We narrowed to 22,659 results for: BASE
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Plasmid#111169PurposeTet-regulated miR-E (miR-30 variant)-based RNAiDepositorInsertmiR-E (miR-30 variant)
UseRetroviralMutationWTPromoterT3GAvailable SinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pScI_dCas9-CDA
Plasmid#108549PurposeBacterial Target-AID vectorDepositorInsertdCas9-PmCDA1
TagsFlagExpressionBacterialMutationD10A and H840A for SpCas9Promoterlambda ORAvailable SinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGPTVII-Bar-U-MatryoshCaMP6s
Plasmid#100024PurposePlant expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting cpEGFP.DepositorInsertMatryoshCaMP6s
ExpressionPlantPromoterAtUBQ10Available SinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGL3 basic E2
Plasmid#48747PurposeExpression of luciferase driven by mPer2 promoter fragment containing E-box2 (bases -112 to +98 with respect to transcription start site at +1)DepositorInsertmPer2 promoter/enhancer region containing E-box2 (-112 to +98)
UseLuciferaseAvailable SinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-endo-EKAR4
Plasmid#205513PurposeGenetically encoded FRET-based biosensor for monitoring ERK kinase activity. Targeted to endosomes.DepositorInsertendo-EKAR4
Tags2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and 6xHI…ExpressionMammalianPromoterCMVAvailable SinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBWB162
Plasmid#140634PurposepBAV1k-lacI-Trc-mCherry base, lacI repression vector for ADP1DepositorInsertmCherry
ExpressionBacterialAvailable SinceSept. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMHG-W57* reporter
Plasmid#72850PurposeGFP-based reporter construct pMHG-W57* capable of detecting dose-dependent drug-induced PTC readthrough both by fluorescence microscopy and flow cytometry.DepositorInsertGFP
ExpressionMammalianAvailable SinceJan. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMB1609_pRR-EGFP
Plasmid#65852PurposeEGFP-based homologous recombination-dependent reporter for genome editing with TALENs or CRISPR/Cas9DepositorInsertsplit EGFP coding sequence
UseCRISPR and TALENExpressionMammalianPromoterSV40Available SinceAug. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBMN ltDHFR(DD)-YFP
Plasmid#47076PurposeecDHFR based destable domain function at low temperature for regulating protein stability in Caenorhabditis elegansDepositorInsertltDHFR(DD)
UseRetroviralTagsHA and YFPMutationamino acids mutations: R12H, N23S, G67S, V78A, E1…PromoterLTRAvailable SinceAug. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRL1342
Plasmid#70691PurposeRSF1010-based vector with extensive polylinker, and resistance to Cm and Em. Shuttle vector suitable for transfer to, and replication in, E. coli and Anabaena (Nostoc) sp. strain PCC 7120.DepositorTypeEmpty backboneUseBacterial shuttle vectorAvailable SinceNov. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGL3 basic E2
Plasmid#48747PurposeExpression of luciferase driven by mPer2 promoter fragment containing E-box2 (bases -112 to +98 with respect to transcription start site at +1)DepositorInsertmPer2 promoter/enhancer region containing E-box2 (-112 to +98)
UseLuciferaseAvailable SinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
NLS-PyronicSF/pCMV-myc-nuc
Plasmid#124814PurposeHighly resposive GFP-based pyruvate nanosensor with a nuclear localization signal.DepositorInsertPyronicSF
TagsNuclear Localization SignalExpressionMammalianPromoterCMVAvailable SinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-RAB-EKARev-NLS
Plasmid#118476PurposeddRFP-based single-color ERK biosensor targeted to the nucleus.DepositorInsertRAB-EKARev-NLS
Tags6xHIS, Nuclear localization signal (NLS), T7 tag …ExpressionMammalianPromoterCMVAvailable SinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pScI_dCas9-CDA
Plasmid#108549PurposeBacterial Target-AID vectorDepositorInsertdCas9-PmCDA1
TagsFlagExpressionBacterialMutationD10A and H840A for SpCas9Promoterlambda ORAvailable SinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV-syn-GCaMP6m-XC
Plasmid#118975PurposeImproved genetic encoded calcium sensor based on GCaMP6mDepositorInsertGCaMP6m-XC
UseAAVAvailable SinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAPB02
Plasmid#65628PurposeA transformation-associated recombination (TAR) based genomic capture and expression vector for antibiotic production in B. subtilisDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
LT3GECIR
Plasmid#111178PurposeTet-ON miR-E (miR-30 variant)-based RNAiDepositorInsertmiR-E (miR-30 variant)
UseLentiviralMutationWTPromoterT3GAvailable SinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTC217
Plasmid#70018PurposeCas9/sgRNA targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based on Bean Yellow Dwarf Virus; sgRNA= 1bDepositorInsertNuclease (Cas9/sgRNA) + Donor + GVR
UseCRISPRExpressionPlantAvailable SinceMarch 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
NanoLuc-PM
Plasmid#164784PurposeNanoluciferase (NanoLuc) targeted to the extracellular surface of the cell membrane for bioluminescence resonance energy transfer (BRET)-based measurement of ligand binding to unmodified receptorsDepositorInsertNanoluciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta (PDGFRB Oplophorus gracilirostris, Human)
TagsNanoLucExpressionMammalianPromoterCMVAvailable SinceMay 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
Fibronectin-human-EDB in pMAX
Plasmid#120403Purposeenables eukaryotic expression of human EDB (or EIIIB) containing fibronectin based on plasma fibronectin sequenceDepositorAvailable SinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only