207,468 results

1. pCMV-EGFP-eDHFR(69K6)-MCS

   Plasmid

   #172100

   Purpose

   Mammalian expression of a protein of interest fused to the C-terminus of EGFP-eDHFR(69K6)

   Depositor

   Shinya Tsukiji

   Article

   Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

   Insert

   EGFP-eDHFR(69K6)-20aa
   

   Expression

   Mammalian

   Mutation

   eDHFR(69K6): Hexalysine (K6) sequence is inserted…

   Promoter

   CMV

   Available Since

   July 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pZR112_Lenti-SFFV-mCherry-2A-dCas9-VP64

   Plasmid

   #180263

   Purpose

   Lentiviral expression vector for dCas9-VP64 in mammalian cells

   Depositor

   Alexander Marson

   Article

   Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   Nuclease dead Cas9

   Promoter

   SFFV

   Available Since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCMMP-MCS-IRES-eGFP

   Plasmid

   #36953

   Depositor

   Bill Sugden

   Article

   Kennedy et al Mol Cell Biol. 2003 Oct;23(19):6901-8.

   Type

   Empty backbone

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 16, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Myc-E-Syt2

   Plasmid

   #66834

   Purpose

   Mammalian Expression, Bacterial Expression

   Depositor

   Pietro De Camilli

   Article

   Giordano et al Cell. 2013 Jun 20;153(7):1494-509. doi:

   Insert

   E-Syt2 (ESYT2 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Available Since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pZac2.1 hSynapsin1 NAPA-N SV40

   Plasmid

   #92282

   Purpose

   FRET probe; extracellular synapse targeted mCherry

   Depositor

   Baljit Khakh

   Article

   Octeau et al Neuron. 2018 Apr 4;98(1):49-66.e9. doi:

   Insert

   Neuron-astrocyte proximity assay - Neuronal
   

   Use

   AAV

   Promoter

   hSynapsin1

   Available Since

   April 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV-hSyn1-SIO-eOPN3-mScarlet-WPRE (AAV5)

   Viral Prep

   #125713-AAV5

   Purpose

   Ready-to-use AAV5 particles produced from pAAV-hSyn1-SIO-eOPN3-mScarlet-WPRE (#125713). In addition to the viral particles, you will also receive purified pAAV-hSyn1-SIO-eOPN3-mScarlet-WPRE plasmid DNA. Synapsin-driven, Cre-dependent expression of mosquito OPN3 rhodopsin in-frame with mScarlet. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Ofer Yizhar

   Article

   Mahn et al Neuron. 2021 May 10. pii: S0896-6273(21)

   Promoter

   Syn

   Tags

   mScarlet (Cre-dependent)

   Available Since

   Sept. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

7. pLminP_Luc2P_RE6

   Plasmid

   #90340

   Purpose

   STAT5 - Stat5 gene reporter

   Depositor

   Ramnik Xavier

   Article

   O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

   Insert

   Firefly luciferase
   

   Use

   Lentiviral

   Promoter

   STAT5

   Available Since

   May 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3-FLAG-MTOR-V2006I

   Plasmid

   #69012

   Purpose

   activating MTOR mutation

   Depositor

   David Sabatini

   Article

   Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

   Insert

   MTOR (MTOR Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   V2006I

   Promoter

   CMV

   Available Since

   Jan. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJ609-dCas9-KR-puro

   Plasmid

   #158478

   Purpose

   Express nuclease dead Cas9 fused to KillerRed chromophore in mammalian cells

   Depositor

   Brendan Price

   Article

   House et al PLoS One. 2020 Dec 17;15(12):e0237759. d

   Insert

   dCas9-KR
   

   Use

   CRISPR

   Tags

   KillerRed

   Expression

   Mammalian

   Mutation

   Cas9m4 (D10A+D839A+H840A+N863A)

   Promoter

   CMV

   Available Since

   Nov. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pet-15b-microID

    Plasmid

    #172881

    Purpose

    expresses microID in E. coli

    Depositor

    Julien Béthune

    Article

    Kubitz et al Commun Biol. 2022 Jul 4;5(1):657. doi: 1

    Insert

    microID
    

    Tags

    myc

    Expression

    Bacterial

    Mutation

    fragment ([aa2-171]) of aquifex aeolicus BirA wit…

    Promoter

    T7

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. myc EDC4 pCMVSport6

    Plasmid

    #86855

    Purpose

    mammalian expression of EDC4

    Depositor

    Sarah Slavoff

    Article

    D'Lima et al Nat Chem Biol. 2016 Dec 5. doi: 10.1038/

    Insert

    EDC4 (EDC4 Human)
    

    Tags

    myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSFFV_sfCherry2(11)_TagBFP

    Plasmid

    #82606

    Purpose

    Expresses sfCherry2(11) and TagBFP fusion in mammalian cells

    Depositor

    Bo Huang

    Article

    Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

    Insert

    sfCherry2(11)_TagBFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    changed Gly12 to Ala in sfCherry11+

    Promoter

    SFFV

    Available Since

    June 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLenti-SFNp-mRFP1

    Plasmid

    #238473

    Purpose

    Expresses higher mRFP1 fluorescence when NFkB is more active. Cloned into pLenti backbone

    Depositor

    Christopher Nelson

    Insert

    SFNp-mRFP1
    

    Promoter

    SFNp

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRK7-HA-S6K1-F5A

    Plasmid

    #8986

    Depositor

    John Blenis

    Article

    Schalm et al Curr Biol. 2002 Apr 16. 12(8):632-9.

    Insert

    S6K1 (Rps6kb1 Rat)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    F5A

    Available Since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

15. mEmerald-ZO1-C-14

    Plasmid

    #54316

    Purpose

    Localization: Tight Junctions, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Insert

    ZO1 (TJP1 Human)
    

    Tags

    mEmerald

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 16, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. mH2A1.1-CT-MYC

    Plasmid

    #45166

    Depositor

    Hunt Willard, Brian Chadwick

    Article

    Chadwick et al Hum Mol Genet. 2001 May 1;10(10):1101-13

    Insert

    H2Afy (MACROH2A1 Human)
    

    Tags

    HIS and MYC

    Expression

    Mammalian

    Available Since

    July 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. plentiCRISPR-v2 Puro EIF2AK1/HRI_sgRNA

    Plasmid

    #218529

    Purpose

    sgRNA targeting human EIF2AK1/HRI

    Depositor

    Kivanc Birsoy

    Article

    Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

    Insert

    EIF2AK1 (EIF2AK1 Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTwist-SFFV-NFE2L18ND-Puro

    Plasmid

    #181918

    Purpose

    Lentiviral plasmid that directs the expression of mutant NFE2L1/Nrf1 where 8 putative N-glycosites are mutated from asparagine to aspartic acid in mammalian cells.

    Depositor

    Scott Dixon

    Article

    Forcina et al Proc Natl Acad Sci U S A. 2022 Mar 15;11

    Insert

    NFE2L1 (NFE2L1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    8 putative N-glycoyslation sites converted from a…

    Promoter

    SFFV

    Available Since

    April 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3-TORCAR-NLS

    Plasmid

    #64931

    Purpose

    Nuclear-targeted FRET biosensor for monitoring mTORC1 kinase activity.

    Depositor

    Jin Zhang

    Article

    Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

    Insert

    TORCAR-NLS (EIF4EBP1 Synthetic, Human)
    

    Tags

    Cerulean, Nuclear localization signal (NLS), and …

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TFORF0121

    Plasmid

    #141526

    Purpose

    Lentiviral vector for overexpressing the BARX2 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    BARX2 (BARX2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. S2060

    Bacterial Strain

    #105064

    Purpose

    host strain for phage assisted continuous evolution

    Depositor

    David Liu

    Article

    Hubbard et al Nat Methods. 2015 Oct;12(10):939-42. doi

    Bacterial Resistance

    Streptomycin and tetracycline

    Available Since

    March 23, 2018

    Availability

    Academic Institutions and Nonprofits only

22. A*24:02:01

    Plasmid

    #203295

    Purpose

    Overexpress HLA allele

    Depositor

    Derin Keskin, Catherine Wu

    Article

    Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

    Insert

    A*24:02:01 (HLA-A Human)
    

    Expression

    Mammalian

    Available Since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. JAK2 (V617F)-pcw107-V5

    Plasmid

    #64610

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    David Sabatini, Kris Wood

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    JAK2 (JAK2 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Mutation

    V617F

    Promoter

    PGK

    Available Since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAAV-CaMKIIa-eArchT3.0-P2A-EGFP-WPRE-hGHpA

    Plasmid

    #51110

    Purpose

    Forebrain principal neuron expression of eArchT3,0 with physically uncoupled EGFP fluorophore

    Depositor

    Jonathan Ting

    Insert

    eArchT3.0
    

    Use

    AAV

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Promoter

    CaMKIIa

    Available Since

    April 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3-FLAG-MTOR-E1799K

    Plasmid

    #69009

    Purpose

    activating MTOR mutation

    Depositor

    David Sabatini

    Article

    Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

    Insert

    MTOR-E1799K (MTOR Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    change Glutamate 1799 to Lysine

    Available Since

    Dec. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pENTR223-BORF2

    Plasmid

    #195834

    Purpose

    Gateway Entry Clone of Epstein-Barr virus BORF2

    Depositor

    Eric Johannsen

    Article

    Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    BORF2
    

    Use

    Gateway entry vector

    Available Since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pC1-HyPer-Red

    Plasmid

    #48249

    Purpose

    Genetically encoded hydrogen peroxide indicator with red fluorescence.

    Depositor

    Vsevolod Belousov

    Article

    Ermakova et al Nat Commun. 2014 Oct 21;5:5222. doi: 10.

    Insert

    HYPER-RED
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Lp-BFPnls-LP-Cherry-T2A-NTR2.0

    Plasmid

    #219785

    Purpose

    Used for generating a transgenic line for inducing NTR2.0 expression in Cre positive cells after conversion

    Depositor

    Prayag Murawala

    Article

    García-García et al bioRxiv 2025.03.30.645595; doi: 10.1101/

    Insert

    mTagBFP2
    

    Tags

    3x SV40 NLS

    Available Since

    April 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCMV-PTEN-C124S

    Plasmid

    #28300

    Depositor

    Maria-Magdalena Georgescu

    Article

    Takahashi et al EMBO J. 2006 Feb 22. 25(4):910-20.

    Insert

    PTEN (PTEN Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Phosphatase Inactive Mutant Cys124Ser

    Available Since

    Sept. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

30. pDONR-221-P3-P4

    Plasmid

    #186351

    Purpose

    Gateway donor vector with AttP3 AttP4 recombination sites

    Depositor

    Patrick Lemaire

    Article

    Roure et al PLoS One. 2007. 2(9):e916.

    Type

    Empty backbone

    Use

    Gateway donor vector

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge