Skip to main content
Addgene
Showing: 2941 - 2960 of 7226 results

  6. AAV-hSyn-RiboL1-jGCaMP8m

    Plasmid
    #169248
    Purpose
    AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8m, with a flexible GS-linker sequence attaching the ribosomal tag to the jGCaMP8m protein.
    Depositor
    Marianne Fyhn
    Article
    Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1
    Insert
    RiboL1-jGCaMP8m
    Use
    AAV
    Tags
    6xHis
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Available since
    Dec. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    169248-plasmid-map-sequence-id-349877

    Enlarge

  8. AAV-Syn-NS1

    Plasmid
    #175276
    Purpose
    AAV vector mediating bicistronic expression of NS1 gene of YFV-17D and dTomato with NLS
    Depositor
    Wei Xu
    Article
    Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s
    Insert
    Nonstructural protein 1 (NS1) of YFV-17D; NLS-dTomato (POLY Yellow fever virus strain 17D)
    Use
    AAV
    Tags
    NLS-dTomato (P2A cleavage)
    Expression
    Mutation
    Promoter
    Synapsin
    Available since
    Nov. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    175276-plasmid-map-sequence-id-349086

    Enlarge

  9. pET30a-His6-ABP-LOXCAT

    Plasmid
    #139971
    Purpose
    Fusion of A. viridans lactate oxidase (LOX) and E. coli catalase (CAT) that converts lactate into pyruvate without producing H2O2. Contains a albumin binding peptide (ABP).
    Depositor
    Vamsi Mootha
    Article
    Patgiri et al Nat Biotechnol. 2020 Jan 13. pii: 10.103
    Insert
    A fusion of lactate oxidase from A. viridans and catalase from E. coli
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Sept. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    139971-plasmid-map-sequence-id-311161

    Enlarge

  10. FHL-cpmTq2-Calcium-lifetime-sensor

    Plasmid
    #129628
    Purpose
    Genetically encoded calcium sensor (GECI) that reports with lifetime and intensity changes
    Depositor
    Dorus Gadella
    Article
    van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi:
    Insert
    FHL-cpmTq2-Calcium-lifetime-sensor
    Use
    Tags
    6x His - Modified TorA MNNNDLFQASRRRFLAQLG[G to …
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CMV,&Rhamnose
    Available since
    June 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    129628-plasmid-map-sequence-id-260204

    Enlarge

  11. pB80-SSPB(micro)-mVenus-GCN4-ppKin14VIb(861-1321)_P2A_iLID-mCherry-RAB11

    Plasmid
    #174645
    Purpose
    Optogenetic coupling of RAB11 to tetramerized moss kinesin-14 to induce retrograde transport of recycling endosomes
    Depositor
    Lukas Kapitein
    Article
    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133
    Insert
    SSPB(micro)-mVenus-GCN4-ppKin14VIb(861-1321)-P2A-ILID-mCherry-RAB11
    Use
    Tags
    SSPB(micro)-Venus and iLID-mCherry
    Expression
    Mammalian
    Mutation
    SSPB:Arg73Gln; mVenus: Met1Del, Thr154Met; ppKin1…
    Promoter
    Chicken beta-actin
    Available since
    Nov. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    174645-plasmid-map-sequence-id-347047

    Enlarge

  15. NanoLuc-PM-Venus

    Plasmid
    #164786
    Purpose
    Nanoluciferase (NanoLuc) targeted to the extracellular surface of the cell membrane and intracellularly labeled with monomeric Venus fluorescent protein
    Depositor
    Laszlo Hunyady
    Article
    Toth et al J Biol Chem. 2021 Feb 2:100366. doi: 10.
    Insert
    Nanoluciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta and Venus (PDGFRB Human, Oplophorus gracilirostris)
    Use
    Tags
    NanoLuc and mVenus
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    164786-plasmid-map-sequence-id-326679

    Enlarge

  16. PX458 eSp(1.1) V3

    Plasmid
    #226963
    Purpose
    CBh-eSpCas9(1.1)-2A-GFP, and hU6-sgRNA (Sp) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.
    Depositor
    Paul Thomas
    Article
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    226963-plasmid-map-sequence-id-450822

    Enlarge

  17. pcDNA5/FRT/TO-GAlphasS-RLuc8

    Plasmid
    #140980
    Purpose
    Encodes a G alpha subunit (GNAS2) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GAlphasS-RLuc8
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140980-plasmid-map-sequence-id-300680

    Enlarge

  18. AAV-TRE-NS1-dTomato

    Plasmid
    #175278
    Purpose
    AAV vector mediating inducible bicistronic expression of NS1 gene of YFV-17D and dTomato
    Depositor
    Wei Xu
    Article
    Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s
    Insert
    NS1; dTomato (POLY Yellow fever virus strain 17D)
    Use
    AAV
    Tags
    dTomato (from bidirectional promoter)
    Expression
    Mutation
    Promoter
    bidirectional TRE promoter
    Available since
    Dec. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    175278-plasmid-map-sequence-id-349702

    Enlarge

  19. AAV-hSyn-RiboL1-jGCaMP8f

    Plasmid
    #169249
    Purpose
    AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8f, with a flexible GS-linker sequence attaching the ribosomal tag to the jGCaMP8f protein.
    Depositor
    Marianne Fyhn
    Article
    Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1
    Insert
    RiboL1-jGCaMP8f
    Use
    AAV
    Tags
    6xHis
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Available since
    Dec. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    169249-plasmid-map-sequence-id-379407

    Enlarge

Showing: 2941 - 2960 of 7226 results