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AAV-FLEX-EGFP-mir30(Scn9a-scrambled)

Plasmid

#79671

Purpose

Scrambled control for Cre-dependent knockdown of Scn9a (Nav1.7)

Depositor

Scott Sternson

Publication

Branco et al Cell. 2016 Jun 16;165(7):1749-61. doi: 1

Insert

EGFP

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

CAG

Available since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pKO (Hyg)

Plasmid

#110087

Purpose

Suicide vector for generation of gene deletions in mycobacteria. Contains the two genes sacB and galK for negative selection.

Depositor

Markus Seeger

Publication

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Use

Bacterial knockout generation

Available since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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pKO (Apr)

Plasmid

#110088

Purpose

Suicide vector for generation of gene deletions in mycobacteria. Contains the two genes sacB and galK for negative selection.

Depositor

Markus Seeger

Publication

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Use

Bacterial knockout generation

Available since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAP24

Plasmid

#70190

Purpose

Modular vector for secreted luciferase reporter expression

Depositor

Wiep Klaas Smits

Publication

Oliveira Paiva et al ACS Synth Biol. 2016 Jun 23.

Insert

sLucopt

Use

Synthetic Biology

Expression

Bacterial

Promoter

Ptet

Available since

June 27, 2016

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1(+)-myc-SNAP-GP135

Plasmid

#50376

Purpose

Express myc and SNAP-tagged GP135 in mammmalian cells

Depositor

Reika Watanabe

Publication

Castillon et al Mol Biol Cell. 2013 Jun;24(12):2021-33.

Insert

SNAP

Expression

Mammalian

Available since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pCDNA_B2AR-i-pep

Plasmid

#47436

Purpose

FRET sensor for beta2-adrenergic receptor

Depositor

Sivaraj Sivaramakrishnan

Publication

Malik et al J Biol Chem. 2013 Jun 14;288(24):17167-7

Insert

beta2-adrenergic receptor (ADRB2 Human)

Tags

10 nm ER/K helix, HA, i-pep, mCerulean, and mCitr…

Expression

Mammalian

Promoter

pCMV

Available since

Sept. 11, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pFRT/TO/HIS/FLAG/HA-SART1

Plasmid

#38087

Depositor

Markus Landthaler

Publication

Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

Insert

SART1 squamous cell carcinoma antigen recognized by T cells (SART1 Human)

Tags

HIS/FLAG/HA

Expression

Mammalian

Available since

Sept. 17, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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200 bp Axin2 basic

Plasmid

#25703

Depositor

Eric Fearon

Publication

Leung et al J Biol Chem. 2002 Jun 14. 277(24):21657-

Insert

Axin 2 promoter (AXIN2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available since

June 24, 2010

Availability

Academic Institutions and Nonprofits only
SQ171/pCSacB/ptRNA100

Bacterial strain

#155206

Purpose

SQ171 strain supported by pCSacB and ptRNA100 plasmids

Depositor

Alexander Mankin

Publication

Huang et al Nat Commun. 2020 Jun 9;11(1):2900. doi:

Bacterial resistance

Kanamycin

Available since

April 27, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pCMV-Nopt

Plasmid

#231704

Purpose

Mammalian expression vector encoding a codon-optimized N gene from vesicular stomatitis virus, strain Indiana.

Depositor

Ron Geller

Publication

Marques et al Sci Rep. 2024 Jun 25;14(1):14644. doi: 1

Insert

VSV N gene (codon-optimized ) (VSIVgp1 vesicular stomatitis virus)

Use

Vsv expression

Expression

Mammalian

Mutation

human codon-optimized

Promoter

CMV

Available since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-Popt

Plasmid

#231705

Purpose

Mammalian expression vector encoding a codon-optimized P gene from vesicular stomatitis virus, strain Indiana.

Depositor

Ron Geller

Publication

Marques et al Sci Rep. 2024 Jun 25;14(1):14644. doi: 1

Insert

VSV P gene (codon-optimized )

Use

Vsv expression

Expression

Mammalian

Mutation

human codon-optimized

Promoter

CMV

Available since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-Lopt

Plasmid

#231706

Purpose

Mammalian expression vector encoding a codon-optimized L gene from vesicular stomatitis virus, strain Indiana.

Depositor

Ron Geller

Publication

Marques et al Sci Rep. 2024 Jun 25;14(1):14644. doi: 1

Insert

VSV L gene (codon-optimized )

Use

Vsv expression

Expression

Mammalian

Mutation

human codon-optimized

Promoter

CMV

Available since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV gfaABC1D NES-jRCaMP1a

Plasmid

#171120

Purpose

Astrocyte AAV-mediated expression of jRCaMP1a, a red fluorescent calcium sensor protein.

Depositor

Thomas Oertner

Publication

Lohr et al Front Cell Neurosci. 2021 Jun 11;15:6901

Insert

jRCaMP1a

Use

AAV

Tags

His Tag, T7 Tag, XpressTag

Expression

Mammalian

Promoter

gfaABC1D

Available since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-mCherry-NLS

Plasmid

#165442

Purpose

Expresses nuclear mCherry from CMV promoter

Depositor

Esteban Engel

Publication

Maturana et al Gene Ther. 2021 Jun 28. pii: 10.1038/s41

Insert

mCherry-NLS

Use

AAV

Tags

NLS

Expression

Mammalian

Promoter

CMV

Available since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pEE391

Plasmid

#149281

Purpose

T-DNA encoding TRV2 with FT augmented gRNA targeting NbPDS3

Depositor

Daniel Voytas

Publication

Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

Insert

p35S:TRV2_NbPDS3sgRNA_FT:tNos

Expression

Plant

Promoter

35S

Available since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

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pEN_TT 3XFLAG-wtGATA6-3XAU1

Plasmid

#72612

Purpose

Gateway entry vector for an inducible N-terminally 3XFLAG-tagged and C-terminally AU1-tagged human wtGATA6

Depositor

Kevin Janes

Publication

Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

Insert

GATA binding protein 6 (GATA6 Human)

Use

Gateway Cloning

Tags

3XAU1 and 3XFLAG

Expression

Bacterial

Promoter

TRE tight promoter

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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pMRXIP-GFP-PI4K2A

Plasmid

#221760

Purpose

Stable expression of GFP-PI4K2A in mammalian cells

Depositor

Noboru Mizushima

Publication

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

PI4K2A (PI4K2A Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO.1C

Plasmid

#139470

Purpose

Replacing the RSV promoter of pLKO.1 with the CMV promoter improves lentiviral production with third-generation viral packaging system.

Depositor

David Cobrinik

Publication

Lee et al Biotechniques. 2020 Jun;68(6):349-352. d

Insert

stuffer

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

CMV

Available since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pAG504-frFAST

Plasmid

#160055

Purpose

Expresses frFAST in mammalian cells

Depositor

Arnaud Gautier

Publication

Li et al Angew Chem Int Ed Engl. 2020 Jun 22. doi

Insert

frFAST

Expression

Mammalian

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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Fgfr2-T2A-H2B-Halo donor

Plasmid

#113117

Purpose

Donor plasmid for knock-in T2A-H2B-Halo to the c-terminal of mouse Fgfr2 coding sequence

Depositor

Janet Rossant

Publication

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

Fgfr2 donor region including homology arms and T2A-H2B-Halo

Use

Synthetic Biology

Available since

Aug. 7, 2018

Availability

Academic Institutions and Nonprofits only

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