We narrowed to 10,670 results for: nar;

1. IZ801: pMVP (L3-L2) 6x His epitope tag + WPRE

   Plasmid

   #121761

   Purpose

   pMVP L3-L2 entry plasmid, contains 6x His epitope tag + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest in lentivirus vectors.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   6x His epitope tag + WPRE
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. IH201: pMVP (L5-L4) 3x HA epitope tag

   Plasmid

   #121700

   Purpose

   pMVP L5-L4 entry plasmid, contains 3x HA epitope tag for 4-component MultiSite Gateway Pro assembly. Allows N-term epitope tag fusion to gene of interest.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   3x HA epitope tag (open)
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. KG703: pMVP (L5-L4) 6x His epitope tag

   Plasmid

   #121706

   Purpose

   pMVP L5-L4 entry plasmid, contains 6x His epitope tag for 4-component MultiSite Gateway Pro assembly. Allows N-term epitope tag fusion to gene of interest.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   6x His epitope tag (open)
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMCh1750_S2F-IMCg_doxy-CMV_mChe-Cdc42E7

   Plasmid

   #118614

   Purpose

   expression of mChe-tagged cdc42E7 under doxy-inducible CMV promoter

   Depositor

   Marina Chekulaeva

   Article

   Ciolli Mattioli et al Nucleic Acids Res. 2018 Dec 22. pii: 525

   Insert

   cdc42E7 (Cdc42 Mouse)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   tetON CMV

   Available since

   March 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMCh1809_pBS_cdc42E6-boxB

   Plasmid

   #118609

   Purpose

   cdc42E6 3'UTR-boxB tagged for T3 in vitro transcription to use in pulldown

   Depositor

   Marina Chekulaeva

   Article

   Ciolli Mattioli et al Nucleic Acids Res. 2018 Dec 22. pii: 525

   Insert

   cdc42E6 3'UTR (Cdc42 Mouse)
   

   Use

   In vitro transcription

   Tags

   boxB

   Expression

   Mutation

   Promoter

   T3

   Available since

   March 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMCh1807_pBS_cdc42E7-boxB

   Plasmid

   #118612

   Purpose

   cdc42E7 3'UTR-boxB tagged for T3 in vitro transcription to use in pulldown

   Depositor

   Marina Chekulaeva

   Article

   Ciolli Mattioli et al Nucleic Acids Res. 2018 Dec 22. pii: 525

   Insert

   cdc42E7 3'UTR (Cdc42 Mouse)
   

   Use

   In vitro transcription

   Tags

   boxB

   Expression

   Mutation

   Promoter

   T3

   Available since

   March 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. IO801: pMVP (L5-L4) 3x FLAG epitope tag

   Plasmid

   #121702

   Purpose

   pMVP L5-L4 entry plasmid, contains 3x FLAG epitope tag for 4-component MultiSite Gateway Pro assembly. Allows N-term epitope tag fusion to gene of interest.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   3x FLAG epitope tag (open)
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV CMV-driven AcrIIC1-scaffold (2xBsmBI sites)

   Plasmid

   #120300

   Purpose

   AAV vector for expression of AcrIIC1 (no miR binding sites, control vector)

   Depositor

   Dominik Niopek

   Article

   Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

   Insert

   AcrIIC1
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV CMV-driven AcrIIC1-2xmiR-122 target sites

   Plasmid

   #120302

   Purpose

   AAV vector for expression of AcrIIC1 with two miR-122 binding sites

   Depositor

   Dominik Niopek

   Article

   Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

   Insert

   AcrIIC1-2xmiR-122 binding sites
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV CMV-driven AcrIIC3-2xmiR-122 target sites

    Plasmid

    #120303

    Purpose

    AAV vector for expression of AcrIIC3 with two miR-122 binding sites

    Depositor

    Dominik Niopek

    Article

    Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

    Insert

    AcrIIC3-2xmiR-122 binding sites
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

11. pET28a_6HT-TBP6.7(WT)

