We narrowed to 9,978 results for: Met;
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Plasmid#61230PurposeLentivirus expressing shRNA to GFPDepositorInsertGFP
UseLentiviralPromoterH1/TOAvailable SinceMarch 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAS_FLAG-Mx1201 PylRS
Plasmid#154764Purposeplasmid with Mx1201 PylRS for amber suppression in transient or stable, piggybac-mediated, integrationDepositorInsertMx1201 PylRS (MMALV_RS05520 Methanomethylophilus alvus Mx1201)
TagsFLAGExpressionMammalianPromoterEF1Available SinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQCXIZ GFP PODXL B WT
Plasmid#202414PurposeExpression of GFP-tagged PODXL (isoform B) WTDepositorAvailable SinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKL11-GCaMP6f bb100
Plasmid#158983PurposeConstitutive expression (sigma 70 promoter) of GCaMP6f fluorophore in E. coli.DepositorInsertGCaMP6f
ExpressionBacterialMutationcodon optimized for e. coli from Addgene#100833PromoterBba_J23100 (sigma 70)Available SinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
VanGlow-RR
Plasmid#83343Purposefor Gateway cloning of promoter elements upstream of a mCherry::Renilla(nls) reporter, facilitating qualitative and quantitative analysis of expression in transgenic fly lines and S2 cells.DepositorTypeEmpty backboneUseLuciferaseTagsmCherry::Renilla(nls)ExpressionInsectAvailable SinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a-hdNadV
Plasmid#83362PurposeExpresses nadV from Haemophilus ducreyi in Escherichia coliDepositorInsertnadV
TagsnoneExpressionBacterialPromoterT7Available SinceOct. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xMx PylT C41CA_FLAG_Mx PylRS
Plasmid#140013PurposePlasmid with 4xMx PylT C41CA cassette and MX1201 PylRS for amber suppression; for transient or stable piggyBac-mediated integrationDepositorInsertMx1201 PylRS (MMALV_RS05520 Methanomethylophilus alvus Mx1201)
TagsFLAGExpressionMammalianPromoterEF1Available SinceMay 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT-ChemoG5
Plasmid#193807PurposeCMV overexpression of ChemoG5 in mammalian cell linesDepositorInsertChemoG5
Tags10xHisExpressionMammalianMutationEGFP:A206K, T225R; HT7: E143R, E147R, L271EPromoterCMVAvailable SinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
EC64422
Plasmid#191768PurposeCRISPR mutagenesis in Medicago truncatulaDepositorInsertSpCas9
UseCRISPRExpressionPlantAvailable SinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSBBi-Pur LaG17-synNotch-TetRVP64
Plasmid#158133PurposeConstitutive expression of anti-GFP nanobody (LaG-17) synNotch TetRVP64 receptor in a pSBBi-Pur Sleeping Beauty backboneDepositorInsertLaG17-synNotch-TetRVP64
UseTransposonExpressionMammalianPromoterEF1αAvailable SinceJune 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
MAC-SARS-CoV-2 Orf9c
Plasmid#158379PurposeMAC-tagged gene expressionDepositorInsertSARS-CoV-2 Orf9c
TagsMAC-tagExpressionMammalianAvailable SinceAug. 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pXWJ109As(RS-te-AAV)
Plasmid#123156PurposeFor TEV-based leakiness regulation. pSB3K3 carrying J109-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-te-AAV-tDepositorInsertJ109-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-te-AAV-t
UseSynthetic BiologyExpressionBacterialPromoterJ109, ParsR, PhrpLEAvailable SinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO/intron/GFP
Plasmid#113547PurposeMammalian expression plasmid that can be used with the Flp-In T-REx system.DepositorInsertGFP
TagsGFPExpressionMammalianAvailable SinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-mEGFP-mTrim21ΔPRYSPRY
Plasmid#105521PurposeT7 promotor drives in vitro transcription of mEGFP-tagged mouse TRIM21 with deleted PRYSPRY domain mRNADepositorInsertTRIM21 (Trim21 Mouse)
UseIn vitro transcriptionTagsmEGFPMutationdeletion of PRYSPRY domain (aa1-281)PromoterT7Available SinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSET a sfGCaMP6s
Plasmid#100022PurposeBacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. cpEGFP replaced with a superfolder cpGFP variant.DepositorInsertsfGCaMP6s
Tags6x HIS tag and Xpress_EK tagExpressionBacterialMutationcpEGFP replaced with superfolder cpGFPPromoterT7Available SinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSuper.Retro.Puro-WDR5 shRNA 2
Plasmid#59975PurposeKnockdown of WDR5DepositorInsertWDR5 shRNA
UseRetroviralAvailable SinceOct. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCLC3sHA
Plasmid#52424Purposemammalian expression of HA tagged rat CLC3 short transcriptDepositorAvailable SinceMay 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBABE-FLAG-hG9a
Plasmid#33023DepositorInserthG9a
UseRetroviralTagsFlagExpressionMammalianPromoter5'LTRAvailable SinceApril 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pR-mKate2
Plasmid#228610PurposeMammalian expression of mKate2, for MuSIC barcode constructionDepositorInsertmKate2
ExpressionMammalianMutationWe introduced some silent mutations to avoid esse…PromoterCMVAvailable SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKT2/CAGXSP/PalmReNL
Plasmid#182970PurposeStably express PalmReNL in mammalian cells via the sleeping beauty transposon system.DepositorInsertPalmReNL
ExpressionMammalianMutationN/AAvailable SinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only