We narrowed to 5,405 results for: dre

1. pYX-N-Puro-mNeon-WNK3

   Plasmid

   #222674

   Purpose

   endogenously tag WNK3 with N-terminal mNeon

   Depositor

   Jason Moffat

   Article

   Xiao et al Cell Rep. 2024 Jul 23;43(7):114417. doi:

   Insert

   WNK3 (WNK3 Human)
   

   Use

   Tags

   mNeon

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDNA5_miniTurbo-C12orf49

   Plasmid

   #155111

   Purpose

   transfection plasmid for the exogneous expression of N-terminal miniTurbo-tagged C12orf49 for generation of FlpIn cell lines for BioID

   Depositor

   Jason Moffat

   Article

   Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

   Insert

   C12orf49 (SPRING1 Human)
   

   Use

   Tags

   miniTurbo

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/TO inducible promoter

   Available since

   Aug. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. sgTP53(A)

   Plasmid

   #85559

   Purpose

   Expresses sgRNA targeting human TP53 around codon F109

   Depositor

   Luca Grumolato

   Article

   Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

   Insert

   tumor protein p53 (TP53 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

4. pTT3-ecdICAM1-ST3-His

   Plasmid

   #210666

   Purpose

   Expression of the extracellular domain of human ICAM1 fused to Spytag003 + Histag in HEK293 (or similar)

   Depositor

   Omer Dushek

   Article

   Patel et al EMBO J. 2024 Jan;43(1):132-150. doi: 10.

   Insert

   Extracellular domain of human ICAM-1 fused to Spytag003 and Histag (ICAM1 Human, Synthetic)
   

   Use

   Tags

   Histag and Spytag003

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-Halo-Tag-128-425-Akap9

   Plasmid

   #196873

   Purpose

   Expression of fragment 128-425 from A-Kinase Anchoring Protein 9 (Akap9) fused to Halo-Tag. Used to displace endogenous Akap9 from Golgi

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   Halo-Tag-Akap9 (128-425) (Akap9 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Quimeric CAG

   Available since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pnEA-vH_His-TEV-SEPT2-sfCherry2_SEPT6

   Plasmid

   #180313

   Purpose

   bacterial co-expression of human SEPT2 fused to superfolder Cherry2 and of human SEPT6

   Depositor

   Manos Mavrakis

   Article

   Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

   Inserts

   SEPTIN2 (SEPTIN2 Human)
   SEPTIN6 (SEPTIN6 Human)
   

   Use

   Tags

   His6-TEV and sfCherry2

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. TH0901-pOCC177-FUS-wt_opt(Nhe)

   Plasmid

   #221885

   Purpose

   Shuttle vector for baculovirus production, using FlashBac bacmid

   Depositor

   Anthony Hyman

   Article

   Wang et al Cell. 2018 Jul 26;174(3):688-699.e16. do

   Insert

   FUS (FUS Human)
   

   Use

   Tags

   6xHis-MBP-PreScission and TEV-SNAP-tag

   Expression

   Insect

   Mutation

   wt

   Promoter

   PH promoter

   Available since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. Lenti-sgPten#2/Cre

   Plasmid

   #173646

   Purpose

   Expresses a Pten-targeting gRNA and Cre-recombinase

   Depositor

   Charles Swanton

   Article

   Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

   Insert

   gRNA targeting Pten (Pten Mouse)
   

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA5_C12orf49-miniTurbo

   Plasmid

   #155112

   Purpose

   transfection plasmid for the exogneous expression of C-terminal miniTurbo-tagged C12orf49 for generation of FlpIn cell lines for BioID

   Depositor

   Jason Moffat

   Article

   Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

   Insert

   C12orf49 (SPRING1 Human)
   

   Use

   Tags

   3x Flag and miniTurbo

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/TO inducible promoter

   Available since

   Aug. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. JDW 743 (pDONR221-EYFP-hKRAS4B-WT)

    Plasmid

    #156402

    Purpose

    Gateway middle entry clone encoding EYFP-fused to human KRAS4B, includes a stop codon

    Depositor

    Joshua Wythe

    Article

    Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

    Insert

    human wild type KRAS4B-WT (KRAS Human)
    

    Use

    Gateway cloning

    Tags

    EYFP

    Expression

    Mutation

    Promoter

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBactin-td-mCherry-Cdk5rap2-CTD

    Plasmid

    #196854

    Purpose

    Expression of the centrosome targeting carboxy-terminal domain (CTD) of Cdk5rap2 fused to tandem (td)-mCherry. Used to displace endogenous Cdk5rap2 from centrosomes.

