Addgene

We narrowed to 357 results for: NS1

Showing: 281 - 300 of 357 results

Results per page:

Sort results by:

pGEX4T3-HA-ERK2 D321N

Plasmid

#53202

Purpose

Expresses GST and HA tagged human ERK2 D321N from bacterial expression vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

ERK2 (MAPK1 Human)

Use

Tags

GST and HA

Expression

Bacterial

Mutation

D321N

Promoter

Available since

June 24, 2014

Availability

Academic Institutions and Nonprofits only
pB80-KIF1A(1-383)-GFP-SSPB(milli)

Plasmid

#174626

Purpose

Constitutively active KIF1A for optogenetic heterodimerization to iLID via SSPB(milli)

Depositor

Lukas Kapitein

Article

Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

KIF1A(1-383)-GFP-SSPB(milli) (Kif1a Mouse, Synthetic)

Use

Tags

GFP-SSPB(milli)

Expression

Mammalian

Mutation

mmKIF1A(aa1-383): Pro202Ala; EGFP: Met1Del; SSPB:…

Promoter

Chicken beta-actin

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA4-His-Max-A-PQBP1

Plasmid

#25802

Depositor

Marius Sudol

Article

Tapia et al J Biol Chem. 2010 Apr 21. ():.

Insert

PQBP1 (PQBP1 Human)

Use

Tags

His and Xpress

Expression

Mammalian

Mutation

Promoter

Available since

July 21, 2010

Availability

Academic Institutions and Nonprofits only
pB80-KIF1A(1-383)-GFP-SSPB(nano)

Plasmid

#174628

Purpose

Constitutively active KIF1A for optogenetic heterodimerization to iLID via SSPB(nano)

Depositor

Lukas Kapitein

Article

Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

KIF1A(1-383)-GFP-SSPB(nano) (Kif1a Mouse, Synthetic)

Use

Tags

GFP-SSPB(nano)

Expression

Mammalian

Mutation

mmKIF1A(aa1-383): Pro202Ala; EGFP: Met1Del;

Promoter

Chicken beta-actin

Available since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pB80-KIF1A(1-365)-GFP-SSPB(micro)

Plasmid

#174629

Purpose

Non-processive monomeric KIF1A for optogenetic heterodimerization to iLID via SSPB(micro)

Depositor

Lukas Kapitein

Article

Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

KIF1A(1-365)-GFP-SSPB(micro) (Kif1a Mouse, Synthetic)

Use

Tags

GFP-SSPB(micro)

Expression

Mammalian

Mutation

mmKIF1A(aa1-365): Pro202Ala; EGFP: Met1Del; SSPB:…

Promoter

Chicken beta-actin

Available since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
LCV2 PQBP1 KO2

Plasmid

#217448

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting human PQBP1

Depositor

Jarrod Johnson

Article

Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

Insert

sgRNA targeting PQBP1 (PQBP1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
LCV2 PQBP1 KO1

Plasmid

#217447

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting human PQBP1

Depositor

Jarrod Johnson

Article

Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

Insert

sgRNA targeting PQBP1 (PQBP1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pD649-HAsp-SIRPD-COMP5AP-AviTag-9xHis

Plasmid

#157128

Purpose

Mammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

SIRPD (SIRPD Human)

Use

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Mutation

Promoter

CMV/SP6

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pB80-hsKIF5B(1-353)GCN4-L-GFP

Plasmid

#193714

Purpose

Expression of dimeric, GFP tagged, human kinesin-1 (KIF5B aa1-353)

Depositor

Lukas Kapitein

Article

Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

Insert

hsKIF5B(1-353)-GCN4-L-GFP (KIF5B Synthetic, Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

hsKIF5B(aa1-353);

Promoter

Chicken beta-actin

Available since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pB80-hsKIF5B(1-353)-GCN4-L-mCherry

Plasmid

#193715

Purpose

Expression of dimeric, mCherry tagged, human kinesin-1 (KIF5B aa1-353)

Depositor

Lukas Kapitein

Article

Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

Insert

hsKIF5B(1-353)-GCN4-L-mCherry (KIF5B Synthetic, Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

hsKIF5B(aa1-353); mCherry: Met1 Deleted

Promoter

Chicken beta-actin

Available since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pB80-KIF1A(1-365)-mVenus-SSPB(micro)

Plasmid

#174635

Purpose

Non-processive monomeric KIF1A for optogenetic heterodimerization to iLID via SSPB(micro)

Depositor

Lukas Kapitein

Article

Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

KIF1A(1-365)-mVenus-SSPB(micro) (Kif1a Mouse, Synthetic)

Use

Tags

mVenus-SSPB(micro)

Expression

Mammalian

Mutation

mmKIF1A(aa1-365): Pro202Ala; mVenus: Met1Del, Thr…

Promoter

Chicken beta-actin

Available since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pB80-KIF1A(1-383)-GFP-SSPB(micro)

Plasmid

#174627

Purpose

Constitutively active KIF1A for optogenetic heterodimerization to iLID via SSPB(micro)

Depositor

Lukas Kapitein

Article

Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

KIF1A(1-383)-GFP-SSPB(micro) (Kif1a Mouse, Synthetic)

Use

Tags

GFP-SSPB(micro)

Expression

Mammalian

Mutation

mmKIF1A(aa1-383): Pro202Ala; EGFP: Met1Del; SSPB:…

Promoter

Chicken beta-actin

Available since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pB80-KIF1A(1-365)-VVDfast-mVenus-SSPB(micro)

Plasmid

#174637

Purpose

Opto-kinesin for optogenetic heterodimerization to iLID via SSPB(micro)

Depositor

Lukas Kapitein

Article

Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

KIF1A(1-365)-VVDfast-mVenus-SSPB(micro) (Kif1a Mouse, Synthetic)

Use

Tags

mVenus-SSPB(micro)

Expression

Mammalian

Mutation

mmKIF1A(aa1-365): Pro202Ala; ncVVD(aa37-184): Ile…

Promoter

Chicken beta-actin

Available since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pB80-KIF1A(1-365)-VVD52C-mVenus-SSPB(micro)

Plasmid

#174636

Purpose

Poorly reversible opto-kinesin for optogenetic heterodimerization to iLID via SSPB(micro)

Depositor

Lukas Kapitein

Article

Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

KIF1A(1-365)-VVD52C-mVenus-SSPB(micro) (Kif1a Mouse, Synthetic)

Use

Tags

mVenus-SSPB(micro)

Expression

Mammalian

Mutation

mmKIF1A(aa1-365): Pro202Ala; ncVVD(aa37-184): Ile…

Promoter

Chicken beta-actin

Available since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pHTN_HaloTag_KIF5B

Plasmid

#213404

Purpose

Full-length human kinesin-1 with HaloTag at the N-terminus

Depositor

Jonas Ries

Article

Deguchi et al Science. 2023 Mar 10;379(6636):1010-1015

Insert

HaloTag-KIF5B (KIF5B Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHTN_HaloTag_KIF5B[1-560]

Plasmid

#213403

Purpose

Truncated human kinesin-1 with HaloTag at the N-terminus

Depositor

Jonas Ries

Article

Deguchi et al Science. 2023 Mar 10;379(6636):1010-1015

Insert

HaloTag-KIF5B[1-560] (KIF5B Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE-PTCH1

Plasmid

#116779

Purpose

Lentiviral expression of PTCH1

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PTCH1 (PTCH1 Human)

Use

Lentiviral

Tags

Expression

Mutation

WT

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pB80-KIF1A(1-365)-VVD52C-GFP-SSPB(micro)

Plasmid

#174630

Purpose

Poorly reversible opto-kinesin for optogenetic heterodimerization to iLID via SSPB(micro)

Depositor

Lukas Kapitein

Article

Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

KIF1A(1-365)-VVD52C-GFP-SSPB(micro) (Kif1a Mouse, Synthetic)

Use

Tags

GFP-SSPB(micro)

Expression

Mammalian

Mutation

mmKIF1A(aa1-365): Pro202Ala; ncVVD(aa37-184): Ile…

Promoter

Chicken beta-actin

Available since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pB80-KIF1A(1-365)-VVDfast-GFP-SSPB(micro)

Plasmid

#174631

Purpose

Opto-kinesin for optogenetic heterodimerization to iLID via SSPB(micro)

Depositor

Lukas Kapitein

Article

Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

KIF1A(1-365)-VVDfast-GFP-SSPB(micro) (Kif1a Mouse, Synthetic)

Use

Tags

GFP-SSPB(micro)

Expression

Mammalian

Mutation

mmKIF1A(aa1-365): Pro202Ala; ncVVD(aa37-184): Ile…

Promoter

Chicken beta-actin

Available since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pB80-KIF1A(1-365)-VVD52C-mCherry-SSPB(micro)

Plasmid

#174633

Purpose

Poorly reversible opto-kinesin for optogenetic heterodimerization to iLID via SSPB(micro)

Depositor

Lukas Kapitein

Article

Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

KIF1A(1-365)-VVD52C-mCherry-SSPB(micro) (Kif1a Mouse, Synthetic)

Use

Tags

mCherry-SSPB(micro)

Expression

Mammalian

Mutation

mmKIF1A(aa1-365): Pro202Ala; ncVVD(aa37-184): Ile…

Promoter

Chicken beta-actin

Available since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge