We narrowed to 5,509 results for: SPI

1. Plk1 FRET sensor c-jun substrate

   Plasmid

   #45203

   Depositor

   Michael Lampson

   Article

   Liu et al J Cell Biol. 2012 Aug 20;198(4):491-9. d

   Insert

   Plk1 FRET sensor with c-jun substrate
   

   Use

   Tags

   CyPET, YPET

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available since

   June 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLBH533_MBP-Cas14b2 expression

   Plasmid

   #112506

   Purpose

   Expresses 10xHis-MBP fused to Cas14b2 under t7 promoter

   Depositor

   Jennifer Doudna

   Article

   Harrington et al Science. 2018 Oct 18. pii: science.aav42

   Insert

   Cas14b2
   

   Use

   CRISPR

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. EKARet-nuc

   Plasmid

   #96896

   Purpose

   FLIM sensor for ERK activation in nucleus

   Depositor

   Ryohei Yasuda

   Article

   Tang et al Neuron. 2017 Mar 22;93(6):1315-1324.e3.

   Insert

   FLIM Sensor for ERK Activity in Nucleus
   

   Use

   Tags

   EGFP and sREAChet

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pScalps hygro

   Plasmid

   #184785

   Purpose

   Empty Lentiviral vector backbone

   Depositor

   Ajit Chande

   Article

   Mishra et al Front Cell Infect Microbiol. 2021 Apr 23

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   Sept. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLBH532_MBP-Cas14b1 expression

   Plasmid

   #112505

   Purpose

   Expresses 10xHis-MBP fused to Cas14b1 under t7 promoter

   Depositor

   Jennifer Doudna

   Article

   Harrington et al Science. 2018 Oct 18. pii: science.aav42

   Insert

   Cas14b1
   

   Use

   CRISPR

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET-mKelly1

   Plasmid

   #119782

   Purpose

   Expresses mKelly1 tagged N-terminally with an MHHHHHHGSG tag

   Depositor

   Stephen Mayo

   Article

   Wannier et al Proc Natl Acad Sci U S A. 2018 Nov 27;11

   Insert

   mKelly1
   

   Use

   Tags

   6xHis tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLenti-CamkII-MacQ-mCitrine

   Plasmid

   #48762

   Purpose

   sensor

   Depositor

   Mark Schnitzer

   Article

   Gong et al Nat Commun. 2014 Apr 22;5:3674. doi: 10.

   Insert

   macQ-mCitrine
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   mac-D139Q

   Promoter

   CamKIIa

   Available since

   Jan. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGBW-m4046233

   Plasmid

   #149032

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 nsp3

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 nsp3 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable thrombin;6xHIS

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pTEF1

   Available since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pGBW-m4046664

   Plasmid

   #149224

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 E (envelope)

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 E (envelope) (E Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Saccharomyces cerevisiae recode 1

   Promoter

   pTEF1

   Available since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pTK50_GFP-NuMA-N

    Plasmid

    #46348

    Depositor

    Iain Cheeseman

    Article

    Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

    Insert

    NuMA N-terminal fragment (NUMA1 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    N-terminal fragment from 1-213

    Promoter

    pBABE

    Available since

    July 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTK51_GFP-NuMA-C

    Plasmid

    #46349

    Depositor

    Iain Cheeseman

    Article

    Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

    Insert

    NuMA C-terminal fragment (NUMA1 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    C-terminal fragment from 1700-2115

    Promoter

    pBABE

    Available since

    July 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. N-term Hec1 Plk1 FRET sensor c-jun substrate

    Plasmid

    #45204

    Depositor

    Michael Lampson

    Article

    Liu et al J Cell Biol. 2012 Aug 20;198(4):491-9. d

    Insert

    Hec1-targeted Plk1 FRET sensor
    

    Use

    Tags

    CyPET, YPET

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLenti-CamkII-MacQ-mOrange2

    Plasmid

    #48761

    Purpose

    sensor

    Depositor

    Mark Schnitzer

    Article

    Gong et al Nat Commun. 2014 Apr 22;5:3674. doi: 10.

    Insert

    macQ-mOrange
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    mac-D139Q

    Promoter

    CamKIIa

    Available since

    Jan. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. AKARet-cyto

    Plasmid

    #96897

    Purpose

    FLIM sensor for PKA activation in cytosol

    Depositor

    Ryohei Yasuda

    Article

    Tang et al Neuron. 2017 Mar 22;93(6):1315-1324.e3.

    Insert

    FLIM Sensor for PKA Activity in Cytosol
    

    Use

    Tags

    EGFP and sREAChet

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGBW-m4252984

    Plasmid

    #153898

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 SARS-CoV-2 E (envelope)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 SARS-CoV-2 E (envelope) (E Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    CMV

    Available since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pGBW-m4046479

    Plasmid

    #145495

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 nsp4

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp4 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable thrombin;3xFLAG

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pJMH147

    Plasmid

    #186647

    Purpose

    aadA.co-TaadA-J23107-4ABH.co.rm1-PpsaA-nhoA.co.rm1 between homology arms for pilA

    Depositor

    Adam Arkin

    Article

    Jacob M. et al bioRxiv

    Insert

    aadA.co-TaadA-J23107-4ABH.co.rm1-PpsaA-nhoA.co.rm1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGBW-m4046323

    Plasmid

    #149100

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 nsp3

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp3 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable thrombin;MBP

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pGBW-m4252975

    Plasmid

    #153873

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    CMV

    Available since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pGBW-m4046348

    Plasmid

    #149108

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 nsp6

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp6 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable thrombin;3xFLAG

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge