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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 3,869 results for: SPL;

Showing: 281 - 300 of 3869 results

  1. hSyn-pDisplay-SNAP[C145A]-T2A-EGFP

    Plasmid
    #223504
    Purpose
    Co-expression of dead-mutant SNAP-tags to outer surface in mammalian cells coupled with an EGFP transfection marker
    Depositor
    James Frank
    Insert
    dead-mutant SNAP-tag and EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    SNAP-tag C145A
    Promoter
    hSyn
    Available since
    Aug. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    223504-plasmid-map-sequence-id-443304

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  2. HA Display H3_A/Hong Kong/1/1968 Part 4-DO

    Plasmid
    #206518
    Purpose
    CcdB Dropout vector for part 4 of Hemagglutinin surface expression in mammalian cells
    Depositor
    IIya Finkelstein
    Type
    Empty backbone
    Use
    Tags
    3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    206518-plasmid-map-sequence-id-408109

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  3. HA Display H3_A/Hong Kong/1/1968 Part 1-DO

    Plasmid
    #206515
    Purpose
    CcdB Dropout vector for part 1 of Hemagglutinin surface expression in mammalian cells
    Depositor
    IIya Finkelstein
    Type
    Empty backbone
    Use
    Tags
    3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    206515-plasmid-map-sequence-id-408101

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  18. N-Terminal Split Cas9 D10A Nickase with GyrA intein

    Plasmid
    #58695
    Purpose
    Expresses N-terminus of D10A SpCas9 nickase domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with C-Terminal Split Cas9 Gyra Intein for full length SpCas9 nickase production
    Depositor
    Gang Bao
    Article
    Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103
    Insert
    D10A Nickase humanized S. pyogenes Cas9 with Gyra Nsplit Intein
    Use
    AAV and CRISPR
    Tags
    GyrA Nsplit Intein, HA Tag, and NLS
    Expression
    Mammalian
    Mutation
    D10A nickase converting mutation to SpCas9
    Promoter
    CBh
    Available since
    Sept. 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    58695-plasmid-map-sequence-id-90694

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  20. pNAS1b split mCherry NEDD4 WW2 pos ctrl

    Plasmid
    #112029
    Purpose
    Mode #2 phosphosite positive control (split mCherry, using NEDD4 WW2 binding domain paired with proline-rich positive control peptide)
    Depositor
    Jesse Rinehart
    Article
    Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.
    Insert
    NEDD4 WW2 domain fused to C-terminal split mCherry, proline-rich pos ctrl interacting peptide fused to N-terminal split mCherry
    Use
    Tags
    Split mCherry
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    112029-plasmid-map-sequence-id-213508

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Showing: 281 - 300 of 3869 results