We narrowed to 15,051 results for: yeast expression

1. ClhN-URA3

   Plasmid

   #207012

   Purpose

   Plasmid backbone for yeast expression, use auxotrophic marker URA3 (Kluyveromyces lactis)

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBBK54 pHomL-RPL18Bp-Neon(gfp)-Link-2xPH(PLCd)-SSA1t-HomR (AmpR)

   Plasmid

   #179038

   Purpose

   Vector containing a yeast integration cassette for RPL18B-driven expression of Neon(gfp)-Link-2xPH(PLCd), a marker for the plasma membrane

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   RPL18Bp-Neon(gfp)-Link-2xPH(PLCd)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   May 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. GFP-MIOX only

   Plasmid

   #166683

   Purpose

   Yeast integrative plasmid for expressing mouse myo-inositol oxygenase fused to GFP (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

   Depositor

   Claudia Vickers

   Article

   Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

   Insert

   GFP-MIOX
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL10

   Available Since

   April 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTEF1-UDH

   Plasmid

   #166679

   Purpose

   Yeast integrative plasmid for expressing P. syringae uronate dehydrogenase, with TEF1 promoter. Contains leucine auxotrophic marker (K. lactis LEU2).

   Depositor

   Claudia Vickers

   Article

   Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

   Insert

   UDH
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1

   Available Since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. wtVP1 only

   Plasmid

   #166673

   Purpose

   Yeast integrative plasmid for expressing wild-type Murine polyomavirus VP1, with GAL1 promoter. Contains uracil auxotrophic marker (K. lactis URA3).

   Depositor

   Claudia Vickers

   Article

   Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

   Insert

   Wild-type Murine polyomavirus VP1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available Since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pFA6a-kanMX6-P3nmt1-mECitrine

   Plasmid

   #105151

   Purpose

   Yeast gene targeting

   Depositor

   Jian-Qiu Wu

   Article

   Ye et al Mol Biol Cell. 2012 Apr;23(7):1181-95. d

   Insert

   kanMX6-P3nmt1-mECitrine
   

   Use

   Tags

   3nmt1-mECitrine

   Expression

   Bacterial and Yeast

   Mutation

   F64L, Q69M, A206K

   Promoter

   3nmt1

   Available Since

   March 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pFA6a-kanMX6-3nmt1-mEOS3.2

   Plasmid

   #105166

   Purpose

   Yeast gene targeting

   Depositor

   Jian-Qiu Wu

   Article

   Liu et al Mol Biol Cell. 2016 Aug 15;27(16):2528-4

   Insert

   kanMX6-3nmt1-mEOS3.2
   

   Use

   Tags

   3nmt1-mEOS3.2

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   3nmt1

   Available Since

   Feb. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRS424-TUB2 - human TUBB1 Cterminal tail

   Plasmid

   #60400

   Purpose

   Expression of chimeric yeast beta tubulin (TUB2) - human beta6 tubulin Cterminal tail (TUBB1) using a GAL promoter

   Depositor

   Ron Vale

   Article

   Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

   Insert

   TUB2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   TUB2 amino acids 429 - end, replaced with human T…

   Promoter

   GAL

   Available Since

   May 6, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pRS424-TUB2 - human TUBB3 Cterminal tail

   Plasmid

   #60397

   Purpose

   Expression of chimeric yeast beta tubulin (TUB2) - human beta3 tubulin Cterminal tail (TUBB3) using a GAL promoter

   Depositor

   Ron Vale

   Article

   Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

   Insert

   TUB2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   C127S; TUB2 amino acids 429 - end, replaced with …

   Promoter

   GAL

   Available Since

   May 6, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pRS424-TUB2 - human TUBB7 Cterminal tail

    Plasmid

    #60399

    Purpose

    Expression of chimeric yeast beta tubulin (TUB2) - human beta7 tubulin Cterminal tail (TUBB7) using a GAL promoter

    Depositor

    Ron Vale

    Article

    Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

    Insert

    TUB2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    TUB2 amino acids 429 - end, replaced with human T…

    Promoter

    GAL

    Available Since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pRS424-TUB2 - human TUBB8 Cterminal tail

    Plasmid

    #60401

    Purpose

    Expression of chimeric yeast beta tubulin (TUB2) - human beta8 tubulin Cterminal tail (TUBB8) using a GAL promoter

