We narrowed to 28,008 results for: STI;

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CTL2 (M2225R)

Plasmid

#96952

Purpose

Expresses C-Terminal segment of C. elegans Piezo1 with M2225R mutation in E. coli

Depositor

Douglas Rees

Article

Kamajaya et al Structure. 2014 Oct 7;22(10):1520-7. doi

Insert

CTL2 (M222R) of PIEZO1

Tags

6x Histidine Tag

Expression

Bacterial

Promoter

T7 Promoter

Available Since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pACYC_HA-ZmSAE1_ZmSAE2(TRUNC)-FLAG

Plasmid

#91945

Purpose

Bacterial expression of maize SAE1 and truncated SAE2

Depositor

Richard Vierstra

Article

Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

Inserts

HA-ZmSAE1
ZmSAE2-FLAG

Tags

FLAG and HA

Expression

Bacterial

Mutation

Truncation of ZmSAE2 to mimic a splice variant

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag/S46X with 5'UTR

Plasmid

#87729

Purpose

Expresses S46X spastin M1 and M87 in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

Insert

SPAST (SPAST Human)

Expression

Mammalian

Mutation

S46X

Promoter

CMV

Available Since

March 8, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag/S44L with 5'UTR

Plasmid

#87727

Purpose

Expresses S44L spastin M1 and M87 in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

Insert

SPAST (SPAST Human)

Expression

Mammalian

Mutation

S44L

Promoter

pCMV

Available Since

March 8, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag/E112K with 5'UTR

Plasmid

#87723

Purpose

Expresses E112K spastin M1 and M87 in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

Insert

SPAST (SPAST Human)

Expression

Mammalian

Mutation

E112K

Promoter

CMV

Available Since

March 8, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag/L195V with 5'UTR

Plasmid

#87725

Purpose

Expresses L195V spastin M1 and M87 in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

Insert

SPAST (SPAST Human)

Expression

Mammalian

Mutation

L195V

Promoter

CMV

Available Since

March 8, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag 3A/myc-M1E112K

Plasmid

#87715

Purpose

Expresses myc-M1E112K spastin isoform in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

Insert

SPAST (SPAST Human)

Tags

Myc

Expression

Mammalian

Mutation

M1 isoform; E112K

Promoter

CMV

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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const #7-RFP

Plasmid

#81081

Purpose

constitutive promoter expressing RFP, biobrick construction

Depositor

Paul Adams, Jay Keasling

Article

Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

Insert

RFP

Use

P15a origin, constitutive promoter, kanr

Promoter

Constitutive Promoter - BBa_J23109

Available Since

Aug. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pconst #6-RFP

Plasmid

#81080

Purpose

constitutive promoter expressing RFP, biobrick construction

Depositor

Paul Adams, Jay Keasling

Article

Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

Insert

RFP

Use

P15a origin, constitutive promoter, kanr

Promoter

Constitutive Promoter - BBa_J23117

Available Since

Aug. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pBGSA FPR1 N297A

Plasmid

#66723

Purpose

Constitutive expression of FPR1 N297A in mammalian cells.

Depositor

Heini Miettinen

Article

Gripentrog et al Cell Signal. 2008 Feb;20(2):424-31. Epub

Insert

FPR1 (haplotype 3) (FPR1 Human)

Expression

Mammalian

Mutation

N297A

Promoter

SR-alpha

Available Since

June 25, 2015

Availability

Academic Institutions and Nonprofits only
pLenti CMV Blast DEST (706-1)

Plasmid

#17451

Purpose

3rd gen lentiviral Gateway destination vector, expression, CMV promoter, Blast

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral; Destination vector

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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LB901: pMVP/PB/Blast-DEST

Plasmid

#121873

Purpose

pMVP destination vector, empty PiggyBac transposon vector backbone w/ Blast selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Piggybac transposon, pmvp gateway destination vec…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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Tol2pA2-drl:APEX2-mCherry

Plasmid

#188945

Purpose

Plasmid for Tol2 transgenesis expressing APEX2 tagged to mitochondria and nuclei, and cytoplasmic mCherry, driven by the draculin promoter

Depositor

Owen Tamplin

Article

Agarwala et al Elife. 2022 Aug 9;11. pii: 64835. doi: 1

Insert

drl:mito-APEX2_p2A_APEX2-H2B_p2A_mCherry_polyA

Use

Tol2 destination vector (attr4-r3)

Available Since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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BPK1520

Plasmid

#65777

Purpose

Human expression plasmid for SpCas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sp-sgRNA

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

Insert

SpCas9 gRNA backbone, without spacer sequence

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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pCRISPRa_all-in-one

Plasmid

#183695

Purpose

Expresses CRISPRa components including dCas9-VP64, Synergistic Activation Mediators (SAM), mCherry, and gRNA expression cassette, blasticidin selective

Depositor

Edward Avezov

Article

Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

Type

Empty backbone

Use

CRISPR

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pINDUCER21-puro_Gateway-3xFLAG

Plasmid

#172981

Purpose

Gateway Destination vector for doxycycline-inducible expression of ORF

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Type

Empty backbone

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ALK5 T204D

Plasmid

#80877

Purpose

mammalian expression of constitutively active ALK5 T204D

Depositor

Aristidis Moustakas

Article

Moren et al J Biol Chem. 2005 Jun 10;280(23):22115-2

Insert

ALK5 (TGFBR1 Human)

Tags

HA

Expression

Mammalian

Mutation

T204D (constitutively active)

Promoter

CMV

Available Since

Aug. 26, 2016

Availability

Academic Institutions and Nonprofits only
pDualEX-SMN2

Plasmid

#214431

Purpose

Dual-color reporter express eGFP constitutively; tdTomato expression is contingent on the exclusion of the frame-shifting cassette exon 7

Depositor

Chaolin Zhang

Article

Recinos et al Nat Commun. 2024 May 7;15(1):3839. doi:

Insert

SMN2 EXON 7 (SMN2 Human)

Expression

Mammalian

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pDualIN-SMN2

Plasmid

#214430

Purpose

Dual-color reporter express eGFP constitutively; tdTomato expression is contingent on the inclusion of the frame-shifting cassette exon 7

Depositor

Chaolin Zhang

Article

Recinos et al Nat Commun. 2024 May 7;15(1):3839. doi:

Insert

SMN2 EXON 7 (SMN2 Human)

Expression

Mammalian

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pLeAPS-GFP-blast

Plasmid

#182230

Purpose

Lentiviral transfer plasmid for the LeAPS packaging system; encodes GFP-P2A-blasticidin transgene

Depositor

Michael Birnbaum

Article

Dobson et al Nat Methods. 2022 Apr;19(4):449-460. doi

Insert

GFP-P2A-Blasticidin

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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