Addgene

We narrowed to 3,106 results for: actin

Showing: 2901 - 2920 of 3106 results

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pMRX-IP-hWIPI2B-3×FLAG

Plasmid

#215510

Purpose

Expresses FLAG tagged human WIPI2B.

Depositor

Noboru Mizushima

Article

Shimizu et al Hum Mol Genet. 2023 Aug 7;32(16):2623-26

Insert

WD-repeat protein interacting with phosphoinositides 2B (WIPI2 Human)

Use

Retroviral

Tags

3×FLAG

Expression

Mammalian

Mutation

Promoter

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RIPK3[S199D]-FLAG

Plasmid

#199315

Purpose

mutated phosphorylation site (kinase acitivity permanent active)

Depositor

Adrian Press

Article

Hoff et al Cell Death Dis. 2023 Apr 18;14(4):275. d

Insert

Receptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

changed Serine 199 to Aspartic Acid

Promoter

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RIPK3[S199A]-FLAG

Plasmid

#199313

Purpose

mutated phosphorylation site (kinase acitivity inhibited)

Depositor

Adrian Press

Article

Hoff et al Cell Death Dis. 2023 Apr 18;14(4):275. d

Insert

Receptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

changed Serine 199 to Alanine

Promoter

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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Venus-Bik-del72-136-pEGFP-C3

Plasmid

#191411

Purpose

Transfection of this plasmid will express "VBik-d72-136" (Venus fused to the N-terminus of the BH3-only protein, Bik that has amino acids 72-136 deleted).

Depositor

David Andrews

Insert

Bcl-2-interacting killer (BIK Human)

Use

Tags

Venus

Expression

Mammalian

Mutation

with deletion of amino acids 72-136 (del72-136) i…

Promoter

CMV

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuet1-His-TEV-OTC_d1_Ub

Plasmid

#170729

Purpose

Scaﬀold design for studying ubiquitin interacting proteins by cryo-EM.

Depositor

Shang-Te Danny Hsu

Article

Chiu et al Biochemistry. 2021 Apr 13;60(14):1075-10

Insert

E. coli ornithine transcarbamylase (OTC), fused to human ubiquitin

Use

Tags

6xHis-TEV

Expression

Bacterial

Mutation

The C-terminus of OTC was truncated by removing o…

Promoter

T7

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pFASTbac-HisGST-PTPIP51(36-470)

Plasmid

#170531

Purpose

Expresses Cleavable HisGST-tagged PTPIP51 (36-470) in Sf9

Depositor

Byung Il Lee

Article

Yeo et al EMBO Rep. 2021 Jun 4;22(6):e51323. doi:

Insert

Protein tyrosine phosphatase interacting protein 51 (RMDN3 )

Use

Tags

GST tag and His tag

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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D52_Ld_322130

Plasmid

#135865

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

TLR4 regulator and MIR-interacting MSAP, putative

Use

Tags

biotinylation sequence and His tag

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

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pPKm-113

Plasmid

#90495

Purpose

pcDNA3 -pCMV - MTAD - PIF6, expresses the Phytochrome interacting factor 6 (PIF6) and MTAD, under CMV promoter

Depositor

Todd Coleman

Article

Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

Insert

MTAD-PIF6

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pcdna3 T7 Jip1b(C631S, C643S and C699T)

Plasmid

#52128

Purpose

a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.

Depositor

Roger Davis

Article

Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.

Insert

JNK interacting protein-1b (JIP-1b)(M1-5) (Mapk8ip1 Mouse)

Use

Tags

T7

Expression

Mammalian

Mutation

C631S, C643S and C699T

Promoter

Available since

June 11, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3 T7 Jip1b(471-707)

Plasmid

#52428

Purpose

a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.

Depositor

Roger Davis

Article

Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.

Insert

JNK interacting protein-1b (JIP-1b)(471-707) ) (Mapk8ip1 Mouse)

Use

Tags

T7

Expression

Mammalian

Mutation

deleted amino acid 1-470

Promoter

Available since

April 29, 2014

Availability

Academic Institutions and Nonprofits only
pcdna3 T7 Jip1b(282-707)

Plasmid

#52126

Purpose

a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.

