We narrowed to 9,535 results for: SUB;

1. pU6-sgRNA EF1Alpha-puro-T2A-BFP.ERH1

   Plasmid

   #164800

   Purpose

   For CRISPRi knockdown of ERH by lentiviral delivery of ERH gRNA1

   Depositor

   David Bartel

   Article

   Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

   Inserts

   ERH CRISPRi gRNA1
   puro-T2A-BFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1Alpha and U6

   Available since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pU6-sgRNA EF1Alpha-puro-T2A-BFP.ERH2

   Plasmid

   #164801

   Purpose

   For CRISPRi knockdown of ERH by lentiviral delivery of ERH gRNA2

   Depositor

   David Bartel

   Article

   Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

   Inserts

   ERH CRISPRi gRNA2
   puro-T2A-BFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1Alpha and U6

   Available since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLentiCRISPRv1.mir451_KO

   Plasmid

   #164689

   Purpose

   For CRISPR knockout of miR-451 by lentiviral delivery of Cas9 and miR-451 gRNA

   Depositor

   David Bartel

   Article

   Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

   Insert

   miR-451 CRISPR KO gRNA (MIR451A )
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pK RagA(Q66L)-RagC

   Plasmid

   #99700

   Purpose

   Bacterial expression of RagA(Q66L)-RagC

   Depositor

   David Sabatini, Kuang Shen

   Article

   Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

   Inserts

   RagA (RRAGA Human)
   RagC (RRAGC Human)
   

   Use

   Tags

   His tag, Poly Arg, and SUMO

   Expression

   Bacterial

   Mutation

   L91P

   Promoter

   T7

   Available since

   Nov. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-HsSENP3(307-574)

   Plasmid

   #71481

   Purpose

   Encodes the catalytic domain of human SENP3. Suitable for in vitro transcription/translation (T7).

   Depositor

   Jorge Eduardo Azevedo

   Article

   Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

   Insert

   SUMO1/sentrin specific peptidase 3 (SENP3 Human)
   

   Use

   Tags

   Hexahistidine

   Expression

   Bacterial

   Mutation

   Deleted amino acids 1-306 (catalytic domain only)

   Promoter

   T7

   Available since

   Dec. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pJR2955

   Plasmid

   #122550

   Purpose

   Expression of a C. elegan codon optimized florescent protein (CemNeptune2.5) under the intestinal specific promoter nhx-2

   Depositor

   Gary Silverman

   Article

   Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do

   Insert

   Pnhx-2_CemNeptune2.5
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pnhx-2

   Available since

   March 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. FLAG-mBAF45b IRES GFP

   Plasmid

   #17881

   Depositor

   Jerry Crabtree

   Article

   Lessard et al Neuron. 2007 Jul 19. 55(2):201-15.

   Insert

   BAF45b (Dpf1 Mouse)
   

   Use

   Tags

   FLAG and IRES EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 13, 2008

   Availability

   Academic Institutions and Nonprofits only

8. GFP-Tac

   Plasmid

   #162489

   Purpose

   Expresses Tac (IL2 recptor alpha subunit) with GFP tag in mammalian cells

   Depositor

   Lei Lu

   Article

   Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

   Insert

   Tac (IL2RA Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. FLAG-mBAF45c IRES GFP

   Plasmid

   #17882

   Depositor

   Jerry Crabtree

   Article

   Lessard et al Neuron. 2007 Jul 19. 55(2):201-15.

   Insert

   BAF45c (Dpf3 Mouse)
   

   Use

   Tags

   FLAG and IRES EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 13, 2008

   Availability

   Academic Institutions and Nonprofits only

10. pGBT-R16

    Plasmid

    #31827

    Depositor

    Stephen Ekker

    Article

    Clark et al Nat Methods. 2011 Jun;8(6):506-15. Epub

    Insert

    mini Tol2 ITR
    

    Use

    Cre/Lox; Gene trap

    Tags

    mRFP

    Expression

    Mutation

    mRFP lacks start codon

    Promoter

    beta Actin

    Available since

    Sept. 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. optoPKAW196R

    Plasmid

    #104548

    Purpose

    Expresses Cry2-mCherry-PKA W196R in mammalian cells

    Depositor

    David Lawrence

    Article

    O'Banion et al Cell Chem Biol. 2017 Oct 25. pii: S2451-

    Insert

    Cry2-mCherry-PKA W196R
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    PKA W196R

    Promoter

    CMV

    Available since

    March 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pETDuet RagA-RagC(S75N)

    Plasmid

    #99645

    Purpose

    Bacterial expression of RagA-RagC(S75N)

    Depositor

    David Sabatini, Kuang Shen

    Article

    Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

    Inserts

    RagA (RRAGA Human)
    RagC (RRAGC Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    S75N

    Promoter

    Available since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMIR-Dsred-IRES-His Keap

    Plasmid

    #62457

    Purpose

    expresses his tagged human Keap1 protein

    Depositor

    Yimon Aye

    Article

    Parvez et al J Am Chem Soc. 2014 Dec 31.

    Insert

    Keap1 (KEAP1 Human)
    

    Use

    Tags

    HisTag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJOE5751.1

    Plasmid

    #135083

    Purpose

    pBR322-based L-rhamnose-inducible vector.

    Depositor

    Josef Altenbuchner

    Article

    Hoffmann et al FEBS J. 2012 Oct;279(20):3911-24. doi: 1

    Insert

    P-rhaP-BAD-His tag-eGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBI-CMV1-mir421-CMV2-mir1

    Plasmid

    #164673

    Purpose

    Express miR-421 and miR-1-1 in mammalian cells

    Depositor

    David Bartel

    Article

    Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

    Inserts

    miR-421 (MIR421 Human)
    miR-1-1 (MIR1-1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGMC00014

    Plasmid

    #172526

    Purpose

    non targeting sgRNA

    Depositor

    Raj Chari

    Article

    Ruf et al Cancer Immunol Res. 2021 Jun 30. pii: 23

    Insert

    Non-targeting guide
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEGFP-PSPC1-CT

    Plasmid

    #124374

    Purpose

    expresses EGFP-PSPC1-CT131 in mammalian cell

    Depositor

    Yuh-Shan Jou

    Article

    Lang et al Nat Commun. 2019 Dec 16;10(1):5716. doi:

    Insert

    PSPC1 CT (PSPC1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pICH-chl-roGFP2-GPXC71S

    Plasmid

    #191703

    Purpose

    Ti plasmid pICH86988 harbouring roGFP2-GPXC71S -a plastid-targeted control roGFP2 fusion not responsive to peroxides used alongside plasmids 191699 and 191701

    Depositor

    Philip Mullineaux

    Article

    Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

    Insert

    chl-redox(ro)GFP2-GPXC71S
    

    Use

    Binary agrobacterium ti plasmid

    Tags

    Expression

    Plant

    Mutation

    Peroxidatic Cysteine 71 replaced with Serine

    Promoter

    CaMV 35S

    Available since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAV-SYN-integrin alpha9-V5

    Plasmid

    #203729

    Purpose

    AAV vector plasmid expressing human integrin subunit alpha 9 (ITGA9) fused to V5-tag under the human synapsin (SYN) promoter

    Depositor

    Bart Nieuwenhuis, Richard Eva

    Insert

    ITGA9 (ITGA9 Human)
    

    Use

    AAV

    Tags

    V5-tag

    Expression

    Mammalian

    Mutation

    Promoter

    Human synapsin promoter

    Available since

    July 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGBW-m4046940

    Plasmid

    #149261

    Purpose

    Bacterial expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;StrepII

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    May 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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