We narrowed to 302 results for: FRU

1. pBIG2abc_nsp12-His6-3xFlag/nsp7/nsp8 (SARS-CoV-2)

   Plasmid

   #169185

   Purpose

   Baculoviral transfer vector to co-express nsp12-His6-3xFlag, nsp7 and nsp8 (SARS-CoV-2) in insect cells

   Depositor

   John Diffley

   Article

   Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

   Insert

   nsp12-His6-3xFlag/nsp7/nsp8 (ORF1ab SARS-CoV-2, Synthetic)
   

   Use

   Tags

   His6-3xFlag (nsp12 C-terminus)

   Expression

   Insect

   Mutation

   Codon optimised for insect cells (Spodoptera frug…

   Promoter

   Polyhedrin

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBIG1b_nsp7-His6-nsp8 (SARS-CoV-2)

   Plasmid

   #169180

   Purpose

   Baculoviral transfer vector to express nsp7-His6-nsp8 fusion protein (SARS-CoV-2) in insect cells

   Depositor

   John Diffley

   Article

   Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

   Insert

   nsp7-His6-nsp8 (ORF1ab SARS-CoV-2, Synthetic)
   

   Use

   Tags

   His6 (internal tag linking nsp7 and nsp8): nsp7-H…

   Expression

   Insect

   Mutation

   Codon optimised for insect cells (Spodoptera frug…

   Promoter

   Polyhedrin

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBIG2abc_nsp12-His6-3xFlag/nsp7-Linker-nsp8 (SARS-CoV-2)

   Plasmid

   #169184

   Purpose

   Baculoviral transfer vector to co-express nsp12-His6-3xFlag and nsp7-Linker-nsp8 fusion in insect cells

   Depositor

   John Diffley

   Article

   Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

   Insert

   nsp12-His6-3xFlag/nsp7-Linker-nsp8 (ORF1ab SARS-CoV-2, Synthetic)
   

   Use

   Tags

   His6-3xFlag (nsp12 C-terminus) and nsp7-nsp8 fusi…

   Expression

   Insect

   Mutation

   Codon optimised for insect cells (Spodoptera frug…

   Promoter

   Polyhedrin

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBIG2abc_nsp12-3xFlag/nsp7-His6-nsp8 (SARS-CoV-2)

   Plasmid

   #169183

   Purpose

   Baculoviral transfer vector to co-express nsp12-3xFlag and nsp7-His6-nsp8 fusion in insect cells

   Depositor

   John Diffley

   Article

   Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

   Insert

   nsp12-3xFlag/nsp7-His6-nsp8 (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   3xFlag (nsp12 C-terminus) and His6 (as internal t…

   Expression

   Insect

   Mutation

   Codon optimised for insect cells (Spodoptera frug…

   Promoter

   Polyhedrin

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBIG2ab_nsp14/nsp10-6His-3xFlag (SARS-CoV-2)

   Plasmid

   #169164

   Purpose

   Baculoviral transfer vector to co-express SARS-CoV-2 nsp14 and nsp10 in insect cells

   Depositor

   John Diffley

   Article

   Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

   Insert

   nsp14/nsp10-6His-3xFlag (ORF1ab SARS-CoV-2, Synthetic)
   

   Use

   Tags

   6His-3xFlag (nsp10 C-terminus)

   Expression

   Insect

   Mutation

   Codon optimised for insect cells (Spodoptera frug…

   Promoter

   Polyhedrin

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBIG1b_nsp7-Linker-nsp8 (SARS-CoV-2)

   Plasmid

   #169181

   Purpose

   Baculoviral transfer vector to express untagged nsp7-Linker-nsp8 fusion protein (SARS-CoV-2) in insect cells

   Depositor

   John Diffley

   Article

   Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

   Insert

   nsp7-Linker-nsp8 (ORF1ab SARS-CoV-2, Synthetic)
   

   Use

   Tags

   No purification tag and nsp7-nsp8 fusion protein …

   Expression

   Insect

   Mutation

   Codon optimised for insect cells (Spodoptera frug…

   Promoter

   Polyhedrin

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBIG1b_nsp10-6His-3xFlag (SARS-CoV-2)

   Plasmid

   #169162

   Purpose

   Baculoviral transfer vector to express SARS-CoV-2 nsp10 in insect cells

   Depositor

   John Diffley

   Article

   Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

   Insert

   nsp10-6His-3xFlag (ORF1ab SARS-CoV-2, Synthetic)
   

   Use

   Tags

   6His-3xFlag

   Expression

   Insect

   Mutation

   Codon optimised for insect cells (Spodoptera frug…

   Promoter

   Polyhedrin

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBIG1a_3xFlag-6His-nsp14 (SARS-CoV-2)

