We narrowed to 7,591 results for: Lif;

1. pCA528 IST1 (322-366)

   Plasmid

   #193038

   Purpose

   Bacterial expression plasmid for IST1 (322-366), the minimal construct with full binding to CAPN7 MIT domains.

   Depositor

   Wesley Sundquist

   Article

   Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

   Insert

   IST1 (IST1 Human)
   

   Use

   Tags

   6xHis-Sumo

   Expression

   Bacterial

   Mutation

   Residues 322-366

   Promoter

   T7

   Available since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-CAPN7-OSF

   Plasmid

   #193041

   Purpose

   Mammalian expression plasmid encoding full-length CAPN7 with C-terminal OSF tag (One-Strep-FLAG)

   Depositor

   Wesley Sundquist

   Article

   Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

   Insert

   CAPN7 (CAPN7 Human)
   

   Use

   Tags

   OSF (One-Strep-FLAG)

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken Beta-actin

   Available since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCA528 CAPN7 L61D (1-165)

   Plasmid

   #193033

   Purpose

   Bacterial expression plasmid for CAPN7 (1-165) tandem MIT domains with L61D mutation

   Depositor

   Wesley Sundquist

   Article

   Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

   Insert

   CAPN7 (CAPN7 Human)
   

   Use

   Tags

   6xHis-Sumo

   Expression

   Bacterial

   Mutation

   L61D, Residues 1-165

   Promoter

   T7

   Available since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCA528 CHMP6 145-201

   Plasmid

   #104597

   Purpose

   Expresses C-terminal 145-201 residues of CHMP6 with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-11

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   CHMP6 residues 145-201 (CHMP6 Human)
   

   Use

   Tags

   His-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCA528 CHMP7 366-453

   Plasmid

   #104598

   Purpose

   Expresses C-terminal 366-453 residues of CHMP7 with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-12

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   CHMP7 residues 366-453 (CHMP7 Human)
   

   Use

   Tags

   His-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCA528 CHMP4A 153-222

   Plasmid

   #108256

   Purpose

   Expresses C-terminal 153-222 residues of CHMP4A with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-6

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   CHMP4A residues 153-222 (CHMP4A Human)
   

   Use

   Tags

   His-SUMO

   Expression

   Bacterial

   Mutation

   Contains nonnative Gly-Cys for fluorescent labeli…

   Promoter

   T7

   Available since

   Nov. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMRXIP-GFP-TAPP1(PHD)

   Plasmid

   #221757

   Purpose

   Stable expression of GFP-TAPP1(PHD) in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

   Insert

   PLEKHA1 (PLEKHA1 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   tandem TAPP1 (PHD) (amino acids 184-304)

   Promoter

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMRXIP-Btk1(PHD)-GFP

   Plasmid

   #221756

   Purpose

   Stable expression of Btk1(PHD)-GFP in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

   Insert

   Btk (BTK Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   amino acids 1–177

   Promoter

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMRXIP-GFP-ING2(PHD)

   Plasmid

   #221751

   Purpose

   Stable expression of GFP-ING2(PHD) in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

   Insert

   ING2 (ING2 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   two ING2(PlantHD) domains (amino acids 190–280)

   Promoter

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-XPACK-DNAJC5 (L115R)- HaloTag

    Plasmid

    #205712

    Purpose

    Mammalian expression of XPACK-DNAJC5 (L115R)-HaloTag

    Depositor

    Randy Schekman

    Article

    Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

    Insert

    DNAJC5 (DNAJC5 Human)
    

    Use

    Tags

    HaloTag and XPACK (GCINSKRKD)

    Expression

    Mammalian

    Mutation

    L115R

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-DEAD XPACK-DNAJC5 (L115R)

    Plasmid

    #205718

    Purpose

    Mammalian expression of DEAD XPACK-DNAJC5 (L115R)

    Depositor

    Randy Schekman

    Article

    Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

    Insert

    DNAJC5 (DNAJC5 Human)
    

