We narrowed to 8,174 results for: KRAS

1. pTRE3G-gibbon cGAS-HA

   Plasmid

   #186862

   Purpose

   Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

   Insert

   Gibbon cGAS with C-terminal HA tag (CGAS Hylobatidae)
   

   Use

   Retroviral

   Tags

   HA

   Promoter

   TRE3G

   Available Since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. HypaR-SpCas9

   Plasmid

   #126757

   Purpose

   Expression plasmid for human codon-optimized increased fidelity Hypa2SpCas9 (without U6-sgRNA coding sequence, with silent mutations)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   Hypa2SpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   R661A, N692A, M694A, Q695A, H698A

   Promoter

   CBh

   Available Since

   Sept. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET15b p97-Strep-His E314Amb

   Plasmid

   #169021

   Purpose

   Expresses p97-Strep-His E314Amb (incorporates noncanonical amino acid at position 314 via amber suppression) in bacteria

   Depositor

   Hemmo Meyer

   Article

   Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

   Insert

   p97
   

   Tags

   His and SBP

   Expression

   Bacterial

   Mutation

   E314TAG

   Promoter

   T7

   Available Since

   July 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-CMV-Fyn-cGASΔN-HA

   Plasmid

   #130922

   Purpose

   Expresses human cGASΔN (aa160-522)-HA with an N terminal fusion of the Fyn dual acylation motif (aa1-16); Puromycin selection marker

   Depositor

   Jonathan Kagan

   Article

   Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

   Insert

   Fyn-ΔNcGAS
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   cGAS N terminus (aa1-159) replaced with the dual …

   Promoter

   CMV

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFL His-I3 V41A / W43A

   Plasmid

   #113515

   Purpose

   Expression of His-I3 V41A / W43 A in insect cells (Baculovirus system)

   Depositor

   Hemmo Meyer

   Article

   Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

   Insert

   I3 (PPP1R11 Human)
   

   Tags

   6*His

   Expression

   Insect

   Mutation

   V41A /W43A

   Available Since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTE3314

   Plasmid

   #107530

   Purpose

   Expresses human codon optimized FnCpf1(RVR mutant) in mammalian cells.

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

   Insert

   hFnCpf1(RVR)
   

   Tags

   3xHA and NLS

   Expression

   Mammalian

   Mutation

   N607R, K613V, N617R

   Promoter

   CMV

   Available Since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTE3319

   Plasmid

   #107533

   Purpose

   Expresses human codon optimized MbCpf1(RR mutant) in mammalian cells.

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

   Insert

   hMbCpf1(RR)
   

   Tags

   3xHA and NLS

   Expression

   Mammalian

   Mutation

   N576R, K637R

   Promoter

   CMV

   Available Since

   April 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTE3316

   Plasmid

   #107532

   Purpose

   Expresses human codon optimized FnCpf1(RR mutant) in mammalian cells.

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

   Insert

   hFnCpf1(RR)
   

   Tags

   3xHA and NLS

   Expression

   Mammalian

   Mutation

   N607R, K671R

   Promoter

   CMV

   Available Since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTE3317

   Plasmid

   #107531

   Purpose

   Expresses human codon optimized MbCpf1(RVR mutant) in mammalian cells.

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

   Insert

   hMbCpf1(RVR)
   

   Tags

   3xHA and NLS

   Expression

   Mammalian

   Mutation

   N576R, K582V, N586R

   Promoter

   CMV

   Available Since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTE3179

    Plasmid

    #107528

    Purpose

    Expresses Mb crRNA and mCherry in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Inserts

    Mb crRNA
    mCherry
    

    Expression

    Mammalian

    Promoter

    CBh and human U6

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKK-TEV-mCardinal

    Plasmid

    #105798

    Purpose

    Expression of your protein of interest in fusion with red fluorescent protein at the C-terminus (cleavable by TEV), (PMID: 24633408). mCardinal is suitable for deep-tissue imaging.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    TEV-mCardinal

    Expression

    Mammalian

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKK-TEV-mCerulean

    Plasmid

    #105790

    Purpose

    Expression of your protein of interest in fusion with cyan fluorescent protein at the C-terminus (cleavable by TEV). Useful for FRET experiments (PMID: 14990965, 17040988, 22264545).

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    TEV-mCerulean

    Expression

    Mammalian

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKK-mCardinal-TEV

    Plasmid

    #105781

    Purpose

    Expression of your protein of interest in fusion with red fluorescent protein at the N-terminus (cleavable by TEV), (PMID: 24633408). mCardinal is suitable for deep-tissue imaging.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    mCardinal-TEV

    Expression

    Mammalian

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKK-mCerulean-TEV

    Plasmid

    #105773

    Purpose

    Expression of your protein of interest in fusion with cyan fluorescent protein at the N-terminus (cleavable by TEV). Useful for FRET experiments (PMID: 14990965, 17040988, 22264545).

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    mCerulean-TEV

    Expression

    Mammalian

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pQCXIX_sh_hMIB2_#9

    Plasmid

    #75255

    Purpose

    retroviral shRNA vector against human MIB2, expresses GFP for transduction control

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    shRNA against MIB2 (human) (MIB2 Human)
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Promoter

    mU6

    Available Since

    June 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-Hmga2 shMut m7

    Plasmid

    #52037

    Purpose

    encodes shMut HMGA2 m7

    Depositor

    Julian Downward

    Article

    Kumar et al Nature. 2014 Jan 9;505(7482):212-7. doi:

    Insert

    Hmga2 (Hmga2 Mouse)
    

    Expression

    Mammalian

    Mutation

    shRNA binding site mutated, all seven let-7 sites…

    Promoter

    CMV

    Available Since

    June 10, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA3.1-Hmga2 shMut ATG wt

    Plasmid

    #52038

    Purpose

    encodes shMut HMGA2 ATG wt

    Depositor

    Julian Downward

    Article

    Kumar et al Nature. 2014 Jan 9;505(7482):212-7. doi:

    Insert

    Hmga2 (Hmga2 Mouse)
    

    Expression

    Mammalian

    Mutation

    shRNA binding site mutated, first ATG mutated

    Promoter

    CMV

    Available Since

    June 10, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA3.1-Hmga2 shMut ATG m7

    Plasmid

    #52039

    Purpose

    encodes shMut HMGA2 ATG m7

    Depositor

    Julian Downward

    Article

    Kumar et al Nature. 2014 Jan 9;505(7482):212-7. doi:

    Insert

    Hmga2 (Hmga2 Mouse)
    

    Expression

    Mammalian

    Mutation

    shRNA binding site mutated, first ATG mutated, al…

    Promoter

    CMV

    Available Since

    June 10, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pPB-cT3G-cERP2-RUNX2

    Plasmid

    #192926

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of RUNX2

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    RUNX2 (RUNX2 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    TET3G-miniCMV

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-N-Flag-NLRP3

    Plasmid

    #75127

    Purpose

    Expresses N-terminal Flag-tagged NLRP3 in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

    Insert

    NLR family, pyrin domain containing 3 (Nlrp3 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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