Depositor

Roger Davis

Article

Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.

Insert

JNK interacting protein-1b (JIP-1b)(282-707)) (Mapk8ip1 Mouse)

Use

Tags

T7

Expression

Mammalian

Mutation

deleted amino acid 1-281

Promoter

Available since

April 18, 2014

Availability

Academic Institutions and Nonprofits only
pCDNA3 T7 Jip1(1-470)

Plasmid

#51724

Purpose

" JIP-1 may suppress signal transduction by the JNK pathway"

Depositor

Roger Davis

Article

Dickens et al Science. 1997 Aug 1. 277(5326):693-6.

Insert

Jnk interacting protein 1 (Mapk8ip1 Mouse)

Use

Tags

T7

Expression

Mammalian

Mutation

deleted 471-660

Promoter

Available since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

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pCDNA3 T7 Jip1(471-660)

Plasmid

#51766

Purpose

" JIP-1 may suppress signal transduction by the JNK pathway"

Depositor

Roger Davis

Article

Dickens et al Science. 1997 Aug 1. 277(5326):693-6.

Insert

Jnk interacting protein 1 (Mapk8ip1 Mouse)

Use

Tags

T7

Expression

Mammalian

Mutation

deleted 1-470

Promoter

Available since

March 14, 2014

Availability

Academic Institutions and Nonprofits only
pcdna3 T7Jip1(1-126)

Plasmid

#51702

Purpose

" JIP-1 may suppress signal transduction by the JNK pathway"

Depositor

Roger Davis

Article

Dickens et al Science. 1997 Aug 1. 277(5326):693-6.

Insert

Jnk interacting protein 1 (Mapk8ip1 Mouse)

Use

Tags

T7

Expression

Mammalian

Mutation

deleted 127-660

Promoter

Available since

March 14, 2014

Availability

Academic Institutions and Nonprofits only
pCDNA3 T7 Jip1(1-281)

Plasmid

#51700

Purpose

" JIP-1 may suppress signal transduction by the JNK pathway"

Depositor

Roger Davis

Article

Dickens et al Science. 1997 Aug 1. 277(5326):693-6.

Insert

Jnk interacting protein 1 (Mapk8ip1 Mouse)

Use

Tags

T7

Expression

Mammalian

Mutation

deleted 282-660

Promoter

Available since

March 14, 2014

Availability

Academic Institutions and Nonprofits only
KHBD00596

Plasmid

#39677

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG6061 (mip120 Fly)

Use

Gateway donr vector

Tags

Expression

Mutation

Promoter

none

Available since

Aug. 23, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00080

Plasmid

#34237

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG12767 (Dip3 Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
CIBN-CAAX

Plasmid

#79574

Purpose

expression of N-terminal portion of CIB1 with a C-terminal CAAX box from KRras for plasma-membrane targeting

Depositor

Pietro De Camilli, Olof Idevall-Hagren

Article

Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

Insert

CIBN (CIB1 Mustard Weed)

Use

Tags

C-terminal CAAX-box

Expression

Mammalian

Mutation

K93A, R94A, K106A, K107A (please see depositor co…

Promoter

CMV

Available since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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2xmyc-JIP4

Plasmid

#164633

Purpose

Expresses JIP4 in mammalian cells with a 2xmyc tag

Depositor

Mark Cookson

Article

Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

Insert

JIP4 (SPAG9 Human)

Use

Tags

2xmyc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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SID4x-dCas9-KRAB

Plasmid

#106399

Purpose

Expresses catalytically inactive dCas9 with N-terminal fusion of Sin3a Interacting Domain (SID) and C-terminal fusion of repressive KRAB domain

Depositor

Jason Gertz

Article

Carleton et al Cell Syst. 2017 Oct 25;5(4):333-344.e5.

Inserts

SID4x (MXD1 Human)
KRAB (ZNF10 Human)

Use

Tags

FLAG, HA, and NLS

Expression

Mammalian

Mutation

Promoter

Available since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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