    Plasmid

    #112720

    Purpose

    Wild-type TBP6.7 was one of the first evolved TAR-binding proteins to bind with exceptional affinity. It was this protein that was used to obtain co-crystals with WT TAR RNA.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(WT)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a_6HT-TBP6.7(R47A)

    Plasmid

    #112722

    Purpose

    Arg47 within TBP6.7 is the most critical residue in terms of forming interactions with WT TAR. Mutating this amino acid to Ala results in ~600-fold reduction in Kd.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(R47A)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    R47A

    Promoter

    T7

    Available since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a_6HT-TBP6.7(R47K)

    Plasmid

    #112723

    Purpose

    Arg47 of TBP6.7 interacts with Hoogsteen edge of TAR's Gua26. Mutating this amino acid to Lys reduces binding to the WT TAR RNA ~330-fold.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(R47K)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    R47K

    Promoter

    T7

    Available since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a_6HT-TBP6.7(Q48A)

    Plasmid

    #112724

    Purpose

    Gln48 contacts the backbone of TAR RNA as well as mediates intramoecular interaction to stabilize the conformation of beta2-beta3 loop within TBP6.7.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(Q48A)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    Q48A

    Promoter

    T7

    Available since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28a_6HT-TBP6.7(T50A)

    Plasmid

    #112727

    Purpose

    Lav-evolved amino acid T50 within TBPs is important in engaging intramolecularly to steer also lab-evolved R49 into conformation compatible binding to RNA.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(T50A)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    T50A

    Promoter

    T7

    Available since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a_6HT-TBP6.7(R52A)

    Plasmid

    #112729

    Purpose

    52nd position is Arg in both wild-type U1A and TBPs, but the RNA recognition by this residue in both types of proteins is fundamentally different.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(R52A)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    R52A

    Promoter

    T7

    Available since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28a_6HT-TBP6.7(delta C-term)

    Plasmid

    #112731

    Purpose

    This mutant of TBP6.7 is lacking residues 91-98 at C-terminus, and was used to test the involvement of those residues in TAR recognition.

    Depositor

    Joseph Wedekind

    Article

    Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

    Insert

    6His-TEV-TBP6.7(delta C-term)
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    delta C-term

    Promoter

    T7

    Available since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. CRY-GalVP16 (B695)

    Plasmid

    #92031

    Purpose

    Expresses CRY2 (full length, intact NLS) fusion with Gal4DBD(aa1-147) fused to VP16AD (truncated), downstream of mCherry-IRES.

    Depositor

    Chandra Tucker

    Article

    Pathak et al Nucleic Acids Res. 2017 Apr 20. doi: 10.

    Insert

    CRY2 (CRY2 Mustard Weed)
    

    Use

    Tags

    GalBD-VP16AD

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRB117.2

    Plasmid

    #181949

    Purpose

    aTc-inducible expression of NarL with N-terminal mNeonGreen fusion. Also contains mCherry under NarL-controlled promoter PdcuS

    Depositor

    Jeffrey Tabor

    Article

    Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11

    Insert

    mNG-NarL; mCherry; tetR (narL, AX04_RS23485 Synthetic, narL - E. coli)
    

    Use

    Tags

    mNeonGreen

    Expression

    Bacterial

    Mutation

    See Depositor Comments Below

    Promoter

    mNG-NarL-PLtetO-1; mCherry-PdcuS; tetR-J23106

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRB113.2

    Plasmid

    #181948

    Purpose

    aTc-inducible expression of NarL with C-terminal mNeonGreen fusion. Also contains mCherry under NarL-controlled promoter PdcuS

    Depositor

    Jeffrey Tabor

    Article

    Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11

    Insert

    NarL-mNG; mCherry; tetR (AX04_RS23485, narL Synthetic, narL - E. coli)
    

    Use

    Tags

    mNeonGreen

    Expression

    Bacterial

    Mutation

    Promoter

    NarL-mNG-PLtetO-1; mCherry-PdcuS; tetR-J23106

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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