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pVF53

Plasmid

#229477

Purpose

expresses RssBE229C

Depositor

Alexandra Deaconescu

Publication

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

RssB (E229C)

Expression

Bacterial

Mutation

E229C

Promoter

T7

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pBD1

Plasmid

#229463

Purpose

Expresses RssB

Depositor

Alexandra Deaconescu

Publication

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

RssB

Expression

Bacterial

Promoter

T7

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pVF30

Plasmid

#229472

Purpose

Expresses RssB with R115A mutation

Depositor

Alexandra Deaconescu

Publication

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

RssB (R115A)

Expression

Bacterial

Mutation

R115A

Promoter

T7

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pVF29

Plasmid

#229473

Purpose

Expresses RssB with N256A mutation

Depositor

Alexandra Deaconescu

Publication

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

RssB (N256A)

Expression

Bacterial

Mutation

N256A

Promoter

T7

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pVF28

Plasmid

#229469

Purpose

Expresses RssB with D104A mutation

Depositor

Alexandra Deaconescu

Publication

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

RssB (D104A)

Expression

Bacterial

Mutation

D104A

Promoter

T7

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pVF31

Plasmid

#229468

Purpose

Expresses RssB with Q254A mutation

Depositor

Alexandra Deaconescu

Publication

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

RssB (Q254A)

Expression

Bacterial

Mutation

Q254A

Promoter

T7

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pVF16

Plasmid

#229465

Purpose

Expresses RssB with D58P mutation

Depositor

Alexandra Deaconescu

Publication

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

RssB (D58P)

Expression

Bacterial

Mutation

D58P

Promoter

T7

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pMS11

Plasmid

#229464

Purpose

Expresses RssB with His Tag

Depositor

Alexandra Deaconescu

Publication

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

RssB

Tags

10xHis

Expression

Bacterial

Promoter

T7

Available since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pVF14

Plasmid

#229455

Purpose

Expresses IraD with His-tag

Depositor

Alexandra Deaconescu

Publication

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

IraD

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pVF17

Plasmid

#229454

Purpose

Expresses IraM with His-tag

Depositor

Alexandra Deaconescu

Publication

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

IraM

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.SF-iGluSnFR.S72A

Plasmid

#106200

Purpose

Weak affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Publication

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-iGluSnFR.S72A

Use

AAV

Mutation

GltI: S72A

Promoter

CAG

Available since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJDS74-GG-NNN

Plasmid

#44715

Depositor

Nathan Lawson

Publication

Gupta et al Genome Res. 2013 Mar 11.

Type

Empty backbone

Tags

3X Flag and FOKI WT

Expression

Mammalian

Promoter

CMV

Available since

April 26, 2013

Availability

Academic Institutions and Nonprofits only

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pJDS70-GG-SNI

Plasmid

#44713

Depositor

Nathan Lawson

Publication

Gupta et al Genome Res. 2013 Mar 11.

Type

Empty backbone

Tags

3X Flag and FOKI WT

Expression

Mammalian

Promoter

CMV

Available since

April 12, 2013

Availability

Academic Institutions and Nonprofits only

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pJDS71-GG-SHD

Plasmid

#44714

Depositor

Nathan Lawson

Publication

Gupta et al Genome Res. 2013 Mar 11.

Type

Empty backbone

Tags

3X Flag and FOKI WT

Expression

Mammalian

Promoter

CMV

Available since

April 12, 2013

Availability

Academic Institutions and Nonprofits only

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FH1-MGT#2-mCherry_PGK-H2B-CFP

Plasmid

#164102

Purpose

Fluorescent reporter for genetic tracing of mesenchymal Glioblastoma cell-state.

Depositor

Gaetano Gargiulo

Publication

Schmitt et al Cancer Discov. 2021 Mar;11(3):754-777. d

Insert

MGT#2-mCherry

Use

Lentiviral and Synthetic Biology

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB_eCBmut

Plasmid

#164605

Purpose

Express the endocannabinoid non-responsive sensor GRAB_eCBmut in neurons

Depositor

Yulong Li

Publication

Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

Insert

Endocannabinoid non-responsive sensor GRAB_eCBmut

Use

AAV

Mutation

S383T, F177A

Promoter

hSyn

Available since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pSF11-wNIR-GECO2-T2A-HO1

Plasmid

#159606

Purpose

Codon-optimized near-infrared fluorescent calcium indicator NIR-GECO2 with codon-optimized heme-oxygenase in C.elegans expression plasmid

Depositor

Robert Campbell

Publication

Qian et al PLoS Biol. 2020 Nov 24;18(11):e3000965.

Insert

wNIR-GECO2-T2A-HO1

Expression

Worm

Promoter

tag-168

Available since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCAG-hdAsCas12a(D908A)-NLS(nucleoplasmin)-gs-3xHA-gs-VPR (RTW876)

Plasmid

#114078

Purpose

CAG promoter expression plasmid for human codon optimized AsCas12a-VPR(1.1)

Depositor

Keith Joung, Benjamin Kleinstiver

Publication

Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

Insert

human codon optimized DNase-inactive (D908A) enAsCas12a fused to VPR activation domain

Tags

SV40 NLS

Expression

Mammalian

Available since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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5XUAS IVS CsChrimson tdtomato_tr

Plasmid

#111545

Purpose

Optogenetics in Drosophila: UAS-induced expression of Chrimson tdTomato

Depositor

Vivek Jayaraman

Publication

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

CsChrimson_tdtomato_trafficked

Expression

Insect

Mutation

wt

Promoter

hsp70

Available since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3/hArf6(T27N)-HA

Plasmid

#79426

Purpose

Expresses C-terminally HA-tagged Arf6(T27N) in mammalian cells

Depositor

Kazuhisa Nakayama

Publication

Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

Insert

Arf6 (ARF6 Human)

Tags

HA

Expression

Mammalian

Mutation

changed Threonine 27 to Asparagine

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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