167,093 results

1. pEGFP-rUSP19CYT

   Plasmid

   #155248

   Purpose

   for mammalian overexpression of the CYT isoform of rat USP19 fused to GFP at N-terminus

   Depositor

   Simon Wing

   Insert

   USP19 (Cytoplasmic isoform) (Usp19 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGFP-rUSP19ER

   Plasmid

   #155247

   Purpose

   for mammalian overexpression of the ER isoform of rat USP19 fused to GFP at N-terminus

   Depositor

   Simon Wing

   Insert

   USP19 (ER isoform) (Usp19 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPEE16

   Plasmid

   #128360

   Purpose

   Dominant marker in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site 1, a small gene-free region. Contains the amdS2 counterselectable marker.

   Depositor

   James Fraser

   Article

   Erpf et al Fungal Genet Biol. 2019 Jun 17;131:10324

   Type

   Empty backbone

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   TEF1

   Available Since

   Oct. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pre_IV_CS10_1033_v1

   Plasmid

   #126860

   Purpose

   4x 1033 b long scaffolds divided by Zn-dependent self-cleaving DNAzyme cassettes, from compatibility group 10, made by method IV, yields linear 1033 b long scaffolds after Zn incubation

   Depositor

   Hendrik Dietz

   Article

   Engelhardt et al ACS Nano. 2019 Apr 22. doi: 10.1021/acsn

   Insert

   IV_CS10_1033_v1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. PKNOX1_pCSdest

   Plasmid

   #53899

   Depositor

   Roger Reeves

   Article

   Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

   Insert

   PKNOX1 (PKNOX1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SP6 and CMV

   Available Since

   Sept. 8, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

6. pAAV-CAG-GFP (AAV PHP.eB)

   Viral Prep

   #37825-PHPeB

   Purpose

   Ready-to-use AAV PHP.eB particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA. CAG-driven GFP expression. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Edward Boyden

   Promoter

   CAG

   Tags

   GFP

   Available Since

   May 4, 2018

   Availability

   Academic Institutions and Nonprofits only

7. pHR-UCOE-EF1a-Zim3-dCas9-P2A-BFP

   Plasmid

   #188777

   Purpose

   dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

   Depositor

   Jonathan Weissman, Marco Jost

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   Zim3-dCas9
   

   Use

   Lentiviral

   Tags

   HA-2xNLS and Zim3 KRAB-NLS fusion

   Expression

   Mutation

   Promoter

   EF1a

   Available Since

   Oct. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. murine TCR OTI-2A.pMIG II

   Plasmid

   #52111

   Purpose

   retroviral expression of both TCRalpha and TCRbeta using a single vector

   Depositor

   Dario Vignali

   Article

   Holst et al Nat Protoc. 2006;1(1):406-17.

   Inserts

   T cell receptor alpha chain (Tcra Mouse)
   T cell receptor beta chain (Tcrb Mouse)
   

   Use

   Retroviral

   Tags

   T2A (from Thosea asigna virus)

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR223_JAK2_WT

   Plasmid

   #81756

   Purpose

   Gateway Donor vector containing JAK2 , part of the Target Accelerator Plasmid Collection. *Please note this is not a true WT JAK2 sequence and contains a V617F mutation.

   Depositor

   Jesse Boehm, David Root, William Hahn

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   JAK2 (JAK2 Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   V617F

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG/ATP9A-HA

    Plasmid

    #209213

    Purpose

    Mammalian expression of ATP9A

    Depositor

    Hye-Won Shin

    Article

    Takatsu et al J Biol Chem. 2011 Nov 4;286(44):38159-38

    Insert

    ATP9A (ATP9A Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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