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hTMEM115-TEV-3xHA-P2A-mNeonGreen HDR template

Plasmid

#214999

Purpose

Endogenously tag human TMEM115 with a C-terminal 3xHA tag in addition to cytosolic mNG for cell sorting.

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

hTMEM115-TEV-3xHA-P2A-mNeonGreen HDR template (TMEM115 Human)

Use

Hdr template

Tags

3xHA

Expression

Mutation

Promoter

None

Available since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pUC57-2xFLAG-mAID-AMBRA1 HDR donor template

Plasmid

#172609

Purpose

Donor plasmid containing the HDR template for the in-frame, N-terminal 2xFLAG-mAID tagging of AMBRA1 gene

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

2xFLAG-mAID-AMBRA1 (AMBRA1 Human, Synthetic)

Use

CRISPR; Human targeting

Tags

Expression

Mutation

Promoter

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCRII-TOPO CMV-cGFP-mMALAT1_3' Comp.15 Sense

Plasmid

#46839

Purpose

Express cGFP transcript ending in a mutant MALAT1 triple helix that causes the transcript to be stable but poorly translated

Depositor

Phil Sharp

Article

Wilusz et al Genes Dev. 2012 Nov 1;26(21):2392-407. d

Insert

Coral Green Fluorescent Protein (cGFP)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 25, 2013

Availability

Academic Institutions and Nonprofits only

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pCRII-TOPO CMV-cGFP-mMALAT1_3' Comp.14 Sense

Plasmid

#46838

Purpose

Express cGFP transcript ending in the minimal functional MALAT1 triple helix

Depositor

Phil Sharp

Article

Wilusz et al Genes Dev. 2012 Nov 1;26(21):2392-407. d

Insert

Coral Green Fluorescent Protein (cGFP)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 25, 2013

Availability

Academic Institutions and Nonprofits only

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pCRII-TOPO CMV-cGFP-mMALAT1_3' Mut U1/U2 Sense

Plasmid

#46837

Purpose

Express cGFP transcript ending in a mutant MALAT1 triple helix which causes the transcript to be degraded

Depositor

Phil Sharp

Article

Wilusz et al Genes Dev. 2012 Nov 1;26(21):2392-407. d

Insert

Coral Green Fluorescent Protein (cGFP)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 25, 2013

Availability

Academic Institutions and Nonprofits only

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Enterovirus A71 (EV-A71) isolate TW-2553-1998 3C protease

Plasmid

#228636

Purpose

Bacterial expression of the A71 3C protease (isoform B) used in crystallisation

Depositor

ASAP Discovery, Frank von Delft

Insert

Enterovirus A71 3C protease isoform B

Use

Tags

His-SUMO

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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T7-LacO-Halo-RGD-PILATeS (200nM, Force Inert)

Plasmid

#216717

Purpose

Expresses HaloTag-RGD-PILATeS Force Inert Control with 200 nM BiT tag in bacteria

Depositor

Alex Dunn

Article

Zhong et al ACS Sens. 2024 Jul 26;9(7):3489-3495. do

Insert

HaloTag-RGD-PILATeS

Use

Tags

6xHis and HaloTag

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

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T7-LacO-Halo-RGD-PILATeS (700pM, Force Inert)

Plasmid

#216715

Purpose

Expresses HaloTag-RGD-PILATeS Force Inert Control with 700 pM BiT tag in bacteria

Depositor

Alex Dunn

Article

Zhong et al ACS Sens. 2024 Jul 26;9(7):3489-3495. do

Insert

HaloTag-RGD-PILATeS

Use

Tags

6xHis and HaloTag

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-CrPV-Rluc-6xbsMS2-PolyA-Malat_AC

Plasmid

#148446

Purpose

Mammalian Expression of 6xbsMS2-PolyA-Malat

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

6xbsMS2-PolyA-Malat

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HCV-RLuc-6xbsMS2-polyA-Malat_AC

Plasmid

#148448

Purpose

Mammalian Expression of 6xbsMS2-polyA-Malat

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

6xbsMS2-polyA-Malat

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pET-15b(+) Toxoplasma gondii (delta prenylation motif)

Plasmid

#170789

Purpose

Expresses pET-15b(+) Toxoplasma gondii (delta prenylation motif)

Depositor

Kalle Gehring

Insert

Toxoplasma gondii PRL

Use

Tags

N-terminal His-tag

Expression

Bacterial

Mutation

Deleted nucleotides coding for 4 C-terminal amino…

Promoter

AmpR, lacI

Available since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pET-15b(+) Plasmodium falciparum PRL (Δprenylation motif)

