We narrowed to 2,567 results for: gem

1. pGEM cLrdr (CT#900)

   Plasmid

   #13950

   Depositor

   Cliff Tabin

   Article

   Essner et al Nature. 2002 Jul 4. 418(6893):37-8.

   Insert

   Lrdr
   

   Expression

   Bacterial

   Available Since

   Feb. 16, 2007

   Availability

   Academic Institutions and Nonprofits only

2. pCSII-EF-miRFP670v1-hGem(1/110)

   Plasmid

   #80006

   Purpose

   hGem(1/110) fused with near-infrared fluorescent protein miRFP670v1 (S/G2/M phase cell cycle reporter)

   Depositor

   Vladislav Verkhusha

   Article

   Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

   Insert

   hGeminin (1/110) (GMNN Human)
   

   Use

   Lentiviral

   Tags

   miRFP670v1

   Promoter

   EF-1 alpha

   Available Since

   July 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEMS-II-dCas13-APO1-YTH

   Plasmid

   #204767

   Purpose

   GEMS system using dCas13-tethered APO1-YTH (pCMV-EGFP-PEST-m6A sensor-DHFR; EF1a-dCas13-NLS-APO1-YTH; hPGK-DsRed)

   Depositor

   Kate Meyer

   Article

   Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

   Insert

   dCas13-NLS-APO1-YTH-HA
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   Ef1alpha

   Available Since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEMHE-mScarlet-MAP4-MTBD

   Plasmid

   #171488

   Purpose

   T7 promotor drives in vitro transcription of mScarlet-tagged mouse MAP4-Microtubule Binding Domain mRNA

   Depositor

   Melina Schuh

   Article

   Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

   Insert

   MAP4-MTBD (Map4 Mouse)
   

   Use

   Pgehme

   Tags

   mScarlet

   Mutation

   Aminoacids 658-1126

   Promoter

   T7

   Available Since

   July 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEMHE-miRFP-MAP4-MTBD

   Plasmid

   #171487

   Purpose

   T7 promotor drives in vitro transcription of miRFP-tagged mouse MAP4-Microtubule Binding Domain mRNA

   Depositor

   Melina Schuh

   Article

   Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

   Insert

   MAP4-MTBD (Map4 Mouse)
   

   Use

   Pgehme

   Tags

   miRFP

   Mutation

   Aminoacids 658-1126

   Promoter

   T7

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. YL-CashGem-BAT1-URA3-LINEAR

   Plasmid

   #174837

   Purpose

   pRS414 backbone containing LINEAR fragment targeting BAT1 in Y. lipolytica

   Depositor

   Zengyi Shao

   Article

   Ploessl et al Nat Chem Biol. 2021 Oct 28. pii: 10.1038

   Insert

   Codon optimized Cas9:hGem
   

   Use

   CRISPR and Synthetic Biology

   Tags

   hGeminin tag

   Expression

   Bacterial and Yeast

   Promoter

   GPDp

   Available Since

   Jan. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. CMV-mito-GEM-GECO1

   Plasmid

   #32461

   Purpose

   Mammalian expression of mitochondria-targeting blue-green emission ratiometric genetically encoded Ca2+ indicator for optical imaging

   Depositor

   Robert Campbell

   Article

   Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

   Insert

   GEM-GECO
   

   Tags

   a duplex of the mitochondrial targeting signal of…

   Expression

   Mammalian

   Mutation

   GCaMP3 L60P/K69E/N77Y/D86G/N98I/K119I/L173Q/T223S…

   Promoter

   CMV

   Available Since

   Dec. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEM-GFP-URA3-GFP

   Plasmid

   #72606

   Purpose

   Template for creating a C-terminal GFP tag AND an internal GFP tag from a single transfection of yeast

   Depositor

   Bruce Granger

   Article

   Granger et al PLoS One. 2018 Jan 12;13(1):e0191194. do

   Inserts

   GFP (full)
   URA3
   GFP (partial)
   Optional Linker
   

   Mutation

   Includes full 819 bp coding sequence of URA3, 435…

   Available Since

   Jan. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGem-PgpdA-Cre-TtrpC

   Plasmid

   #168784

   Purpose

   Helper vector for the transient expression of Cre recombinase in filamentous fungi. Not replicative in fungi.