    Depositor

    Ron Vale

    Article

    Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

    Insert

    TUB2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    TUB2 amino acids 429 - end, replaced with human T…

    Promoter

    GAL

    Available Since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pRS424-TUB2 - human TUBB4a Cterminal tail

    Plasmid

    #60402

    Purpose

    Expression of chimeric yeast beta tubulin (TUB2) - human beta4a tubulin Cterminal tail (TUBB4a) using a GAL promoter

    Depositor

    Ron Vale

    Article

    Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

    Insert

    TUB2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    TUB2 amino acids 429 - end, replaced with human T…

    Promoter

    GAL

    Available Since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pHCMM3

    Plasmid

    #167454

    Purpose

    A control chimera; expresses C. elegans PUP-2 from the Sec63 promoter without the RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (-PAB)]

    Depositor

    Hugo Cesar Medina-Munoz, Marvin Wickens

    Article

    Medina-Munoz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

    Insert

    PSEC63_yeGFP_cePUP-2_URA3_TADH1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PSEC63

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. SARS-CoV-2 Spike (S) Receptor Binding Domain (RBD) library N501Y Tile 2 (positions 437-527)

    Pooled Library

    #174296

    Purpose

    This library contains mutations to SARS-CoV-2 Spike RBD N501Y variant in which all surface exposed residues on S RBD (Tile 2, positions 437-527) were mutated to every other amino acid.

    Depositor

    Timothy Whitehead

    Article

    Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:

    Expression

    Yeast

    Available Since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

15. SARS-CoV-2 Spike (S) Receptor Binding Domain (RBD) library N501Y Tile 1 (positions 333-436)

    Pooled Library

    #174295

    Purpose

    This library contains mutations to SARS-CoV-2 Spike RBD N501Y variant in which all surface exposed residues on S RBD (Tile 1, positions 333-436) were mutated to every other amino acid.

    Depositor

    Timothy Whitehead

    Article

    Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:

    Expression

    Yeast

    Available Since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

16. SARS-CoV-2 Spike (S) Receptor Binding Domain (RBD) library E484K Tile 2 (positions 437-527)

    Pooled Library

    #174294

    Purpose

    This library contains mutations to SARS-CoV-2 Spike RBD E484K variant in which all surface exposed residues on S RBD (Tile 2, positions 437-527) were mutated to every other amino acid.

    Depositor

    Timothy Whitehead

    Article

    Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:

    Expression

    Yeast

    Available Since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

17. SARS-CoV-2 Spike (S) Receptor Binding Domain (RBD) library E484K Tile 1 (positions 333-436)

    Pooled Library

    #174293

    Purpose

    This library contains mutations to SARS-CoV-2 Spike RBD E484K variant in which all surface exposed residues on S RBD (Tile 1, positions 333-436) were mutated to every other amino acid.

    Depositor

    Timothy Whitehead

    Article

    Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:

    Expression

    Yeast

    Available Since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

18. pRS315e_pGal-nCas9(D10A)-PmCDA1

    Plasmid

    #79617

    Purpose

    Expresses nCas9(D10A)-PmCDA1 in yeast cells

    Depositor

    Akihiko Kondo

    Article

    Nishida et al Science. 2016 Aug 4. pii: aaf8729.

    Insert

    SpCas9
    

    Use

    Tags

    PmCDA1

    Expression

    Yeast

    Mutation

    D10A for nickase Cas9

    Promoter

    pGal1

    Available Since

    July 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFS462

    Plasmid

    #89065

    Purpose

    his7 integration plasmid with Z3EVpr:GFP

    Depositor

    Nick Rhind

    Article

    Ohira et al Yeast. 2017 Aug;34(8):323-334. doi: 10.1

    Insert

    Z3EV promoter GFP-NLS leu1 C-terminal 300 nt
    

    Use

    S. pombe his7 integration vector

    Tags

    Expression

    Mutation

    Promoter

    Z3EV

    Available Since

    July 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDZ645 pKAN 1x-mCherry-12xPP7 V4

    Plasmid

    #73173

    Purpose

    Floxed Kanamycin (G418) selection maker; 3' tagging vector used to add 1x-mCherry followed by 12 repetitive PP7 version 4 RNA stem-loops to gene of interest

    Depositor

    Daniel Zenklusen

    Article

    Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

    Insert

    1x-mCherry
    

    Use

    Tags

    Expression

    Mammalian and Yeast

    Mutation

    Promoter

    Available Since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge