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pLPC-Myc-TRF2ZNF1-8

Plasmid

#87179

Purpose

Retroviral stable expression of chimeric protein TRF2ZNF1-8

Depositor

Eros Lazzerini Denchi

Article

Li et al Science. 2017 Jan 12. pii: aah6752. doi:

Insert

chimeric protein TRF2ZNF1-8

Use

Retroviral

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 13, 2017

Availability

Academic Institutions and Nonprofits only

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pCfB2229

Plasmid

#67537

Purpose

EasyClone system-based yeast integrative vector carrying loxP-flanked CaLYS5 marker, integration into S. cerevisiae XI-5 chromosomal location, USER site for cloning, amp resistance

Depositor

Irina Borodina

Article

Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

Type

Empty backbone

Use

Cre/Lox; Integrative

Expression

Yeast

Available Since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pSicoR-Ti2

Plasmid

#59631

Purpose

Expression of shRNA against human T

Depositor

Miguel Ramalho-Santos

Article

Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

Insert

T

Use

RNAi

Expression

Mammalian

Promoter

U6

Available Since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only
pSicoR-Ti1

Plasmid

#59630

Purpose

Expression of shRNA against human T

Depositor

Miguel Ramalho-Santos

Article

Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

Insert

T

Use

RNAi

Expression

Mammalian

Promoter

U6

Available Since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only
pJA46

Plasmid

#59928

Purpose

gRNA for cleavage at rde-1(D801) locus in C elegans

Depositor

Andrew Fire

Article

Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

Insert

rde-1(D801) gRNA

Use

CRISPR

Available Since

Sept. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pJA45

Plasmid

#59927

Purpose

gRNA for cleavage at rde-1(D718) locus in C elegans

Depositor

Andrew Fire

Article

Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

Insert

rde-1(D718) gRNA

Use

CRISPR

Promoter

U6

Available Since

Sept. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pUAST-Gr64a

Plasmid

#21081

Depositor

Craig Montell

Article

Jiao et al Proc Natl Acad Sci U S A. 2007 Aug 28. 1

Insert

Gr64a (Gr64a Fly)

Expression

Insect

Available Since

April 28, 2009

Availability

Academic Institutions and Nonprofits only
pSB819-PKR-tam

Plasmid

#20036

Depositor

Harmit Malik

Article

Elde et al Nature. 2009 Jan 22;457(7228):485-9. doi

Insert

Protein kinase R

Expression

Yeast

Available Since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only
pHES942

Plasmid

#90408

Purpose

Expresses SIC1 udner control of synthetic promoter containing Zif268 binding sites.

Depositor

Hana El-Samad

Article

Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

Insert

pZ-SIC1

Expression

Yeast

Availability

Academic Institutions and Nonprofits only

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ER-mScarletI

Plasmid

#137805

Purpose

Visualization of the Endoplasmic Reticulum

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

KDEL

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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Ai9

Plasmid

#22799

Purpose

Rosa26 targeting vector, Cre-dependent tdtomato expression. See article for more colors.

Depositor

Hongkui Zeng

Article

Madisen et al Nat Neurosci. 2010 Jan . 13(1):133-40.

Insert

CAG-Floxed tdTomato

Use

Mouse Targeting

Available Since

Dec. 17, 2009

Availability

Academic Institutions and Nonprofits only

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VR122 pETv2 10His-pG-Tn5 (E54K, L372P)

Plasmid

#198467

Purpose

E. coli protein expression plasmid encoding hyperactive Tn5 transposase fused to protein G, in DH5α cloning strain.

Depositor

Viviana Risca

Article

Soroczynski et al bioRxiv 2024.07.11.602973

Insert

10His-pG-Tn5 (E54K, L372P)

Tags

10His-pG

Expression

Bacterial

Mutation

E54K, L372P

Promoter

Consensus φ10 T7 Promoter

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pDonor MCS Rosa26

Plasmid

#37200

Depositor

Charles Gersbach

Article

Perez-Pinera et al Nucleic Acids Res. 2012 Apr 1;40(8):3741

Inserts

Rosa26 left targeting arm
MCS
Rosa26 right targeting arm

Use

Genomic targeting - zinc finger

Available Since

July 19, 2012

Availability

Academic Institutions and Nonprofits only

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H2B-HT-mGold

Plasmid

#183988

Purpose

Expresses fluorescent HaloTag7 fusion protein in mammalian cells with nuclear localization.

Depositor

Pablo Rivera-Fuentes

Article

Emmert et al Nat Chem. 2023 Oct;15(10):1415-1421. doi

Insert

H2B (H2BC11 Human)

Tags

HaloTag, mGold

Expression

Mammalian

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pZac2.1 gfaABC1D-tdTomato

Plasmid

#44332

Depositor

Baljit Khakh

Article

Shigetomi et al J Gen Physiol. 2013 Apr 15.

Has Service

AAV5

Insert

tdTomato

Use

AAV; Adeno-associated virus ()

Expression

Mammalian

Available Since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_antiCD19_synNotch_Gal4VP64

Plasmid

#79125

Purpose

Lentiviral vector for constitutive expression of the anti-CD19 scFv synNotch Gal4DBDVP64 Receptor (PGK promoter)

Depositor

Wendell Lim

Article

Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

Insert

antiCD19_synNotch_Gal4VP64

Use

Lentiviral

Available Since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG-iCre

Plasmid

#89573

Purpose

Expresses iCre under pCAG

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

iCre

Use

Cre/Lox and Synthetic Biology

Tags

NLS

Expression

Mammalian

Available Since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

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pMito-iRFP670

Plasmid

#45462

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Insert

iRFP670

Tags

human cytochrome C oxidase subunit VIII

Expression

Mammalian

Mutation

T2A, S45T, V116I, D124E, M170L, K180M, D202L, I20…

Promoter

CMV

Available Since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

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pHR_pGK_LaG17_synNotch_Gal4VP64

Plasmid

#79127

Purpose

Lentiviral vector for constitutive expression of the LaG17 GFP nanobody synNotch Gal4DBDVP64 Receptor (PGK promoter)

Depositor

Wendell Lim

Article

Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

Insert

LaG17_synNotch_Gal4DBDVP64

Use

Lentiviral

Available Since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

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EGFP-Tpr

Plasmid

#35024

Depositor

Larry Gerace

Article

Frosst et al J Cell Biol. 2002 Feb 18;156(4):617-30.

Insert

Tpr (TPR Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 12, 2012

Availability

Academic Institutions and Nonprofits only

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