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pEF1-Pls1-sfGFP

Plasmid

#205748

Purpose

Expresses sfGFP under IPTG inducible promoter Pls1 in high copy pTRKH2 gram-positive shuttle vector. Used in Lactobacillus gasseri. Low strength.

Depositor

Jerome Bonnet

Publication

Fristot et al ACS Synth Biol. 2024 Mar 15;13(3):951-95

Type

Empty backbone

Use

Shuttle vector gram+ gram-

Expression

Bacterial

Promoter

Pls1 (Phyperspank mutant, IPTG inducible)

Available since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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Tubulin-mhYFP

Plasmid

#186525

Purpose

Localization of monomeric hyperfolder YFP fluorescent protein to tubulin

Depositor

Gregory Petsko

Publication

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

Monomeric hyperfolder YFP

Tags

Tubulin

Expression

Mammalian

Mutation

hfYFP-S147P/L195M/V206K

Promoter

CMV

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pFX-mCherry-KRasCT

Plasmid

#175835

Purpose

Express mCherry with plasma membrane-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Publication

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

mCherry with plasma membrane-targeting sequence

Tags

C-terminal hypervariable region of K-Ras 4B

Expression

Mammalian

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pXJ-Myc-IRF4

Plasmid

#232667

Purpose

transient expresses IRF4 in mammalian cells

Depositor

Chen-Yang Shen

Publication

Chen et al HGG Adv. 2026 May 25:100630. doi: 10.101

Insert

IRF4 (IRF4 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available since

March 7, 2025

Availability

Academic Institutions and Nonprofits only

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pLKO.1P POLE1_1

Plasmid

#160762

Purpose

Suppress POLE1

Depositor

Richard Possemato

Publication

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shPOLE1_1

Use

Lentiviral

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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scADGFP

Plasmid

#114434

Purpose

Epilepsy promoter enhanced with Arc elements driving GFP with SV40E polyA

Depositor

Edward Perez-Reyes

Publication

Burke et al Int J Mol Sci. 2023 Sep 23;24(19):14467.

Insert

Green fluorescent protein

Use

AAV

Tags

ER export sequence FCYENEV

Expression

Mammalian

Promoter

Novel synthetic activity-dependent promoter

Available since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pUBC_IGF2BP2(IMP2)_serinemod_E488QADAR_p2A_yGFP

Plasmid

#247301

Purpose

Expresses Igf2bp2 (IMP2) fused to hyperactive ADAR catalytic domain, mammalian expression vector for HyperTRIBE

Depositor

Robert Singer

Publication

Do et al NAR Mol Med, 2026

Insert

Adenosine deaminse acting on RNA (Adar Synthetic, Fly)

Tags

GFP and V5

Expression

Mammalian

Mutation

E488Q, hyperactive variant

Promoter

UBC

Available since

June 29, 2026

Availability

Academic Institutions and Nonprofits only

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pRL021

Plasmid

#250166

Purpose

DGRec 2-plasmid system: encodes CspRecT, MutL* and a dgrRNA cloning site (containing ccdB toxin, to replace with TR).

Depositor

David Bikard

Publication

Rochette et al Nat Biotechnol. 2026 Apr 16. doi: 10.103

Type

Empty backbone

Expression

Bacterial

Available since

Aug. 11, 2026

Availability

Academic Institutions and Nonprofits only

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pAcBac1-BacMam-4x-PyOtR

Plasmid

#217364

Purpose

Expresses four copies pyrrolysyl tRNA variant "PyOtR" with U6 promoter; can be packaged in bacmid

Depositor

Abhishek Chatterjee

Publication

Jewel et al Angew Chem Int Ed Engl. 2024 Jan 26:e202

Insert

4 copies of pyrrolysyl-tRNA mutant PyOtR

Use

Baculoviral

Expression

Insect and Mammalian

Promoter

U6

Available since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pLAS2w.Phyg-HAVCR1

Plasmid

#232669

Purpose

expresses HAVCR1 in mammalian cells

Depositor

Chen-Yang Shen

Publication

Chen et al HGG Adv. 2026 May 25:100630. doi: 10.101

Insert

HAVCR1 (HAVCR1 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available since

