We narrowed to 8,756 results for: ef1a

1. MCP-MSN

   Plasmid

   #210704

   Purpose

   This plasmid express EF1a promoter driven MCP-MSN transactivation module

   Depositor

   Isaac Hilton

   Article

   Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

   Insert

   MCP fused to transactivation domain from Human transcription factor MRTF-A, STAT-1 and NRF2
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   N55K in MCP

   Promoter

   EF1a

   Available since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. gRNA-10Xcapture-PuroR

   Plasmid

   #211496

   Purpose

   Plasmid for the expression of SAM-compatible gRNA for CRISPRa with capturer sequence for 10X Genomics sequencing

   Depositor

   Antonella Fidanza, Lesley Forrester

   Article

   Petazzi et al Elife. 2024 Dec 23;13:RP94884. doi: 10.7

   Inserts

   U6-gRNA
   Ef1a-PuroR
   

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a and U6

   Available since

   Dec. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMK1254

   Plasmid

   #133057

   Purpose

   Lentiviral vector with EF1A promoter constitutively expressing a single insert containing the Tau.K18(P301L/V337M)-mRuby2 fusion protein.

   Depositor

   Martin Kampmann

   Article

   Chen et al J Biol Chem. 2019 Oct 2. pii: RA119.0094

   Insert

   Tau.K18(LM)
   

   Use

   Lentiviral

   Tags

   mRuby2

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   Jan. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. MCP-eN3x9

   Plasmid

   #210706

   Purpose

   This plasmid express EF1a promoter driven MCP-eN3x9 transactivation module

   Depositor

   Isaac Hilton

   Article

   Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

   Insert

   MCP fused to transactivation domain comprising eNRF2 and 3x 9aa Transactivation domain
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   N55K in MCP

   Promoter

   EF1a

   Available since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMK1253

   Plasmid

   #133058

   Purpose

   Lentiviral vector with EF1A promoter constitutively expressing a single insert containing the Tau.K18(P301L/V337M)-Clover2 fusion protein.

   Depositor

   Martin Kampmann

   Article

   Chen et al J Biol Chem. 2019 Oct 2. pii: RA119.0094

   Insert

   Tau.K18(LM)
   

   Use

   Lentiviral

   Tags

   mClover2

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   Jan. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA5FRT-EF-Pdgfrbeta-EGFPN

   Plasmid

   #66790

   Purpose

   Expression of murine PDGFRbeta tagged with GFP under the control of EF1a promoter

   Depositor

   Lotte Pedersen

   Article

   Nielsen et al J Cell Sci. 2015 Oct 1;128(19):3543-9. d

   Insert

   PDGFRbeta (Pdgfrb Mouse)
   

   Use

   Tags

   C-term EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. MCP-NMS

   Plasmid

   #210705

   Purpose

   This plasmid express EF1a promoter driven MCP-NMS transactivation module

   Depositor

   Isaac Hilton

   Article

   Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

   Insert

   MCP fused to transactivation domain from Human transcription factor NRF2, MRTF-A and STAT1
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   N55K in MCP

   Promoter

   EF1a

   Available since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pmCTIP2-N174

   Plasmid

   #60858

   Purpose

   Expresses CTIP2 under the EF1a promoter

   Depositor

   Andrew Yoo

   Article

   Victor et al Neuron. 2014 Oct 22;84(2):311-23. doi: 1

   Insert

   CTIP2 isoform b (Bcl11b Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Dec. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLV-mCherry-hCdt1(1-100)ΔCy

   Plasmid

   #193759

   Purpose

   Expresses N-CRL4Cdt2 reporter(mCherry-hCdt1(1-100)ΔCy)

   Depositor

   Tobias Meyer

   Article

   Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

   Insert

   mCherry-hCdt1(1-100)ΔCy (CDT1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   human Cdt1 amino acids 1-100 w/ ΔCy mutation (aa6…

   Promoter

   EF1a

   Available since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLL8 lenti-D10Acas9-3xflag-blast

    Plasmid

    #112134

    Purpose

    lenti vector encoding D10Acas9-3xflag with T2A Blastcidin resistance marker (EF1a-NLS-D10ACas9-3Xflag-T2A-Blast-WPRE)

    Depositor

    Fei-Long Meng

    Article

    Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

    Insert

    D10Acas9-3xflag-blast
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A mutant in Cas9

    Promoter

    EF1a

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLV-mTurquoise-MLC-IRES-neo

    Plasmid

    #85145

    Purpose

    lentiviral expression of myosin light chain (MLC, MYL9), N-terminally tagged with mTurquoise

    Depositor

    Tobias Meyer

    Article

    Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

    Insert

    mTurquoise-MLC (myosin regulatory light chain, N-terminally tagged with mTurquoise (MYL9 Human)
    

    Use

    Lentiviral

    Tags

    mTurquoise

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV-IV-GFPL10

    Plasmid

    #98704

    Purpose

    Cre-dependently express GFPL10 fusion using Introvert Switch

    Depositor

    Alexander Nectow, Jeffrey Friedman

    Article

    Nectow et al Cell. 2017 Jul 27;170(3):429-442.e11. do

    Insert

    GFPL10
    

    Use

    AAV and Cre/Lox

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    L10: 2609 C->T to remove EcoRI site

    Promoter

    EF1a

    Available since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. crTet in pSLQ8453

    Plasmid

    #183965

    Purpose

    U6-crTet-EF1a-Puro-T2A-BFP-WPRE

    Depositor

    Stanley Qi

    Article

    Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

    Insert

    crTet
    

    Use

    Lentiviral

    Tags

    EF1a-Puro-T2A-BFP

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. MCP-3x9aaTAD

    Plasmid

    #210707

    Purpose

    This Plasmid express EF1a promoter driven MCP-3x 9aa transactivation module

    Depositor

    Isaac Hilton

    Article

    Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

    Insert

    MCP fused to 3x 9aa transactivation domain derived from MRTF-B and MYOCD1
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    N55K in MCP

    Promoter

    EF1a

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLV-puro-N-Flag-Snrnp40

    Plasmid

    #134253

    Purpose

    Lentivector encoding Flag-tagged Snrnp40

    Depositor

    Bruce Beutler

    Article

    Zhang et al Nat Immunol. 2019 Oct;20(10):1322-1334.

    Insert

    Snrp40 (Snrnp40 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    May 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PB-iRFP-STOP-ReNL

    Plasmid

    #113965

    Purpose

    Piggybac transposon plasmid CRISPR gene deletion activatable fluorescence. Constitutive iRFP670 under EF1A promoter, CMV promoter with two SV40 polyA followed by red-enhanced nanolantern (ReNL)

    Depositor

    Jordan Green

    Article

    Rui et al Sci Adv. 2019 Dec 6;5(12):eaay3255. doi:

    Inserts

    iRFP670
    ReNL
    

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV - SV40-PolyA - SV40-PolyA and EF1A

    Available since

    Dec. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. lentiSAMv2

    Plasmid

    #75112

    Purpose

    lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2, dCas9-VP64, and blast resistance marker. Contains BsmBI sites for insertion of spacer sequences.

    Depositor

    Feng Zhang

    Article

    Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and EF1A

    Available since

    May 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHAGE-IRES-puro-NLS-dPguCas13b-EGFP-NLS-3xFlag

    Plasmid

    #132400

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

    Insert

    dPguCas13b
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    H151A; H1121A

    Promoter

    Available since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHAGE-IRES-puro-NLS-dRanCas13b-EGFP-NLS-3xFlag

    Plasmid

    #132409

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

    Insert

    dRanCas13b
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    H147A; H1044A

    Promoter

    Available since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pXPR_120

    Plasmid

    #96917

    Purpose

    for CRISPRa, lentiviral expression of dCas9-VPR 2A BlastR

    Depositor

    David Root, John Doench

    Article

    Najm et al Nat Biotechnol. 2018 Feb;36(2):179-189.

    Insert

    dCas9
    

    Use

    Lentiviral

    Tags

    VPR

    Expression

    Mammalian

    Mutation

    D10A, N863A

    Promoter

    EF1a

    Available since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge