We narrowed to 4,214 results for: bre
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Plasmid#136332PurposeLentiviral expression of human ECT2_T327DDepositorInsertECT2 (ECT2 Human)
UseLentiviralTagsExpressionMammalianMutationT327DPromoterAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28B-PU.1-ETS-Wing
Plasmid#124552PurposeChimeric ETS domain: Wing of Ets-1 in PU.1 scaffoldDepositorUseTags6xHis tagExpressionBacterialMutationThe 5 residues making up the "wing" in …PromoterAvailable sinceJune 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
CIRTS-FTO-control
Plasmid#216859PurposeCIRTS RNA targeting system for targeted removal of m6A on transcript at the synapse. CIRTS-FTO-Calm3 and scramble gRNA is expressed under human Syn1 and U6 promoter, respectively.DepositorInsertCIRTS-FTO-Calm3-U6-control gRNA
UseLentiviralTagsmCherry2ExpressionMammalianMutationPromoterSyn1, U6Available sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
Anti-GABA(A)R, Epsilon [N413/67R]
Plasmid#114478PurposeMammalian Expression Plasmid of anti-GABA(A)R, Epsilon (Human). Derived from hybridoma N413/67.DepositorInsertanti-GABA(A)R, Epsilon (Homo sapiens) recombinant mouse monoclonal antibody (GABRE Mouse)
UseTagsExpressionMammalianMutationPromoterdual CMVAvailable sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
CIRTS-YTHDF2-control
Plasmid#216857PurposeCIRTS RNA targeting system for targeted knockdown of m6A-containing transcript at the synapse. CIRTS-YTHDF2-Calm3 and scramble gRNA is expressed under human Syn1 and U6 promoter, respectively.DepositorInsertCIRTS-YTHDF2-Calm3-U6-control gRNA
UseLentiviralTagsGFPExpressionMammalianMutationPromoterSyn1, U6Available sinceOct. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCag FlpO-2A-Cre EV
Plasmid#129419Purposeepisomal expression of FlpO and Cre recombinasesDepositorInsertFlpO-2A-Cre
UseCre/Lox and Unspecified; Episomal expression vect…TagsExpressionMammalianMutationPromoterCMV/Chick β-actin (CAG)Available sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST-V5_Hu_p115-RhoGEF(WT)
Plasmid#136335PurposeMammalian expression of human p115-RhoGEF with N-terminal V5DepositorInsertARHGEF1 (ARHGEF1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST-EGFP_Hu_p115-RhoGEF(WT)
Plasmid#136337PurposeMammalian expression of human p115-RhoGEF with N-terminal EGFPDepositorInsertARHGEF1 (ARHGEF1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGfa2(ABDx3)-nLac
Plasmid#53133Purposeexpression of nuclear LacZ driven by Gfa2 promoter with 3 additional copies of the gfa28 ABD segment; has about 100 times higher activity than pGfa2-nLac in transfected cellsDepositorInsertGfa2 (GFAP Human)
UseMouse TargetingTagsLacZ, NLS, and mP1ExpressionMammalianMutation-2163 to +47, with 3 copies of 3 copies of the gf…Promoterhuman Gfa2Available sinceJuly 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGfa(B3)-nLac
Plasmid#53132Purposeexpression of nuclear LacZ driven by Gfa2 promoter with 3 additional B sites, has about 75 times higher activity than pGfa2-nLac in transfected cellsDepositorInsertGfa2 (GFAP Human)
UseMouse TargetingTagsLacZ, NLS, and mP1ExpressionMammalianMutation-2163 to +47, with 3 copies of the gfa2 B segment…Promoterhuman Gfa2Available sinceJuly 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
CIRTS-YTHDF2-Malat1
Plasmid#216858PurposeCIRTS RNA targeting system for targeted knockdown of m6A-containing Malat1 at the synapse. CIRTS-YTHDF2-Calm3 and Malat1 gRNA is expressed under human Syn1 and U6 promoter, respectively.DepositorInsertCIRTS-YTHDF2-Calm3-U6-Malat1 gRNA
UseLentiviralTagsGFPExpressionMammalianMutationPromoterSyn1, U6Available sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
CIRTS-FTO-Malat1
Plasmid#216860PurposeCIRTS RNA targeting system for targeted removal of m6A on Malat1 at the synapse. CIRTS-FTO-Calm3 and Malat1 gRNA is expressed under human Syn1 and U6 promoter, respectively.DepositorInsertCIRTS-FTO-Calm3-U6-Malat1 gRNA
UseLentiviralTagsExpressionMammalianMutationPromoterSyn1, U6Available sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
A1-LCD-30G+30S-12F+12Y
Plasmid#178687PurposeBacterial expression of N-terminally 6His tagged A1-LCD with thirty Gly residues replaced with Ser, twelve Phe residues replaced with Tyr (LCD: low-complexity domain, aa 186-320)DepositorInsertA1-LCD-30G+30S-12F+12Y (HNRNPA1 Human)
UseTags6xHisExpressionBacterialMutationthirty Gly residues replaced with Ser, twelve Phe…PromoterAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
A1-LCD-30G+30S+7F-7Y
Plasmid#178686PurposeBacterial expression of N-terminally 6His tagged A1-LCD with thirty Gly residues replaced with Ser, seven Tyr residues replaced with Phe (LCD: low-complexity domain, aa 186-320)DepositorInsertA1-LCD-30G+30S+7F-7Y (HNRNPA1 Human)
UseTags6xHisExpressionBacterialMutationthirty Gly residues replaced with Ser, seven Tyr …PromoterAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
A1-LCD-20G+20S+7F-7Y
Plasmid#178688PurposeBacterial expression of N-terminally 6His tagged A1-LCD with twenty Gly residues replaced with Ser, seven Tyr residues replaced with Phe (LCD: low-complexity domain, aa 186-320)DepositorInsertA1-LCD-20G+20S+7F-7Y (HNRNPA1 Human)
UseTags6xHisExpressionBacterialMutationtwenty Gly residues replaced with Ser, seven Tyr …PromoterAvailable sinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
A1-LCD-14N-4Q+18G
Plasmid#178694PurposeBacterial expression of N-terminally 6His tagged A1-LCD with fourteen Gln residues removed, four Asn removed, replaced with eighteen Gly (LCD: low-complexity domain, aa 186-320)DepositorInsertA1-LCD-14N-4Q+18G (HNRNPA1 Human)
UseTags6xHisExpressionBacterialMutationfourteen Gln residues removed, four Asn removed, …PromoterAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
A1-LCD-20G+20S-12F+12Y
Plasmid#178689PurposeBacterial expression of N-terminally 6His tagged A1-LCD with twenty Gly residues replaced with Ser, twelve Phe residues replaced with Tyr (LCD: low-complexity domain, aa 186-320)DepositorInsertA1-LCD-20G+20S-12F+12Y (HNRNPA1 Human)
UseTags6xHisExpressionBacterialMutationtwenty Gly residues replaced with Ser, twelve Phe…PromoterAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
A1-LCD+23G-23S-12F+12Y
Plasmid#178691PurposeBacterial expression of N-terminally 6His tagged A1-LCD with twenty-three Ser residues replaced with Gly, twelve Phe residues replaced with Tyr (LCD: low-complexity domain, aa 186-320)DepositorInsertA1-LCD+23G-23S-12F+12Y (HNRNPA1 Human)
UseTags6xHisExpressionBacterialMutationtwenty-three Ser residues replaced with Gly, twel…PromoterAvailable sinceMarch 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
A1-LCD+23G-23S+7F-7Y
Plasmid#178690PurposeBacterial expression of N-terminally 6His tagged A1-LCD with twenty-three Ser residues replaced with Gly, seven Tyr replaced with Phe (LCD: low-complexity domain, aa 186-320)DepositorInsertA1-LCD+23G-23S+7F-7Y (HNRNPA1 Human)
UseTags6xHisExpressionBacterialMutationtwenty-three Ser residues replaced with Gly, seve…PromoterAvailable sinceMarch 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
Gq-CASE
Plasmid#168125PurposeEncodes a BRET-based activity sensor for the heterotrimeric G protein Gq. Composed of the subunits G alpha q (GNAQ) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).DepositorUseLuciferaseTagscpVenus on GNG9ExpressionMammalianMutationNLuc is inserted at F124/E125 within GNAQPromoterAvailable sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
EFSp-GFP-YAP
Plasmid#174168PurposeLentiviral vector to constitutively express YAP under control of the EFS promoter.DepositorInsertYAP (YAP1 Human)
UseLentiviralTags3x FLAG tagExpressionMammalianMutationPromoterEFSAvailable sinceAug. 19, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
G15-CASE
Plasmid#168126PurposeEncodes a BRET-based activity sensor for the heterotrimeric G protein G15. Composed of the subunits G alpha 15 (GNA15) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).DepositorUseLuciferaseTagscpVenus on GNG9ExpressionMammalianMutationNLuc is inserted at E244/N245 within GNA15PromoterAvailable sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
Gi2-CASE
Plasmid#168121PurposeEncodes a BRET-based activity sensor for the heterotrimeric G protein Gi2. Composed of the subunits G alpha i2 (GNAI2) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).DepositorUseLuciferaseTagscpVenus on GNG2ExpressionMammalianMutationNLuc is inserted at C112/E115 within GNAI2PromoterAvailable sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
EFSp-GFP-YAP (S94A)
Plasmid#174169PurposeLentiviral vector to constitutively express TEAD-binding mutant YAP under control of the EFS promoter.DepositorInsertYAP (YAP1 Human)
UseLentiviralTags3x FLAG tagExpressionMammalianMutationSerine 94 mutated to AlaninePromoterEFSAvailable sinceAug. 19, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
PGKp-GFP-YAP
Plasmid#174172PurposeLentiviral vector to constitutively express YAP under control of the human PGK promoter.DepositorInsertYAP (YAP1 Human)
UseLentiviralTags3x FLAG tagExpressionMammalianMutationPromoterPGKAvailable sinceAug. 19, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
tetO-ASCL1-LMX1b-NURR1-PuroR
Plasmid#182298PurposeDoxycycline-inducible vector expressing the ALN transcription factors and PuroR (all separated by 2A sequences)DepositorUseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
G13-CASE
Plasmid#168127PurposeEncodes a BRET-based activity sensor for the heterotrimeric G protein G13. Composed of the subunits G alpha 13 (GNA13) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).DepositorUseLuciferaseTagscpVenus on GNG9ExpressionMammalianMutationNLuc is inserted at F125/D126 within GNA13PromoterAvailable sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
Gi1-CASE
Plasmid#168120PurposeEncodes a BRET-based activity sensor for the heterotrimeric G protein Gi1. Composed of the subunits G alpha i1 (GNAI1) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).DepositorUseLuciferaseTagscpVenus on GNG2ExpressionMammalianMutationNLuc is inserted at A121/E122 within GNAI1PromoterAvailable sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
Gi3-CASE
Plasmid#168122PurposeEncodes a BRET-based activity sensor for the heterotrimeric G protein Gi3. Composed of the subunits G alpha i3 (GNAI3) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).DepositorUseLuciferaseTagscpVenus on GNG2ExpressionMammalianMutationNLuc is inserted at A114/E115 within GNAI1PromoterAvailable sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
PGKp-GFP-TAZ
Plasmid#174176PurposeLentiviral vector to constitutively express TAZ/WWTR1 under control of the human PGK promoter.DepositorInsertTAZ (WWTR1 Human)
UseLentiviralTagsMyc tagExpressionMammalianMutationPromoterPGKAvailable sinceAug. 19, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCag SE (Self-excising) FlpO-2A-Cre EV
Plasmid#130986Purposeepisomal expression of FlpO and Cre recombinases, self excisingDepositorInsertFlpO-2A-Cre
UseCre/Lox and Unspecified; Episomal expression vect…TagsExpressionMammalianMutationprotamine intron added to FlpO to prevent bacteri…PromoterCMV/Chick β-actin (CAG)Available sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
PGKp-GFP-YAP (S94A)
Plasmid#174173PurposeLentiviral vector to constitutively express TEAD-binding mutant YAP under control of the human PGK promoter.DepositorInsertYAP (YAP1 Human)
UseLentiviralTags3x FLAG tagExpressionMammalianMutationSerine 94 mutated to AlaninePromoterPGKAvailable sinceAug. 19, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
EFSp-GFP-YAP (5SA)
Plasmid#174170PurposeLentiviral vector to constitutively express overactive YAP (5 LATS phosporylation sites mutated to Ala) under control of the EFS promoter.DepositorInsertYAP (YAP1 Human)
UseLentiviralTags3x FLAG tagExpressionMammalianMutationFive LATS phosphorylation sites (S61, S109, S127,…PromoterEFSAvailable sinceSept. 28, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
PGKp-GFP-YAP (5SA)
Plasmid#174174PurposeLentiviral vector to constitutively express overactive YAP (5 LATS phosporylation sites mutated to Ala) under control of the human PGK promoter.DepositorInsertYAP (YAP1 Human)
UseLentiviralTags3x FLAG tagExpressionMammalianMutationFive LATS phosphorylation sites (S61, S109, S127,…PromoterPGKAvailable sinceAug. 19, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
Go1-CASE
Plasmid#168123PurposeEncodes a BRET-based activity sensor for the heterotrimeric G protein Go1. Composed of the subunits G alpha o1 (GNAO1) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).DepositorUseLuciferaseTagscpVenus on GNG9ExpressionMammalianMutationNLuc is inserted at L91/G92 within GNAO1PromoterAvailable sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
Gs(short)-CASE
Plasmid#168124PurposeEncodes a BRET-based activity sensor for the heterotrimeric G protein Gs. Composed of the subunits G alpha s(short isoform) (GNAS) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 1 (GNG1).DepositorUseLuciferaseTagscpVenus on GNG1ExpressionMammalianMutationNLuc is inserted at N136/V137 within GNASPromoterAvailable sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