March 7, 2025

Availability

Academic Institutions and Nonprofits only

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pTIR_Poetur_RFP

Plasmid

#248202

Purpose

Poetur RFP cargo plasmid

Depositor

Marc Güell

Publication

Ivancic et al Nat Biotechnol. 2025 Oct 2. doi: 10.1038

Insert

RFP

Expression

Mammalian

Available since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pPP378

Plasmid

#158797

Purpose

pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-1(D371A) in MCS1 for expression in E. coli and protein purification

Depositor

Jennifer Doudna

Publication

Pausch et al Science. 2020 Jul 17;369(6501):333-337.

Insert

dCasPhi-1 (D371A)

Expression

Bacterial

Available since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-MTOR-D2512H

Plasmid

#69017

Purpose

activating MTOR mutation

Depositor

David Sabatini

Publication

Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

Insert

MTOR-D2512H (MTOR Human)

Tags

FLAG

Expression

Mammalian

Mutation

change Aspartate 2512 to Histidine

Available since

Dec. 16, 2015

Availability

Academic Institutions and Nonprofits only
pRL014

Plasmid

#250165

Purpose

DGRec 2-plasmid system: encodes bRT and avd.

Depositor

David Bikard

Publication

Rochette et al Nat Biotechnol. 2026 Apr 16. doi: 10.103

Insert

bRT (brt Bordetella phage BPP-1)

Expression

Bacterial

Available since

Aug. 11, 2026

Availability

Academic Institutions and Nonprofits only

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pRL038

Plasmid

#250169

Purpose

DGRec 2 plasmid-system: encodes bRT, avd, and a dgrRNA cloning site (containing ccdB toxin, to replace with TR). Allows dual targeting in combination with pRL021.

Depositor

David Bikard

Publication

Rochette et al Nat Biotechnol. 2026 Apr 16. doi: 10.103

Insert

bRT (brt Bordetella phage BPP-1)

Expression

Bacterial

Available since

Aug. 11, 2026

Availability

Academic Institutions and Nonprofits only

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pPR150

Plasmid

#250170

Purpose

DGRec single plasmid system: encodes bRT, Avd, a dgrRNA cloning site, CspRecT and MutL*.

Depositor

David Bikard

Publication

Rochette et al Nat Biotechnol. 2026 Apr 16. doi: 10.103

Insert

bRT (brt Bordetella phage BPP-1)

Expression

Bacterial

Available since

Aug. 11, 2026

Availability

Academic Institutions and Nonprofits only

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pYPQ141-ZmUbi-RZ-Cas12j2

Plasmid

#173926

Purpose

Gateway entry clone (attL5 & attL2) for Cas12j2 crRNA expression under ZmUbi promoter with ribozyme processing

Depositor

Yiping Qi

Publication

Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pCDB181

Plasmid

#80692

Purpose

for secretion of N-terminal OsmY, deca-histidine, SUMO tagged gEHEE_06

Depositor

David Baker

Publication

Bhardwaj et al Nature. 2016 Sep 14. doi: 10.1038/nature

Insert

gEHEE_06

Tags

OsmY-10His-Smt3

Expression

Bacterial

Promoter

T7

Available since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shControl-EGFP

Plasmid

#227685

Purpose

Expresses EGFP and scramble of shRNA targeting Atg7

Depositor

Cagla Eroglu

Publication

Wang et al eLife 2025 [Reviewed Preprint] 14:RP1075

Insert

scrambled shRNA control

Expression

Mammalian

Available since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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SM420_pHAGE-CAG-mcherry-P2A-puro-DR-Lb_crRNA-TruSeq_3

Plasmid

#244224

Purpose

Vector for cloning LbCas12a crRNA array libraries. Contains 3' TruSeq sequence for easy NGS library construction

Depositor

Tim Reddy

Publication

Melore et al Nat Commun. 2026 Feb 12. doi: 10.1038/s4

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Jan. 5, 2026

Availability

Academic Institutions and Nonprofits only

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