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We narrowed to 1,112 results for: cas12a

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Showing: 281 - 300 of 1112 results

  3. pUPD2 AtU6-26:LbCas12a_DR (GB1443)

    Plasmid
    #160575
    Purpose
    LbCas12a direct repeat sequence fused to the A. thaliana U6-26 promoter used for the assembly of gRNA expression cassettes.
    Depositor
    Diego Orzaez
    Publication
    Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AtU6-26:LbCas12a_DR
    Use
    CRISPR and Synthetic Biology
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    U6-26
    Available since
    Jan. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    160575-plasmid-map-sequence-id-322087

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  4. pCMV-dCas12a-ABE8e-V106W

    Plasmid
    #193646
    Purpose
    Expression of base editor dCas12a-ABE8e-V106W in mammalian cells for base editing, along with non-fused EGFP fluorescence
    Depositor
    Liangxue Lai
    Publication
    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dLbCas12a-TadA*8e-V106W, EGFP
    Expression
    Mammalian
    Mutation
    TadA*8e-V106W
    Promoter
    CMV
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    193646-plasmid-map-sequence-id-422057

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  5. pCMV-enCas12a-ABE8e-V106W

    Plasmid
    #193653
    Purpose
    Expression of base editor enCas12a-ABE8e-V106W in mammalian cells for base editing, along with non-fused EGFP fluorescence
    Depositor
    Liangxue Lai
    Publication
    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dLb-enCas12a-TadA*8e-V106W, EGFP
    Expression
    Mammalian
    Mutation
    dLbCas12a-D156R/G532R/K538R, TadA*8e-V106W
    Promoter
    CMV
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    193653-plasmid-map-sequence-id-422063

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  6. pY036_ATP1A1_G3

    Plasmid
    #86617
    Purpose
    Expresses the ATP1A1 G3 crRNA in combination with AsCpf1-3xHA to target ATP1A1 exon 4. U6-crRNA(ATP1A1 G3)-CBh-AsCpf1
    Depositor
    Yannick Doyon
    Publication
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Insert
    ATP1A1 G3 crRNA + AsCpf1-3xHA
    Use
    CRISPR; Co-selection via nhej using ouabain
    Tags
    3xHA
    Expression
    Mammalian
    Promoter
    CBh
    Available since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    86617-plasmid-map-sequence-id-168852

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  8. pCMV-dCas12a-ABE7

    Plasmid
    #193644
    Purpose
    Expression of base editor dCas12a-ABE7 in mammalian cells for base editing, along with non-fused EGFP fluorescence
    Depositor
    Liangxue Lai
    Publication
    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dLbCas12a-TadA-TadA*7.10, EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    193644-plasmid-map-sequence-id-421946

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  9. pCAG-LbCas12a-Plus-mCherry

    Plasmid
    #191230
    Purpose
    To express the LbCas12a nuclease variant "LbCas12a-Plus" that enhanced the editing activity and specificity
    Depositor
    Zhili Rong
    Publication
    Huang et al BMC Biol. 2022 Apr 25;20(1):91. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LbCas12a-Plus-P2A-mCherry
    Use
    CRISPR
    Mutation
    D156R, R883K, R887A
    Available since
    Aug. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    191230-plasmid-map-sequence-id-404705

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  11. pET-28b-T7-hAsCas12a-NLS(nucleoplasmin)-6xHis (BPK3541)

    Plasmid
    #114069
    Purpose
    T7 promoter bacterial expression plasmid for human codon optimized AsCas12a with C-terminal NLS and His-tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized AsCas12a with C-terminal NLS and His-tag
    Tags
    NLS(nucleoplasmin) and 6xHis
    Expression
    Bacterial
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    114069-plasmid-map-sequence-id-220520

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  14. pUC19-U6-FnCas12a crRNA-BsmBI cassette (BPK4446)

    Plasmid
    #114087
    Purpose
    U6 promoter crRNA entry vector used for all FnCas12a crRNAs (clone spacer oligos into BsmBI cassette)
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    entry vector for FnCas12a crRNAs
    Expression
    Mammalian
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    114087-plasmid-map-sequence-id-220506

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  15. pMB22.SV40.Zeo

    Plasmid
    #135692
    Purpose
    Lentivirus delivery for stable expression of AsCas12a-VPR, Zeocin selection
    Depositor
    Florian Karreth
    Insert
    mammalian codon-optimized Acidaminococcus sp. Cas12a fused to VPR activator
    Use
    Lentiviral
    Available since
    Feb. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    135692-plasmid-map-sequence-id-322223

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  18. pCAG-hFnCas12a-NLS(nucleoplasmin)-3xHA (AAS1472)

    Plasmid
    #114089
    Purpose
    CAG promoter expression plasmid for human codon optimized FnCas12a nuclease with C-terminal NLS and HA tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized FnCas12a
    Tags
    NLS(nucleoplasmin) and 3xHA
    Expression
    Mammalian
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    114089-plasmid-map-sequence-id-220552

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  19. pCMV-dCas12a-A3A-Y132D

    Plasmid
    #193641
    Purpose
    Expression of base editor dCas12a-A3A-Y132D in mammalian cells for base editing
    Depositor
    Liangxue Lai
    Publication
    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dLbCas12a-hA3A-Y132D
    Expression
    Mammalian
    Mutation
    hAPOBEC3A-Y132D
    Promoter
    CMV
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    193641-plasmid-map-sequence-id-421939

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  20. Cas12aUltra_5XNLS

    Plasmid
    #218775
    Purpose
    His-TEV-Cas12aUltra-BPSV40_cMyc_NP_5NLS
    Depositor
    Ross Wilson
    Publication
    Sahu et al Nat Protoc. 2025 Mar 3. doi: 10.1038/s41
    Insert
    5xNLS-BPSV40-NLP-cMyc-cMyc-BPSV40- AspCas12a
    Use
    CRISPR
    Tags
    6XHis (C terminal), HA, Nucleoplasmin, SV40, and …
    Expression
    Bacterial
    Mutation
    Nucleotides mutations were made to encode for arg…
    Available since
    Aug. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    218775-plasmid-map-sequence-id-443448

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Showing: 281 - 300 of 1112 results