MADR pDonor smFP-HA (dark) WPRE
Plasmid#131007PurposeMADR donor plasmid for FlpO+Cre- mediated insertion of SM_FP-HA, a cytoplasmic fluorescent protein which includes multiple HA epitope tagsDepositorInsertsmFP-HA WPRE
UseCre/Lox and Synthetic Biology; Mosaic analysis fo…TagsHA - 10 total HA epitope tagesExpressionMammalianMutation"dark" variant with GGG fluorphore comp…Promoternone (4x polyA to mitigate episomal expression)Available sinceJuly 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
MADR pDonor smFP-V5 (dark) WPRE
Plasmid#131006PurposeMADR donor plasmid for FlpO+Cre- mediated insertion of a SM_FP-V5, a cytoplasmic fluorescent protein which includes multiple V5 epitope tagsDepositorInsertsmFP-V5 WPRE
UseCre/Lox and Synthetic Biology; Mosaic analysis fo…TagsV5 - 10 total of V5 epitope tagExpressionMutation"dark" variant with GGG fluorphore comp…Promoternone (4x polyA to mitigate episomal expression)Available sinceJuly 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
MADR pDonor smFP-Myc (dark) WPRE
Plasmid#130987PurposeMADR donor plasmid for FlpO+Cre- mediated insertion of a SM_FP-Myc, a cytoplasmic fluorescent protein which includes multiple Myc epitope tagsDepositorInsertsmFP-Myc WPRE
UseMosaic analysis for dual recombinase-mediated cas…TagsMyc - 10 total Myc tagsExpressionMutation"dark" variant with GGG fluorophore ver…Promoternone (4x polyA to mitigate episomal expression)Available sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
MADR pDonor smFP-FLAG (dark) WPRE
Plasmid#131005PurposeMADR donor plasmid for FlpO+Cre- mediated insertion of a SM_FP-FLAG, a cytoplasmic fluorescent protein which includes multiple FLAG epitope tagsDepositorInsertsmFP-FLAG WPRE
UseMosaic analysis for dual recombinase-mediated cas…TagsFLAG - 10 total FLAG tagsExpressionMutation"dark" variant with GGG fluorphore comp…Promoternone (4x polyA to mitigate episomal expression)Available sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
Split 'n coexpress CUL3-RBX1
Plasmid#52293Purposebacterial co-expression of N-terminal Cul3, C-terminal Cul3, and Rbx1DepositorUseTagsGST, IRES, and TEVExpressionBacterialMutationcontains aa 1-309, contains aa 310-768, and conta…PromoterIRES and tacAvailable sinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
PB-TAC-ERP2-(N-HA)KRAS_G12D
Plasmid#201254PurposeDox-inducible overexpression of HA-tagged KRASG12D and mCherry in human cellsDepositorInsertKRAS (KRAS Human)
UsePiggybac transposonTagsHA-TagExpressionMammalianMutationchanged Gly (G) 12 to Asp (D)PromoterTREAvailable sinceSept. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
Stag-ObMT
Plasmid#215161PurposeShanbhag lab plasmidsDepositorInsertStag-ObMT
UseTagsS-tagExpressionBacterialMutationPromoterT7 lacAvailable sinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTS_TXS
Plasmid#163839PurposeiPTG inducible expression of taxadiene synthase and geranylgeranyl diphosphate synthaseDepositorInserttaxadiene synthase (61-862)
UseTagsExpressionBacterialMutationPromoterTrcAvailable sinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBIR-GFP
Plasmid#49807PurposeIn vivo GFP based system to measure break induced replication (BIR) repair efficiency. expression of I-SceI generates a double strand break that when repaired by BIR mechanism will restore GFP.DepositorInsertGFP
UseTagsExpressionMammalianMutationPromoterbeta-actinAvailable sinceJan. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCBASceI
Plasmid#26477PurposeI-SceI endonuclease expression vector with mammalian promoter to introduce a DSB at a genomic I-SceI siteDepositorInsertpCBASceI
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceOct. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDSG594
Plasmid#214221Purposecompetition strain 10 integrase plasmidDepositorInsertPhlF, A118
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
R26P-SA-EGFP-puro
Plasmid#26890DepositorInsertEGFP
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceDec. 8, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDSG578
Plasmid#214215Purposeintegrase plasmidDepositorInsertPhlF, A118
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pC.HA
Plasmid#15126DepositorTypeEmpty backboneUseTagsHAExpressionMammalianMutationPromoterAvailable sinceJune 8, 2007AvailabilityAcademic Institutions and Nonprofits only