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pPI231

Plasmid

#207432

Purpose

Safe haven knock-in plasmid for the act5 locus for N-terminal mNeonGreen fusions

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Insert

N-terminal mNeonGreen

Use

Unspecified

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pGL2084

Plasmid

#137726

Purpose

Gateway vector for inducible stem loop RNAi in T.b. brucei 2T1

Depositor

Jeremy Mottram

Article

Jones et al PLoS Pathog. 2014 Jan;10(1):e1003886. do

Type

Empty backbone

Use

RNAi; Trypanosoma brucei tet inducible stem loop

Available Since

June 20, 2023

Availability

Academic Institutions and Nonprofits only

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lenti-Cas9-sgHPRT1

Plasmid

#196713

Purpose

CRISPR-KO. WT-SpCas9 and sgRNA targeting HPRT1. Editing-competent cells can be selected with 6-TG

Depositor

Bernhard Schmierer

Article

Schmierer et al Mol Syst Biol. 2017 Oct 9;13(10):945. do

Insert

Cas9-T2A-BSD-U6-sgHPRT1

Use

CRISPR and Lentiviral

Tags

Nucleoplasmin NLS and SV40 NLS

Promoter

EF1a/hU6

Available Since

April 12, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsNanos3_X

Plasmid

#147944

Purpose

Mammalian Expression of HsNanos3

Depositor

Elisa Izaurralde

Article

Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

Insert

HsNanos3 (NANOS3 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsNanos3-F4A_X

Plasmid

#147946

Purpose

Mammalian Expression of HsNanos3-F4A

Depositor

Elisa Izaurralde

Article

Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

Insert

HsNanos3-F4A (NANOS3 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsNanos3-W7E_X

Plasmid

#147947

Purpose

Mammalian Expression of HsNanos3-W7E

Depositor

Elisa Izaurralde

Article

Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

Insert

HsNanos3-W7E (NANOS3 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsNanos3-Y10E_X

Plasmid

#147948

Purpose

Mammalian Expression of HsNanos3-Y10E

Depositor

Elisa Izaurralde

Article

Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

Insert

HsNanos3-Y10E (NANOS3 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pN1-HsSmg5_267-1017-V5-SBP-MBP_V

Plasmid

#147762

Purpose

Mammalian Expression of HsSmg5_267-1017

Depositor

Elisa Izaurralde

Article

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsSmg5_267-1017 (SMG5 Human)

Expression

Mammalian

Mutation

one silent mutation C969T compared to the sequenc…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pN1-HsSmg5-V5-SBP-MBP_V

Plasmid

#147768

Purpose

Mammalian Expression of HsSmg5

Depositor

Elisa Izaurralde

Article

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsSmg5 (SMG5 Human)

Expression

Mammalian

Mutation

one silent mutation C969T compared to the sequenc…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpG-HsNanos3_4-20-Strep_W

Plasmid

#147825

Purpose

Bacterial Expression of HsNanos3_4-20

Depositor

Elisa Izaurralde

Article

Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

Insert

HsNanos3_4-20 (NANOS3 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-HsNanos3_4-20-Strep_W

Plasmid

#147826

Purpose

Bacterial Expression of HsNanos3_4-20

Depositor

Elisa Izaurralde

Article

Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

Insert

HsNanos3_4-20 (NANOS3 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsSmg5-del133-155_T

Plasmid

#147553

Purpose

Mammalian Expression of HsSmg5-del133-155

Depositor

Elisa Izaurralde

Article

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsSmg5-del133-155 (SMG5 Human)

Expression

Mammalian

Mutation

one silent mutation compared to the sequence give…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsSmg7_1-633_T

Plasmid

#147557

Purpose

Mammalian Expression of HsSmg7_1-633

Depositor

Elisa Izaurralde

Article

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsSmg7_1-633 (SMG7 Human)

Expression

Mammalian

Mutation

two silent mutation G216A, A1521G compared to the…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsSmg7_1-633_U

Plasmid

#147644

Purpose

Mammalian Expression of HsSmg7_1-633

Depositor

Elisa Izaurralde

Article

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsSmg7_1-633 (SMG7 Human)

Expression

Mammalian

Mutation

three silent mutation G216A, T1185C and A1521G co…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsNanos3_P

Plasmid

#147200

Purpose

Mammalian Expression of HsNanos3

Depositor

Elisa Izaurralde

Article

Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

Insert

HsNanos3 (NANOS3 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNanos3_P

Plasmid

#147203

Purpose

Mammalian Expression of HsNanos3

Depositor

Elisa Izaurralde

Article

Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

Insert

HsNanos3 (NANOS3 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pSK-mU6-Ccu-sgRNA-SV40-puro

Plasmid

#192130

Purpose

Expresses Ccu-SgRNA scaffold cloned with BbsI and puromycin resistance gene

Depositor

Yongming Wang

Article

Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

Insert

Ccu-SgRNA scaffold

Expression

Mammalian

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pSK-mU6-Hsp1-sgRNA-SV40-puro

Plasmid

#192129

Purpose

Expresses Hsp1-SgRNA scaffold cloned with BbsI and puromycin resistance gene

Depositor

Yongming Wang

Article

Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

Insert

Hsp1-SgRNA scaffold

Expression

Mammalian

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-V5-SBP-C1-HsNanos3_X

Plasmid

#147962

Purpose

Mammalian Expression of HsNanos3

Depositor

Elisa Izaurralde

Article

Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

Insert

HsNanos3 (NANOS3 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1 - TRC mTagBFP2-Synapsin1a

Plasmid

#191567

Purpose

Expresses an shRNA and mTagBFP2-synapsin1a

Depositor

Timothy Ryan

Article

Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

Type

Empty backbone

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6 for shRNA

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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