREP-tagged CDK2 in mammalian cellsDepositorAvailable SinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-2xFLAG-2xSTREP-CCND2
Plasmid#172629PurposeExpresses 2xFLAG-2xSTREP-tagged cyclin D2 in mammalian cellsDepositorAvailable SinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-2xFLAG-2xSTREP-CCND1
Plasmid#172648PurposeExpresses 2xFLAG-2xSTREP-tagged cyclin D1 in mammalian cellsDepositorAvailable SinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-2xFLAG-2xSTREP-CDK4
Plasmid#172615PurposeExpresses 2xFLAG-2xSTREP-tagged CDK4 in mammalian cellsDepositorAvailable SinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-2xFLAG-2xSTREP-CCND3
Plasmid#172623PurposeExpresses 2xFLAG-2xSTREP-tagged cyclin D3 in mammalian cellsDepositorAvailable SinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CCND1(T286A)
Plasmid#172631PurposeExpresses untagged cyclin D1(T286A) in mammalian cellsDepositorAvailable SinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDestattB (pCM268)
Plasmid#68313PurposeMultisite Gateway destination vector to create transgenes for phiC31-mediated transgenesis in zebrafishDepositorTypeEmpty backboneUseZebrafish transgenesisAvailable SinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJB06
Plasmid#190480PurposeBase Cas9 expression plasmid for 2-plasmid C. difficile mutagenesis systemDepositorInsertsCas9
XylR
UseE. coli / b. subtilis - c. difficile shuttle vect…PromoterxylR-driven promoterAvailable SinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pXL-BacII-CAG-mCherry
Plasmid#170519PurposePlasmid containing piggyBac ITRs flanking CAG and mCherry (also see doi: 10.7554/eLife.63907 )DepositorInsertCAG-mCherry
UsePiggybac transposonExpressionMammalianPromoterCAGAvailable SinceFeb. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-dCas9-KRAB-gRNA-TRE-blast
Plasmid#201152PurposeLentiviral expression of S. pyogenes dead Cas9 (dCas9/dSpCas9/SpdCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.DepositorInsertsdCas9-KRAB
gRNA-TRE
UseCRISPR and LentiviralTagsHAMutationgRNA sequence: TACGTTCTCTATCACTGATAvailable SinceJuly 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCO605
Plasmid#139738Purposeindividual component of Fungal Bioluminescent Pathway, MoClo level 0 plasmid, AsNPGADepositorInsertNPGA
UseLuciferase; Moclo level 0 componentPromoternoneAvailable SinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCO603
Plasmid#139736Purposeindividual component of Fungal Bioluminescent Pathway, MoClo level 0 plasmid, NnLuzDepositorInsertLuz
UseLuciferase; Moclo level 0 componentPromoternoneAvailable SinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
LBJJ382
Plasmid#117518PurposeAlso known as LBJJ429. PCR template for sgRNA-EF with polyT, 67bp or 192bp terminatorDepositorInsertBpiI:ACTA:pU6-26:sgRNA-EF:t192bp:TTAC:BpiI
UseCRISPRExpressionPlantPromoterAtU6-26Available SinceOct. 17, 2018AvailabilityAcademic Institutions and Nonprofits only