   Plasmid

   #169163

   Purpose

   Baculoviral transfer vector to express SARS-CoV-2 nsp14 in insect cells

   Depositor

   John Diffley

   Article

   Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

   Insert

   3xFlag-6His-nsp14 (ORF1ab SARS-CoV-2, Synthetic)
   

   Use

   Tags

   3xFlag-6His

   Expression

   Insect

   Mutation

   Codon optimised for insect cells (Spodoptera frug…

   Promoter

   Polyhedrin

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDONR223-KHK

   Plasmid

   #23586

   Depositor

   David Root, William Hahn

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   KHK (KHK Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

10. pDONR223-FN3K

    Plasmid

    #23621

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    FN3K (FN3K Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

11. pDONR223-FN3KRP

    Plasmid

    #23679

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    FN3KRP (FN3KRP Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

12. pLIB_3xFlag-His-nsp5CS-nsp9 (SARS-CoV-2)

    Plasmid

    #169177

    Purpose

    Baculoviral transfer vector to express SARS-CoV-2 nsp9 with nsp5-cleavable 3xFlag-His6 tag in insect cells

    Depositor

    John Diffley

    Article

    Milligan et al Biochem J. 2021 Jul 16;478(13):2499-2515

    Insert

    3xFlag-His-nsp5CS-nsp9 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    3xFlag-His6 (nsp5-cleavable)

    Expression

    Insect

    Mutation

    Codon optimised for insect cells (Spodoptera frug…

    Promoter

    Polyhedrin

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLIB_3xFlag-His-PLpro (nsp3) (SARS-CoV-2)

    Plasmid

    #169194

    Purpose

    Baculoviral transfer vector to express SARS-CoV-2 papain-like protease domain (nsp3) in insect cells

    Depositor

    John Diffley

    Article

    Lim et al Biochem J. 2021 Jul 16;478(13):2517-2531

    Insert

    3xFlag-His6-PLpro (ORF1ab SARS-CoV-2, Synthetic)
    

    Use

    Tags

    3xFlag-His6

    Expression

    Insect

    Mutation

    Codon optimised for insect cells (Spodoptera frug…

    Promoter

    Polyhedrin

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGB-GST-3C-mCherry-ATG14/Vps34/Vps15/BECN1

    Plasmid

    #187936

    Purpose

    Plasmid for the expression and purification of GST-3C-mCherry-ATG14/Vps34/Vps15/BECN1 in Spodoptera frugiperda cells (Sf9). Internal reference: SMC1327

    Depositor

    Sascha Martens

    Article

    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

    Inserts

    ATG14 (ATG14 Human)
    VPS34 (PIK3C3 Human)
    VPS15 (PIK3R4 Human)
    BECN1 (BECN1 Human)
    

    Use

    Tags

    GST-mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. plIG-123_HA-GD2-28z_CAR_TF_Library

    Pooled Library

    #207478

    Purpose

    HA-GD2-28z CAR in combination with 100 transcription factors (and related proteins) and 2 controls (GFP/RFP). Library can be used to generate HDR templates for modular pooled knockin (ModPoKI) into th

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Species

    Homo sapiens

    Use

    CRISPR

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

16. plIG-128_HA-GD2-28z_CAR_TFxTF_Library

    Pooled Library

    #207480

    Purpose

    HA-GD2-28z CAR in combination with ~10,000 transcription factor combinations (and related proteins) and controls (GFP/RFP combinations). Library can be used to generate HDR templates for modular poole

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Use

    CRISPR

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

17. plIG-106_NY-ESO-1_TCR_SR_Library

    Pooled Library

    #207474

    Purpose

    NY-ESO-1 TCR in combination with 129 surface receptors and 2 controls (GFP/RFP). Library can be used to generate HDR templates for modular pooled knockin (ModPoKI) into the TRAC locus of human T cells

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Species

    Homo sapiens

    Use

    CRISPR

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

18. plIG-107_NY-ESO-1_TCR_TF_Library

    Pooled Library

    #207475

    Purpose

    NY-ESO-1 TCR in combination with 100 transcription factors (and related proteins) and 2 controls (GFP/RFP). Library can be used to generate HDR templates for modular pooled knockin (ModPoKI) into the

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Species

    Homo sapiens

    Use

    CRISPR

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

19. plIG-109_CD19-BBz_CAR_SR_Library

    Pooled Library

    #207476

    Purpose

    CD19-BBz CAR in combination with 129 surface receptors and 2 controls (GFP/RFP). Library can be used to generate HDR templates for modular pooled knockin (ModPoKI) into the TRAC locus of human T cells

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Use

    CRISPR

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

20. plIG-110_CD19-BBz_CAR_TF_Library

    Pooled Library

    #207477

    Purpose

    CD19-BBz CAR in combination with 100 transcription factors (and related proteins) and 2 controls (GFP/RFP). Library can be used to generate HDR templates for modular pooled knockin (ModPoKI) into the

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Species

    Homo sapiens

    Use

    CRISPR

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only