We narrowed to 9,861 results for: SUB;

1. pHR-PD-1 G224A-mGFP

   Plasmid

   #180819

   Purpose

   Expression of human PD-1 G224A fused to mEGFP

   Depositor

   Enfu Hui

   Article

   Xu et al Elife. 2021 Nov 4;10. pii: 74276. doi: 1

   Insert

   PD-1 (PDCD1 Human)
   

   Use

   Lentiviral

   Tags

   mEGFP

   Expression

   Mutation

   G224A

   Promoter

   SFFV

   Available since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGL3_5UTR_Myc_FLuc

   Plasmid

   #229502

   Purpose

   Myc 5' untranslated region firefly luciferase reporter.

   Depositor

   Davide Ruggero

   Article

   Kovalski et al Nat Cell Biol. 2025 Feb 4. doi: 10.1038/

   Insert

   Myc 5'UTR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   Feb. 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. AAV-Flex-rev-oChIEF-tdTomato

   Plasmid

   #30541

   Purpose

   Cre-dependent viral expression of oChIEF-tdTomato for optogenetic excitation

   Depositor

   Rory McQuiston

   Article

   Bell et al Neuropharmacology. 2011 Aug 25.

   Insert

   oChIEF-tdtomato
   

   Use

   AAV and Cre/Lox

   Tags

   tdTomato

   Expression

   Mutation

   Promoter

   CAG

   Available since

   Sept. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCX-SpiCee-NES

   Plasmid

   #140901

   Purpose

   mammalian expression of SpiCee-NES, a nucleus-excluded variant of the calcium buffer SpiCee that prevents downstream signaling

   Depositor

   Xavier Nicol

   Article

   Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

   Insert

   SpiCee-NES
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   July 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3 MEKK1 KD

   Plasmid

   #12180

   Depositor

   Gary Johnson

   Article

   Widmann et al Mol Cell Biol. 1998 Apr . 18(4):2416-29.

   Insert

   MEKK1 (Map3k1 Mouse)
   

   Use

   Tags

   His and Xpress

   Expression

   Mammalian

   Mutation

   Kinase dead: K1253M; V1467L

   Promoter

   Available since

   Sept. 22, 2006

   Availability

   Academic Institutions and Nonprofits only

6. pQE60 R130A wgMDH

   Plasmid

   #204266

   Purpose

   Bacterial expression of R130A wgMDH with IPTG induction - Active site mutant

   Depositor

   Malate dehydrogenase CUREs Community (MCC)

   Article

   Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

   Insert

   MDHG_CITLA
   

   Use

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   R130A

   Promoter

   Available since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. mCherry-FKBP-PI4KAC1001

   Plasmid

   #139311

   Purpose

   Recruitable PI4KA catalytic fragment

   Depositor

   Gerry Hammond

   Article

   Zewe et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   mCherry: FKBP1A(3-108): GGSA]4GG:PI4KA(1102-2103) (PI4KA Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   G1986D

   Promoter

   Available since

   April 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3-AU1-mTOR-E2419K

   Plasmid

   #19994

   Depositor

   Fuyuhiko Tamanoi

   Article

   Urano et al Proc Natl Acad Sci U S A. 2007 Feb 27. 1

   Insert

   mammalian target of rapamycin-E2419K (Mtor Rat)
   

   Use

   Tags

   AU1

   Expression

   Mammalian

   Mutation

   Changed Glutamine 2419 to Lysine

   Promoter

   Available since

   Jan. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGBW-m4046828

   Plasmid

   #145742

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;6xHIS

   Expression

   Bacterial

   Mutation

   Escherichia coli recode 1

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pZac2.1 gfaABC1D-LifeAct-GFP

    Plasmid

    #190199

    Purpose

    Expresses the small actin binding peptide, LifeAct, with a GFP reporter

    Depositor

    Baljit Khakh

    Article

    Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

    Insert

    LifeAct-eGFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    gfaABC1D

    Available since

    April 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTS26_QUAS_CsChrimson

    Plasmid

    #175548

    Purpose

    Expresses the optogenetic channel CsChrimson off of a QUAS promoter and contains Piggybac terminal repeats for transposon-mediated integration

    Depositor

    Leslie Vosshall

    Article

    Sorrells et al Elife. 2022 May 12;11:e76663. doi: 10.75

    Insert

    CsChrimson
    

    Use

    Tags

    tdTomato

    Expression

    Insect

    Mutation

    Promoter

    QUAS

    Available since

    May 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28a-LgBiT-SpyCatcher

    Plasmid

    #223634

    Purpose

    Protein purification of SpyCatcher003 (S49C) with LgBiT and 6xHis tags from E. coli

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

    Insert

    SpyCatcher003 S49C
    

    Use

    Tags

    His6, LgBiT

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pyc126m

    Plasmid

    #158777

    Purpose

    ribosome-tagged GCaMP for soma localization

    Depositor

    Jennifer Garrison, Zachary Knight

    Article

    Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

    Insert

    GCaMP6m-RPL10a
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRT498-3xOtUBD

    Plasmid

    #190088

    Purpose

    Expresses 3 tandem OtUBD with an TEV-cleavable N-terminal 6xHis-MBP tag as well as an engineered N-terminal cysteine in E. coli

    Depositor

    Mark Hochstrasser

    Article

    Zhang et al PLoS Biol. 2022 Jun 30;20(6):e3001501. d

    Insert

    3xOtUBD
    

    Use

    Tags

    6xHis and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEGFP‐RNASEH2C

    Plasmid

    #108698

    Purpose

    For expression of N-terminally eGFP-tagged human RNASEH2C in mammalian cells

    Depositor

    Martin Reijns, Andrew Jackson

    Article

    Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6

    Insert

    RNASEH2C (RNASEH2C Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLVX-EF1a-EGFP-VAMP2-IRES-Puromycin

    Plasmid

    #134661

    Purpose

    Expresses EGFP-tagged synaptic vesicle protein

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    vesicle-associated membrane protein 2 (VAMP2 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCHC005 (∆RE)

    Plasmid

    #226199

    Purpose

    Conjugation knockout plasmid (pK18mobsacB) to delete H16_0006 (Type I restriction endonuclease subunit).

    Depositor

    Christopher Johnson

    Article

    Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

    Insert

    Homology arms for type I restriction-modification system restriction subunit H16_A0006
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 18, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pHAGE DN Cullin3

    Plasmid

    #41913

    Depositor

    Stephen Elledge

    Article

    Emanuele et al Cell. 2011 Oct 14;147(2):459-74. doi: 10

    Insert

    Cullin 3 (CUL3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pILGFPI7

    Plasmid

    #83555

    Purpose

    Used to evaluate the expression output of CUP1 promoter with yEGFP used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Insert

    CUP1 promoter-yEGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    CUP1

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAM233

    Plasmid

    #226655

    Purpose

    pcDNA3.1-p97-FLAG

    Depositor

    Andreas Martin

    Article

    Blythe et al Structure. 2019 Dec 3;27(12):1820-1829.e

    Insert

    p97 (VCP Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge