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pCR3-vsv-CenpB 1-166-Sgo1 N61I

Plasmid

#108498

Purpose

expression of vsv-CenpB 1-166-Sgo1 N61I (no PP2A binding)

Depositor

Susanne Lens

Article

Meppelink et al Cell Rep. 2015 Apr 28;11(4):508-15. doi:

Insert

CenpB-Shugoshin 1 (SGO1 Human)

Tags

VSV

Expression

Mammalian

Mutation

CenpB 1-166 fusion before Sgo1, no PP2A binding N…

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pAc5.1A-Dm-miR-13b-1

Plasmid

#22762

Depositor

Elisa Izaurralde

Article

Rehwinkel et al Mol Cell Biol. 2006 Apr . 26(8):2965-75.

Insert

micro RNA 13b-1 (mir-13b-1 Fly, primary transcript)

Use

Drosophila expression

Available Since

Jan. 30, 2013

Availability

Academic Institutions and Nonprofits only

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myc-actin-NES-1

Plasmid

#60618

Purpose

Encodes MYC tagged beta-actin with a mutation in the NES

Depositor

Primal de Lanerolle

Article

Hofmann et al J Cell Biol. 2009 Jul 27;186(2):193-200.

Insert

Beta-actin (ACTB Human)

Tags

MYC

Expression

Bacterial and Mammalian

Mutation

I178A, L180A

Available Since

Nov. 21, 2014

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf67

Plasmid

#12755

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf67

Expression

Mammalian

Available Since

Dec. 6, 2006

Availability

Academic Institutions and Nonprofits only
pFastBac1-ScVip1-186-1107

Plasmid

#238316

Purpose

ScVip1-186-1107 protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-186-1107

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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1pBB3-ScVip1-536-1107-C793A

Plasmid

#238324

Purpose

1ScVip1-536-1107-C793A protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-C793A

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pBB3-ScVip1-186-522

Plasmid

#238311

Purpose

ScVip1-186-522 protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-186-522

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(hMTOR_1k)-PGKpuro2ABFP-W

Plasmid

#208429

Purpose

Lentiviral gRNA plasmid targeting human MTOR gene, co-expression of BFP tag

Depositor

Sok Ching Cheong

Article

Chai et al Heliyon. 2024 Oct 22;10(21):e39677. doi:

Insert

MTOR (MTOR Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

N/A

Promoter

human U6

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(hMTOR_2b)-PGKpuro2ABFP-W

Plasmid

#208430

Purpose

Lentiviral gRNA plasmid targeting human MTOR gene, co-expression of BFP tag

Depositor

Sok Ching Cheong

Article

Chai et al Heliyon. 2024 Oct 22;10(21):e39677. doi:

Insert

MTOR (MTOR Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

N/A

Promoter

human U6

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pBB3-ScVip1-536-1107-H548A

Plasmid

#238319

Purpose

ScVip1-536-1107-H548A protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-H548A

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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pBB3-ScVip1-536-1107

Plasmid

#238312

Purpose

ScVip1-536-1107 protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBB3-ScVip1-536-1107-K554A

Plasmid

#238329

Purpose

ScVip1-536-1107-K554A protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-K554A

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBB3-ScVip1-536-1107-R547A

Plasmid

#238318

Purpose

ScVip1-536-1107-R547A protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-R547A

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBB3-ScVip1-536-1107-R551A

Plasmid

#238320

Purpose

ScVip1-536-1107-R551A protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-R551A

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBB3-ScVip1-536-1107-E991A

Plasmid

#238321

Purpose

ScVip1-536-1107-E991A protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-E991A

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBB3-ScVip1-536-1107-H651A

Plasmid

#238323

Purpose

ScVip1-536-1107-H651A protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-H651A

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBtA3

Plasmid

#238985

Purpose

Mammalian expression of bovine arrestin-3 short isoform

Depositor

Vsevolod Gurevich

Article

Hanson et al J Mol Biol. 2007 Apr 27;368(2):375-87. d

Insert

arrestin-3 short (ARR3 Bovine)

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

May 27, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a-ImpBeta1-mEosEM

Plasmid

#218933

Purpose

Express ImpBeta1 (human) with mEosEM fused at the C-terminus.

Depositor

Siegfried Musser

Article

Sau et al Nature. 2025 Mar 19. doi: 10.1038/s41586

Insert

Importin Beta (KPNB1 Human)

Tags

6xHis and mEos4b (EosEM)

Expression

Bacterial

Promoter

T7

Available Since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-EF1A-mSTING (1-339)

Plasmid

#229740

Purpose

Expresses mouse STING amino acids 1-339 in mammalian cells. Stable expression with piggybac transposase.

Depositor

Shawn Ferguson

Article

Bentley-DeSousa et al J Cell Biol. 2025 Feb 3;224(2):e20231015

Insert

STING (Sting1 Mouse)

Tags

None

Expression

Mammalian

Mutation

Contains amino acids 1-339

Promoter

EF1A

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(H56A, R68A, R97A, K98A)

Plasmid

#231379

Purpose

Express mutant human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cells

Depositor

David Bartel

Article

Mohamed et al Cell Rep. 2025 Jan 28;44(1):115166. doi:

Insert

Human Argonaute2 (AGO2 Synthetic, Human)

Tags

3xFLAG-SUMO_Eu1

Expression

Mammalian

Mutation

H56A, R68A, R97A, K98A

Promoter

CMV

Available Since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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