   Depositor

   Yit Heng Chooi

   Article

   Roux et al ACS Synth Biol. 2022 Feb 16. doi: 10.102

   Insert

   Cre
   

   Use

   Cre/Lox and Synthetic Biology; Expression in fila…

   Promoter

   PgpdA

   Available Since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEMT-Mef2c-tPT2A-GFP

    Plasmid

    #111771

    Purpose

    Bicistronic construct: Co-express two genes by 2A peptides

    Depositor

    Li Qian

    Article

    Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

    Insert

    Mef2c-tPT2A-GFP (Mef2c Mouse)
    

    Use

    Cloning intermediate

    Tags

    GFP

    Promoter

    T7

    Available Since

    Aug. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. gRNA3_NIPBL_Cas9-hGem-for_N-terminal_tagging

    Plasmid

    #217662

    Purpose

    expresses Cas9-hGem and guideRNA for N terminal tagging of NIPBL

    Depositor

    Daniel Gerlich

    Article

    Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

    Insert

    sgRNA3 for N terminal tagging of NIPBL (NIPBL Human)
    

    Use

    CRISPR

    Expression

    Bacterial and Mammalian

    Promoter

    U6

    Available Since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCashGem-Xyl2-URA3-LINEAR

    Plasmid

    #174840

    Purpose

    pRS414 backbone containing LINEAR fragment targeting XYL2 in S. stipitis

    Depositor

    Zengyi Shao

    Article

    Ploessl et al Nat Chem Biol. 2021 Oct 28. pii: 10.1038

    Insert

    Codon optimized Cas9:hGem
    

    Use

    CRISPR and Synthetic Biology

    Tags

    hGeminin tag

    Expression

    Bacterial and Yeast

    Promoter

    ENO1p

    Available Since

    Jan. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAVS1_Puro_hPGK1_hGEM(1-110)_mNG_hSLBP(18-108)

    Plasmid

    #176932

    Purpose

    Donor for targeted integration of S-phase specific probe (FUCCI-S) to the AAVS1 safe harbor locus

    Depositor

    Yannick Doyon

    Insert

    hGEM(1-110)_mNG_hSLBP(18-108)
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    PGK1

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEM-sgMUC1_dual-MTS-3x-Set I

    Plasmid

    #162763

    Purpose

    Separately expressing 3 unique sgMUC1_dual-MTS (SetI)

    Depositor

    Antony K Chen

    Article

    Mao et al Nucleic Acids Res. 2019 Nov 18;47(20):e1

    Insert

    sgMUC1_dual-MTS_1; sgMUC1_dual-MTS_2; sgMUC1_dual-MTS_3
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEMHE-mClover3-MAP4-MTBD

    Plasmid

    #171498

    Purpose

    T7 promotor drives in vitro transcription of mClover3-tagged mouse MAP4-Microtubule Binding Domain mRNA

    Depositor

    Melina Schuh

    Article

    So et al Science. 2019 Jun 28;364(6447). pii: 364

    Insert

    MAP4-MTBD (Map4 Mouse)
    

    Use

    Pgehme

    Tags

    mClover3

    Promoter

    T7

    Available Since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEM-3XP3.RFP-tan

    Plasmid

    #83474

    Purpose

    RFP selectable marker for use in two-stage CRISPR-mediated allele replacement strategies in Drosophila. D. americana tan homology arms can be replaced with locus of choice.

    Depositor

    Patricia Wittkopp

    Article

    Lamb et al Fly (Austin). 2016 Aug 5:1-12.

    Insert

    3XP3.RFP-tan
    

    Use

    CRISPR

    Expression

    Bacterial and Insect

    Promoter

    3XP3 eye-expression Promoter

    Available Since

    Oct. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEMHE-mCherry-mTrim21ΔRING-Box

    Plasmid

    #105523

    Purpose

    T7 promotor drives in vitro transcription of mCherry-tagged mouse TRIM21 with deleted RING-box mRNA

    Depositor

    Melina Schuh

    Article

    Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

    Insert

    TRIM21 (Trim21 Mouse)
    

    Use

    In vitro transcription

    Tags

    mCherry

    Mutation

    deletion of RING domain (aa124-462)

    Promoter

    T7

    Available Since

    March 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1+ GEMIN6 myc HIS

    Plasmid

    #71659

    Purpose

    Mammalian expression of GEMIN6

    Depositor

    Gideon Dreyfuss

    Insert

    GEMIN6 (GEMIN6 Human)
    

    Tags

    HIS and myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

19. Rab27a cds in pGem-T Easy

    Plasmid

    #80546

    Purpose

    Can be used for in situ hybridisation or other purposes

    Depositor

    Rob Parton

    Article

    Hall et al Cell Logist. 2017 Mar 1;7(1):e1301151. d

    Insert

    rab27a (rab27a Zebrafish)
    

    Available Since

    April 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEM mouse PEA3 [TJ#200]

    Plasmid

    #16346

    Depositor

    Thomas Jessell

    Insert

    PEA3 (Etv4 Mouse)
    

    Expression

    Bacterial

    Available Since

    Feb. 4, 2009

    Availability

    Academic Institutions and Nonprofits only