We narrowed to 11,279 results for: Vars;

1. pTPH412

   Plasmid

   #185724

   Purpose

   Directed evolution of Cas9 adenine base editors with altered PAM compatibility

   Depositor

   David Liu

   Article

   Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

   Insert

   ecTadA(8e)-gp41-8N
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   ecTadA(8e) R26G

   Promoter

   Available since

   Sept. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHBJT001

   Plasmid

   #225152

   Purpose

   High copy cloning vector for integration of C-terminal FLAG epitope tag. SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in same orientation as lac promoter.

   Depositor

   Jenny-Lee Thomassin

   Article

   Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   FLAG epitope tag (35 bp)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-flex-ReaChR-citrine

   Plasmid

   #50955

   Purpose

   flexed-ReaChR-citrine in AAV2 vector under human synapsin promoter

   Depositor

   Roger Tsien

   Article

   Lin et al Nat Neurosci. 2013 Oct;16(10):1499-508.

   Insert

   ReaChR-citrine
   

   Use

   AAV

   Tags

   citrine

   Expression

   Mutation

   Promoter

   human synapsin promoter

   Available since

   Feb. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC18/promSau/Lysostaphin

   Plasmid

   #125766

   Purpose

   Production of staphylolytic protein lysostaphin from a constitutive promoter

   Depositor

   Benedykt Wladyka

   Article

   Madry et al Mol Biotechnol. 2019 Oct;61(10):774-782.

   Insert

   lysostaphin
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   sequence corresponding to the mature form protein

   Promoter

   pemIK constitutive promoter

   Available since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. LT3REVIR

   Plasmid

   #111176

   Purpose

   Tet-ON miR-E (miR-30 variant)-based RNAi

   Depositor

   Johannes Zuber

   Article

   Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

   Insert

   miR-E (miR-30 variant)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   May 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-ar1

   Plasmid

   #225219

   Purpose

   Expression androgen receptor 1 in mammalian cells

   Depositor

   Deshou Wang

   Article

   Xiao et al Commun Biol. 2024 Sep 16;7(1):1154. doi:

   Insert

   androgen receptor 1
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. modified pcAT7-Glo1

   Plasmid

   #160996

   Purpose

   Splicing reporter backbone

   Depositor

   Brenton Graveley

   Article

   Adamson et al Genome Biol. 2018 Jun 1;19(1):71. doi: 1

   Insert

   hemoglobin subunit beta (HBB Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Repeated intron 1 with 40 nucelotide deletion

   Promoter

   CAG

   Available since

   Jan. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLKO.1P shSHMT2

   Plasmid

   #106319

   Purpose

   Express shRNA targeting SHMT2

   Depositor

   Richard Possemato

   Article

   Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

   Insert

   shRNA targeting SHMT2
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Ppst93-ANME2bHR1 MCR-NG

   Plasmid

   #192774

   Purpose

   Expresses NG-tagged ANME2bHR1 MCR in Methanococcus maripaludis

   Depositor

   William B. Whitman

   Article

   Shao et al Commun Biol. 2022 Oct 20;5(1):1113. doi:

   Insert

   Methyl coenzyme M reductase
   

   Use

   Synthetic Biology; Heterologous expression in met…

   Tags

   Flag-Strep2

   Expression

   Mutation

   Promoter

   Available since

   Dec. 7, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. Ppst93-ANME1G37 MCR-NG

    Plasmid

    #192773

    Purpose

    Expresses NG-tagged ANME1G37 MCR in Methanococcus maripaludis

    Depositor

    William B. Whitman

    Article

    Shao et al Commun Biol. 2022 Oct 20;5(1):1113. doi:

    Insert

    Methyl coenzyme M reductase
    

    Use

    Synthetic Biology; Heterologous expression in met…

    Tags

    Flag-Strep2

    Expression

    Mutation

    Promoter

    Available since

    Dec. 7, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pCMV7 mitoDendra2

    Plasmid

    #157757

    Purpose

    Expression of the fluorescent protein Dendra2 targeted to mitochondria

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Insert

    Dendra2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    MTS coxVIII +Dendra2

    Promoter

    CMV

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. LentiGuide_CjCas9-Puro

    Plasmid

    #202564

    Purpose

    hU6-BsmBI-gRNA-Puro

    Depositor

    Gerald Schwank

    Article

    Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

    Insert

    CjCas9 sgRNA cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Sept. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCherry-mNrp1

    Plasmid

    #21934

    Depositor

    Guido Serini

    Article

    Valdembri et al PLoS Biol. 2009 Jan 27. 7(1):e25.

    Insert

    neuropilin 1 (Nrp1 Mouse)
    

    Use

    Tags

    Cherry and His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

14. pCMV-Cas9-NRCH

    Plasmid

    #136926

    Purpose

    Expresses Cas9-NRCH in mammalian cells

    Depositor

    David Liu

    Article

    Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

    Insert

    Cas9-NRCH
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    see manuscript

    Promoter

    CMV

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28a(+)- PC-MjMAT

    Plasmid

    #179372

    Purpose

    PC-MjMAT is an engineered methionine adenosyltransferase that catalyses the chemo-enzymatic generation of AdoMet analogs carrying photocaging groups.

    Depositor

    Andrea Rentmeister

    Article

    Peters et al Chembiochem. 2021 Oct 4. doi: 10.1002/cb

    Insert

    PC-MjMAT
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    L147A, I351A

    Promoter

    T7

    Available since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTPH405c

    Plasmid

    #185726

    Purpose

    Directed evolution of Cas9 adenine base editors with altered PAM compatibility

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

    Insert

    gIII(N)-NpuN-NpuC-gIII(C) single PAM linker
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDAS12125_PEAR-GFP

    Plasmid

    #177178

    Purpose

    PEAR-GFP target plasmid with split EGFP and disrupted 5' splice site

    Depositor

    Ervin Welker

    Article

    Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

    Insert

    EGFP split with an intron between amino acids 95-96
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    disrupted 5' splice site

    Promoter

    CMV

    Available since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.cytGAP

    Plasmid

    #78734

    Purpose

    Expression of Ca2+ sensor GAP in the cytosol

    Depositor

    Teresa Alonso

    Article

    Rodriguez-Garcia et al Proc Natl Acad Sci U S A. 2014 Feb 18;11

    Insert

    cytGAP
    

    Use

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. J135F_BattB_Vif(T27X4)-IRES-SpyTag-sfmCherry2(11)-PuroR

    Plasmid

    #200637

    Purpose

    Recombination plasmid for Matreyek Bxb1(GT) landing pad in dual landing pad cells

    Depositor

    Kenneth Matreyek

    Article

    Roelle et al ACS Synth Biol. 2023 Nov 3. doi: 10.1021

    Insert

    Vif
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T27stop

    Promoter

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLKO.1P shACO1

    Plasmid

    #169884

    Purpose

    Suppress ACO1

    Depositor

    Richard Possemato

    Article

    Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

    Insert

    shRNA against ACO1
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge