We narrowed to 59,421 results for: cloning

1. PP-CyP5

   Plasmid

   #25121

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   49.m03261:C37453
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Aug. 5, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

2. PP-UBC13 (3E95)

   Plasmid

   #25131

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   PFE1350c:C34878
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Aug. 5, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

3. PP-GNPNAT1

   Plasmid

   #25281

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   Tb11.01.2886:V5-L147
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Aug. 5, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

4. pLKO.1 puro

   Plasmid

   #8453

   Purpose

   3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses puromycin for selection.

   Depositor

   Bob Weinberg

   Article

   Stewart et al RNA 2003 Apr;9(4):493-501.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   July 18, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJRH-1362 scFv entry plasmid

   Plasmid

   #201912

   Purpose

   Entry vector for cloning single chain variable fragments displayed on the human CD8a hinge and transmembrane domain (CAG promoter)

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

   Insert

   Stuffer sequence (drop out for scFv cloning) + CD8 hinge and transmembrane domain
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Aug. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-GFP(1-10)

   Plasmid

   #70219

   Purpose

   Expresses GFP(1-10) in mammalian cells

   Depositor

   Bo Huang

   Article

   Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

   Has Service

   Cloning Grade DNA

   Insert

   GFP(1-10)
   

   Expression

   Mammalian

   Available Since

   March 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. tdTomato-N1

   Plasmid

   #54642

   Purpose

   Localization: N1 Cloning Vector, Excitation: 554, Emission: 581

   Depositor

   Roger Tsien, Michael Davidson, Nathan Shaner

   Article

   Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Tags

   tdTomato

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLKO.1-blast

   Plasmid

   #26655

   Purpose

   3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses blasticidin for selection.

   Depositor

   Keith Mostov

   Article

   Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   Dec. 15, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPBT-peRNA_GG-Puro

   Plasmid

   #173220

   Purpose

   piggyBac transposon containing hU6 promotor and pegRNA cloning site

   Depositor

   Jacob Giehm Mikkelsen

   Article

   Wolff et al Front Genome Ed. 2021 Nov 12;3:786893. d

   Inserts

   pegRNA cloning cassette
   PuroR
   

   Use

   Piggybac

   Expression

   Mammalian

   Promoter

   EF1-a and hU6

   Available Since

   May 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMBP-LbCas12a

    Plasmid

    #113431

    Purpose

    Expresses 10xHis-MBP fused to LbCas12a

    Depositor

    Jennifer Doudna

    Article

    Chen et al Science. 2018 Apr 27;360(6387):436-439.

    Has Service

    Cloning Grade DNA

    Insert

    LbCas12a
    

    Tags

    MBP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. ColorfulCell

    Plasmid

    #62449

    Purpose

    MXS_chaining vector with 6 cassettes

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Inserts

    CMV::GTS-Citrine-bGHpA
    CMV::Lyn-Ceruleanx3-bGHpA
    CMV::NLS-TagBFPx3-bGHpA
    CMV::MTS-AzamiGreen-bGHpA
    CMV::iRFP670-SKL-bGHpA
    CMV::ETS-mCherry-KDEL-bGHpA
    

    Use

    Synthetic Biology

    Available Since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUCC001

    Plasmid

    #124451

    Purpose

    Multi-species CRISPR/Cas9 coexpression system, optimized for Golden Gate cloning of gRNA targets

    Depositor

    John Morrissey

    Article

    Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

    Insert

    HH-BsaI
    

    Expression

    Yeast

    Mutation

    gRNA expression cassette from pUDP002 modified to…

    Promoter

    TDH3 promoter from S.cerevisiae (already present …

    Available Since

    Sept. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCRII_TOPO_hNEAT1

    Plasmid

    #61518

    Purpose

    human NEAT1_v1 short isoform 3.7kb insert in general cloning vector

    Depositor

    Archa Fox

    Article

    Clemson et al Mol Cell. 2009 Mar 27;33(6):717-26. doi:

    Insert

    NEAT1 (NEAT1 Human)
    

    Use

    General cloning vector

    Mutation

    *see comment below

    Available Since

    March 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pC0040-LwaCas13a crRNA backbone

    Plasmid

    #103851

    Purpose

    Backbone for cloning guides that are compatible with LwaCas13a. Contains a 5' direct repeat. Clone using BbsI (BpiI). F overhang AAAC. R overhang AAAA

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Insert

    U6-LwaCas13aDR-BbsI-BbsI-polyT
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. BB2_AB

    Plasmid

    #98534

    Purpose

    empty BB2 for transcription unit cloning

    Depositor

    Diethard Mattanovich, Brigitte Gasser, Michael Sauer

    Article

    Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. Ccas9LacZ

    Plasmid

    #113083

    Purpose

    Enhanced C. elegans Cas9 vector. Ccas9LacZ contains a lacZ coding sequence, two Cas9 and two type-IIs restriction enzyme recognition sites next to the U6 promoter and gRNA sequences.

    Depositor

    Tibor Vellai

    Article

    Sturm et al Nucleic Acids Res. 2018 Jun 19. pii: 504

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Worm

    Available Since

    Sept. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. MSGV Mouse Acceptor

    Plasmid

    #128176

    Purpose

    A TCR Variable Region cloning expression vector using Mouse Constant Regions to reduce mis-pairing with endogenous TCR ref Biotechniques. 2015 Mar 1;58(3):135-9

    Depositor

    David Ott

    Article

    Coren et al Biotechniques. 2015 Mar 1;58(3):135-9. d

    Type

    Empty backbone

    Use

    Retroviral

    Promoter

    MSCV

    Available Since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. p3E-MCS

    Plasmid

    #75174

    Purpose

    Multiple cloning site for insertion of gene of interest for C-terminal fusions.

    Depositor

    Nicholas Cole

    Article

    Don et al Zebrafish. 2016 Sep 15.

    Insert

    Multiple Cloning Site
    

    Use

    Three prime entry vector for multisite gateway th…

    Promoter

    n/a

    Available Since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. STAGR_gRNAScaffold_h7SK

    Plasmid

    #102842

    Purpose

    Can be used as PCR template for a STAgR reaction

    Depositor

    Stefan Stricker

    Article

    Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

    Insert

    gRNAScaffold_h7SKpromoter
    

    Use

    As pcr template

    Promoter

    h7SK

    Available Since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCoofy44

    Plasmid

    #55185

    Purpose

    Insect expression vector for parallel SLIC cloning containing N-terminal 10x His and SumoStar tags. C-terminal tags can be added during SLIC.

    Depositor

    Sabine Suppmann

    Article

    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

    Type

    Empty backbone

    Tags

    10x His, 10x His-Stop-OneStrep-Stop-S-tag-Stop-HP…

    Expression

    Bacterial

    Promoter

    pPH (Polyhedrin promoter)

    Available Since

    July 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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