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Showing: 2901 - 2920 of 7226 results

  2. pTOPO-col10a1-KI-donor

    Plasmid
    #184874
    Purpose
    Donor template for knock-in of p2a-CreERT2 into the medaka col10a1 locus via CRISPR/Cas9-mediated homology directed repair
    Depositor
    Christoph Winkler
    Article
    Hui et al Development. 2022 May 20. pii: 275483. d
    Inserts
    Collagen10a1 5' homology arm
    Collagen10a1 3' homology arm
    p2a-CreERT2
    Myosin light chain 2 promoter
    eGFP
    Use
    CRISPR and Cre/Lox
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    184874-plasmid-map-sequence-id-363155

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  6. Myc-gamma3/gamma2SIL

    Plasmid
    #121433
    Purpose
    GABAA receptor expression (chimeric rat gamma 3 subunit whose large intracellular loop has been replaced with the large intracellular loop of the rat gamma 2 short subunit) Myc-tag near N-terminus
    Depositor
    Angel de Blas
    Insert
    GABRG3/GABRG2 SIL (Gabrg2, Gabrg3 Rat)
    Use
    Tags
    cMyc epitope EQKLISEEDL inserted between fourth a…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    121433-plasmid-map-sequence-id-237864

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  7. Myc-gamma1/gamma2SIL

    Plasmid
    #121432
    Purpose
    GABAA receptor expression (chimeric rat gamma 1 subunit whose large intracellular loop has been replaced with the large intracellular loop of the rat gamma 2 short subunit) Myc-tag near N-terminus
    Depositor
    Angel de Blas
    Insert
    GABRG1/GABRG2 SIL (Gabrg1, Gabrg2 Rat)
    Use
    Tags
    cMyc epitope EQKLISEEDL inserted between fourth a…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    121432-plasmid-map-sequence-id-237859

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  8. Sema3f(L,-)-Fc-His

    Plasmid
    #72148
    Purpose
    Expresses the Sema3F protein (truncated at cleavage site P3; ie, long and contains a 93 nt deletion in exon 3), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.
    Depositor
    Woj Wojtowicz
    Article
    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1
    Insert
    Sema3f (Sema3f Mouse)
    Use
    Tags
    Fc-His
    Expression
    Mammalian
    Mutation
    V319I
    Promoter
    CMV
    Available since
    Feb. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72148-plasmid-map-sequence-id-139007

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  19. pAV10.F3.loxP

    Plasmid
    #63200
    Purpose
    pCACS backbone (Amp), SpHIS5, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into chrVI: 97873-98803 (NotI or BciVI).
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Availability
    Academic Institutions and Nonprofits only
    63200-plasmid-map-sequence-id-104877

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  20. pAV10.HO5.loxP

    Plasmid
    #63206
    Purpose
    pCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into HO locus (NotI or BciVI).
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Availability
    Academic Institutions and Nonprofits only
    63206-plasmid-map-sequence-id-104906

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Showing: 2901 - 2920 of 7226 results