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    td-mCherry-Cdk5rap2-CTD (Cdk5rap2 Discosoma sp.)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None (wt)

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLgw ELK4-V5-EcoDam

    Plasmid

    #98607

    Purpose

    Mammalian DamID lentiviral vector for ELK4 with Dam-V5 using Gateway cloning

    Depositor

    Richard Harvey

    Article

    Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

    Insert

    ELK4 (Elk4 Mouse)
    

    Use

    Lentiviral; Damid

    Tags

    V5 and Dam (DNA adenine methyltransferase)

    Expression

    Mammalian

    Mutation

    Promoter

    Heat Shock Minimal Promoter

    Available since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYX-N-Puro-mNeon-TSC22D4

    Plasmid

    #222671

    Purpose

    endogenously tag TSC22D4 with N-terminal mNeon

    Depositor

    Jason Moffat

    Article

    Xiao et al Cell Rep. 2024 Jul 23;43(7):114417. doi:

    Insert

    TSC22D4 (TSC22D4 Human)
    

    Use

    Tags

    mNeon

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCW57.1 KBTBD4 R313PRR

    Plasmid

    #184626

    Purpose

    Doxycycline inducible lentiviral vector for N-terminal Flag tagged KBTBD4 R313PRR expression in mammalian cells

    Depositor

    Vincenzo D'Angiolella

    Article

    Chen et al Cell Death Differ. 2022 Apr 4. pii: 10.1

    Insert

    N-terminal Flag tagged KBTBD4 R313PRR (KBTBD4 Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    Expression

    Mammalian

    Mutation

    R313PRR

    Promoter

    TRE promoter, Tet ON

    Available since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. R620-M13-303: CMV51p> SARS-CoV-2 S(1-1213)-2P-T4f-His6 B.1.1.7

    Plasmid

    #170197

    Purpose

    Mammalian expression of SARS-CoV-2 soluble spike trimer protein (B.1.1.7 variant)

    Depositor

    Dominic Esposito

    Article

    Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

    Insert

    SARS-CoV-2 S (spike) B.1.1.7 (S SARS-CoV-2)
    

    Use

    Tags

    His6

    Expression

    Mammalian

    Mutation

    Δ(69-70), Δ144, N501Y, A570D, D614G, P681H, T716I…

    Promoter

    CMV51

    Available since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. sgTP53(B)

    Plasmid

    #85560

    Purpose

    Expresses sgRNA targeting human TP53 around codon F109

    Depositor

    Luca Grumolato

    Article

    Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

    Insert

    tumor protein p53 (TP53 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

17. pRSITEP-puro-shWRN1

    Plasmid

    #125783

    Purpose

    DOX-inducible expression of a short-hairpin RNA targeting human WRN

    Depositor

    Francisca Vazquez

    Article

    Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

    Insert

    shWRN1 (WRN Human)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLKO.1-puro-shRFP

    Plasmid

    #125778

    Purpose

    constitutive expression of a short-hairpin RNA targeting RFP

    Depositor

    Francisca Vazquez

    Article

    Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

    Insert

    shRFP
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. LeGO-iG-CSF2RB(A455D)

    Plasmid

    #125751

    Purpose

    Lentiviral Expression Vektor with CSF2RB-A455D mutant cDNA and IRES eGFP

    Depositor

    Dirk Heckl

    Article

    Labuhn et al Cancer Cell. 2019 Aug 12;36(2):123-138.e

    Insert

    CSF2RB(A455D)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    A455D, codon optimized

    Promoter

    SFFV

    Available since

    Sept. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1-kappa-myc-dL5-2XG4S-mCer3-KDEL

    Plasmid

    #73209

    Purpose

    Expresses myc-dL5(E52D)-mCer3 fusion protein in endoplasmic reticulum of mammalian cells with an Igk-leader. (MBIC5, dL5**, FAP)

    Depositor

    Marcel Bruchez

    Article

    Telmer et al ACS Chem Biol. 2015 May 15;10(5):1239-46

    Insert

    kappa-myc-dL5-2XG4S-mCer3-KDEL (MYC Human, Synthetic)
    

    Use

    Tags

    The FAP and mCerulean3 are fused with 2 copies of…

    Expression

    Mammalian

    Mutation

    The dL5** FAP is E50D, L89S, also known as E52D/L…

    Promoter

    CMV

    Available since

    March 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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