    Use

    Tags

    Dead XPACK (GCINIKRKD)

    Expression

    Mammalian

    Mutation

    L115R

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pQE-80L 2xHis-LRRK2 Armadillo-K17A

    Plasmid

    #186019

    Purpose

    2xHis-LRRK2 Armadillo-K17A

    Depositor

    Suzanne Pfeffer

    Article

    Vides et al Elife. 2022 Sep 23;11:e79771. doi: 10.75

    Insert

    2xHis-LRRK2 Armadillo-K17A (LRRK2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pQE-80L 2xHis-LRRK2 Armadillo- K18A

    Plasmid

    #186020

    Purpose

    2xHis-LRRK2 Armadillo- K18A

    Depositor

    Suzanne Pfeffer

    Article

    Vides et al Elife. 2022 Sep 23;11:e79771. doi: 10.75

    Insert

    2xHis-LRRK2 Armadillo- K18A (LRRK2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV Flag-LRRK2 K17A/K18A

    Plasmid

    #186012

    Purpose

    Flag-LRRK2 K17A/K18A

    Depositor

    Suzanne Pfeffer

    Article

    Vides et al Elife. 2022 Sep 23;11:e79771. doi: 10.75

    Insert

    Flag-LRRK2 K17A/K18A (LRRK2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV1-DIO-eGFP-pvRPL10a

    Plasmid

    #202542

    Purpose

    Cr-dependent (DIO) expression of eGFP-tagged ribosomal subunit that uses the prairie vole RPL10a gene sequence under the human synapsin promoter.

    Depositor

    Zoe Donaldson

    Article

    Sadino et al Elife. 2023 Feb 28;12:e80517. doi: 10.75

    Insert

    RPL10a subunit of the ribosome
    

    Use

    AAV and Cre/Lox

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    optimized for prairie vole DNA sequence and optim…

    Promoter

    human synapsin

    Available since

    Aug. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-CHMP2A-myc, L216D/L219D

    Plasmid

    #180645

    Purpose

    Mammalian expression of CHMP2A with L216D/L219D mutations. Has C-terminal Myc tag. Internal ID:WISP20-13.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    CHMP2A (CHMP2A Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    L216D, L219D

    Promoter

    pCAG

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-OSF-KATNA1 R14A

    Plasmid

    #160052

    Purpose

    Mammalian expression vector for KATNA1 (KATANIN-P60) R14A mutant with N-terminal One-Strep-Flag tag. Internal ID: WISP20-19.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    KATNA1 (KATNA1 Human)
    

    Use

    Tags

    One-Strep-Flag (OSF)

    Expression

    Mammalian

    Mutation

    R14A mutation

    Promoter

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-OSF-KATNA1 V55D

    Plasmid

    #160053

    Purpose

    Mammalian expression vector for KATNA1 (KATANIN-P60) V55D mutant with N-terminal One-Strep-Flag tag. Internal ID: WISP20-17.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    KATNA1 (KATNA1 Human)
    

    Use

    Tags

    One-Strep-Flag (OSF)

    Expression

    Mammalian

    Mutation

    V55D mutation

    Promoter

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCA528 KATNA1 MIT residues 1-79, V55D

    Plasmid

    #180615

    Purpose

    Bacterial expression for KATNA1 MIT domains, residues 1-79. Has V55D mutation. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-23.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    KATNA1 (KATNA1 Human)
    

    Use

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    Residues 1-79, V55D mutation

    Promoter

    T7

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUC19-π-EB1_IRES-mCherry

    Plasmid

    #197427

    Purpose

    Homology-directed repair template targeting IRES-based π-element to MAPRE1 exon 5

    Depositor

    Torsten Wittmann

    Article

    Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

    Insert

    IRES-based π-element with HDR templates flanking MAPRE1 exon 5 insertion site (MAPRE1 Human)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    GCN4 leucine zipper, LOV2, Zdk1, and mCherry

    Expression

    Mutation

    Promoter

    Available since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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