Plasmid

#166422

Purpose

Express pET-15b(+) Plasmodium falciparum PRL (Δprenylation motif)

Depositor

Kalle Gehring

Insert

Plasmodium falciparum PRL

Use

Tags

N-terminal His-tag

Expression

Bacterial

Mutation

Deleted nucleotides coding for 4 C-terminal amino…

Promoter

AmpR, lac1

Available since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pET-15b(+) Pythium oligandrum PRL (Δprenylation motif)

Plasmid

#166423

Purpose

Express pET-15b(+) Pythium oligandrum PRL (Δprenylation motif)

Depositor

Kalle Gehring

Article

Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

Insert

Pythium oligandrum PRL

Use

Tags

N-terminal His-tag

Expression

Bacterial

Mutation

Deleted nucleotides coding for 4 C-terminal amino…

Promoter

AmpR, lac1

Available since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pET-15b(+) Reticulomyxa filosa PRL (Δprenylation motif)

Plasmid

#166424

Purpose

Express pET-15b(+) Reticulomyxa filosa PRL (Δprenylation motif)

Depositor

Kalle Gehring

Article

Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

Insert

Reticulomyxa filosa PRL

Use

Tags

N-terminal His-tag

Expression

Bacterial

Mutation

Deleted nucleotides coding for 4 C-terminal amino…

Promoter

AmpR, lac1

Available since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pET-15b(+) Sphaeforma arctica PRL (Δprenylation motif)

Plasmid

#166425

Purpose

Express pET-15b(+) Sphaeforma arctica PRL (Δprenylation motif)

Depositor

Kalle Gehring

Article

Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

Insert

Sphaeforma arctica PRL

Use

Tags

N-terminal His-tag

Expression

Bacterial

Mutation

Deleted nucleotides coding for 4 C-terminal amino…

Promoter

AmpR, lac1

Available since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pET-15b(+) Acytostelium subglobosum PRL (Δprenylation motif)

Plasmid

#166415

Purpose

Express pET-15b(+) Acytostelium subglobosum PRL (Δprenylation motif)

Depositor

Kalle Gehring

Article

Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

Insert

Acytostelium subglobosum PRL

Use

Tags

N-terminal His-tag

Expression

Bacterial

Mutation

Deleted nucleotides coding for 4 C-terminal amino…

Promoter

AmpR, lac1

Available since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pET-15b(+) Caenorhabditis elegans PRL (Δprenylation motif)

Plasmid

#166416

Purpose

Express pET-15b(+) Caenorhabditis elegans PRL (Δprenylation motif)

Depositor

Kalle Gehring

Article

Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

Insert

caenorhabditis elegans PRL

Use

Tags

N-terminal His-tag

Expression

Bacterial

Mutation

Deleted nucleotides coding for 4 C-terminal amino…

Promoter

AmpR, lac1

Available since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pET-15b(+) Danio rerio PRL (Δprenylation motif)

Plasmid

#166417

Purpose

Express pET-15b(+) Danio rerio PRL (Δprenylation motif)

Depositor

Kalle Gehring

Article

Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

Insert

Danio rerio PRL (ptp4a3a Zebrafish)

Use

Tags

N-terminal His-tag

Expression

Bacterial

Mutation

Deleted nucleotides coding for 4 C-terminal amino…

Promoter

AmpR, lac1

Available since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pET-15b(+) Drosophila melanogaster PRL (Δprenylation motif)

Plasmid

#166418

Purpose

Express pET-15b(+) Drosophila melanogaster PRL (Δprenylation motif)

Depositor

Kalle Gehring

Article

Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

Insert

Drosophila melanogaster PRL (Prl Fly)

Use

Tags

N-terminal His-tag

Expression

Bacterial

Mutation

Deleted nucleotides coding for 4 C-terminal amino…

Promoter

AmpR, lac1

Available since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pET-15b(+) Galdieria sulphuraria PRL (Δprenylation motif)

Plasmid

#166419

Purpose

Express pET-15b(+) Galdieria sulphuraria PRL (Δprenylation motif)

Depositor

Kalle Gehring

Article

Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

Insert

Galdieria sulphuraria PRL

Use

Tags

N-terminal His-tag

Expression

Bacterial

Mutation

Deleted nucleotides coding for 4 C-terminal amino…

Promoter

AmpR, lac1

Available since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 281 - 300 of 38473 results

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