We narrowed to 11,554 results for: Vars;

1. LentiV2-hU6-evopreQ1

   Plasmid

   #210189

   Purpose

   pegRNA/ngRNA library cloning

   Depositor

   Yin Shen

   Article

   Ren et al Mol Cell. 2023 Dec 21;83(24):4633-4645.e

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. G783A_pLenti-Tet-BattP(GA)-miRFP670-2A-iCasp9-2A-Hygro_rtTA3

   Plasmid

   #200635

   Purpose

   Negative selection hygromycin resistant Tet-inducible Matreyek Bxb1(GA) landing pad for dual landing pad cells

   Depositor

   Kenneth Matreyek

   Article

   Roelle et al ACS Synth Biol. 2023 Nov 3. doi: 10.1021

   Insert

   iCasp9
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   pTRE3G

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAB082a

   Plasmid

   #79233

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   Ppro1 rpoZ-Cry1Ac-d123
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Ppro1

   Available Since

   Dec. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. Lenti-EF1alpha-VP64-dCas9-p300_Blast

   Plasmid

   #192654

   Purpose

   3rd generation lenti vector encoding VP64-dCas9-p300 with 2A Blast resistance marker

   Depositor

   Jeffrey Miner

   Article

   Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

   Insert

   VP64-dCas9-p300
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A; H840A in Cas9

   Promoter

   EF1a

   Available Since

   March 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV_hSynapsin_psychLight2

   Plasmid

   #163909

   Purpose

   To generate Adeno-Associated viruses for expression of psychLight2 under synapsin promoter

   Depositor

   Lin Tian

   Article

   Dong et al Cell. 2021 Apr 8. pii: S0092-8674(21)003

   Has Service

   AAV9

   Insert

   psychLight2
   

   Use

   AAV

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin

   Available Since

   May 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. S100A6

   Plasmid

   #196217

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses S100A6.

   Depositor

   Lalit Deshmukh

   Article

   Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

   Insert

   S100A6 (S100A6 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. Lenti-EF1alpha-VP64-dCas9-VP64_Blast

   Plasmid

   #192650

   Purpose

   3rd generation lenti vector encoding VP64-dCas9-VP64 with 2A Blast resistance marker

   Depositor

   Jeffrey Miner

   Article

   Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

   Insert

   VP64-dCas9-VP64
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A and N863A in Cas9

   Promoter

   EF1a

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV7 mitoDendra2

   Plasmid

   #157757

   Purpose

   Expression of the fluorescent protein Dendra2 targeted to mitochondria

   Depositor

   Marc Tramier

   Article

   Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

   Insert

   Dendra2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   MTS coxVIII +Dendra2

   Promoter

   CMV

   Available Since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. Lenti-EF1alpha-dCas9-10xGCN4_Hygro

   Plasmid

   #192651

   Purpose

   3rd generation lenti vector encoding dCas9-10xGCN4 (Suntag) with 2A Hygro resistance marker

   Depositor

   Jeffrey Miner

   Article

   Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

   Insert

   dCas9-10xGCN4
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A and N863A in Cas9

   Promoter

   EF1a

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. ABE8r

    Plasmid

    #208294

    Purpose

    Expresses ABE8r in mammalian cells

    Depositor

    Weixin Tang

    Article

    Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    TadA8r-SpCas9 D10A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    W23R, H36L, R47K, P48A, R51L, I76Y, V82S, A106V, …

    Promoter

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. eSrtA(2A-9) pET29b

    Plasmid

    #75145

    Purpose

    reprogrammed sortase A variant that recognizes alternative substrate LAXTG with high activity and specificity

    Depositor

    David Liu

    Article

    Dorr et al Proc Natl Acad Sci U S A. 2014 Sep 16;11

    Insert

    reprogrammed sortase A variant
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    S102C A104H E105D K138P K152I N160K K162H T164N K…

    Promoter

    T7

    Available Since

    July 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. Lenti-EF1alpha-scFv-p65-HSF1-Blast

    Plasmid

    #192652

    Purpose

    3rd generation lenti vector encoding scFV-p65-HSF1 with 2A Blast resistance marker

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    scFv-p65-HSF1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFCcGi

    Plasmid

    #59324

    Purpose

    Inducible expression of GFP and constitutive expression of mCherry

    Depositor

    Sophie Helaine, David Holden

    Article

    Figueira et al MBio. 2013 Apr 16;4(2):e00065. doi: 10.1

    Inserts

    mCherry
    EGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pbad and rpsM

    Available Since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. xCas9 3.7

    Plasmid

    #108379

    Purpose

    Mammalian expression vector for xCas9 3.7

    Depositor

    David Liu

    Article

    Hu et al Nature. 2018 Feb 28. pii: nature26155. d

    Insert

    xCas9 3.7
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    A262T, R324L, S409I, E480K, E543D, M694I, E1219V

    Promoter

    Available Since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAGGS-Bxb1o_nlsFlag

    Plasmid

    #119901

    Purpose

    Expresses Bxb1 integrase in mammalian cells

    Depositor

    Orion Weiner

    Article

    Alexander et al Elife. 2019 May 24;8. pii: 41769. doi: 1

    Insert

    Mammalian codon optimized Bxb1 integrase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Jan. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCS2-mCherry_SNAP_SNAPV_PACT_Active

    Plasmid

    #173072

    Purpose

    Active SNAP tag at the centrosome

    Depositor

    Heidi Hehnly

    Article

    Bucko et al Elife. 2019 Dec 24;8. pii: 52220. doi: 1

    Insert

    mCherry-SNAP-SNAP-Varied-PACT
    

    Use

    Tags

    mCherry-SNAP-SNAP-Varied

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94

    Available Since

    Aug. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. piggyBAC-H2B mCherry-XL (R125K)

    Plasmid

    #189926

    Purpose

    Expresses nuclear-localized mCherry-XL (R125K) in mammalian cells

    Depositor

    Ralph Jimenez

    Article

    Mukherjee et al J Phys Chem B. 2022 Jun 16. doi: 10.1021

    Insert

    mCherry-XL (R125K)
    

    Use

    Tags

    H2B

    Expression

    Mammalian

    Mutation

    differs from mCherry by R125K, W143S, I161V, Q163…

    Promoter

    CMV7

    Available Since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCas9

    Plasmid

    #167547

    Purpose

    Used for CRISPR-Cas9 mediated recombineering in Enterococcus. Can clone desired gRNA using BsaI digestion.

    Depositor

    Howard Hang

    Article

    Chen et al Appl Environ Microbiol. 2021 Jul 7:AEM00

    Inserts

    chloramphenicol acetyl transferase
    pUC19 origin of replication
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA5/FRT/TO_Beta4Gal-HaloTag7-T2A-Lyn11-SNAPf

    Plasmid

    #175524

    Purpose

    Co-expression of HaloTag7 (golgi) and SNAPf-tag (membrane inner leaflet) in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    Beta4Gal-HaloTag7 and Lyn11-SNAPf
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAGC-Lb12-P1Bb

    Plasmid

    #231387

    Purpose

    For crRNA cloning with LbCas12a vectors for genome editing in Arabidopsis

    Depositor

    Qi-Jun Chen

    Article

    Xin et al aBIOTECH. 2024 Mar 26;5(2):117-126. doi:

    Insert

    U6-29
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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