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Showing: 281 - 300 of 300 results

  1. pTXB1-TP1

    Plasmid
    #160084
    Purpose
    Bacterial expression of Tn5-APEX2 fusion protein, no linker
    Depositor
    Pier Pandolfi
    Article
    Lee et al Nat Methods. 2021 Mar;18(3):293-302. doi
    Insert
    Tn5 transposase/APEX2 peroxidase fusion protein
    Use
    Tags
    FLAG and Mxe intein - Chitin-binding domain
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    160084-plasmid-map-sequence-id-314364

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  2. pTXB1-TP2

    Plasmid
    #160085
    Purpose
    Bacterial expression of Tn5-APEX2 fusion protein with linker PAPAP
    Depositor
    Pier Pandolfi
    Article
    Lee et al Nat Methods. 2021 Mar;18(3):293-302. doi
    Insert
    Tn5 transposase/APEX2 peroxidase fusion protein
    Use
    Tags
    FLAG and Mxe intein - Chitin-binding domain
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    160085-plasmid-map-sequence-id-313737

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  4. pLenti-RIGI-Apex- T2A-EGFP

    Plasmid
    #167288
    Purpose
    Lentiviral expression plasmid encoding an APEX2 RIG-I fusion product in frame with a self-cleaving eGFP. APEX biotin ligase can biotynilate RNAs in proximity of the RLR RIG-I sensor.
    Depositor
    Agnel Sfeir
    Article
    Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:
    Insert
    DDX58 (RIGI Human)
    Use
    Lentiviral
    Tags
    APEX2 and EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    167288-plasmid-map-sequence-id-330940

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  5. Inducible Caspex expression

    Plasmid
    #97421
    Purpose
    For stable clone generation of cells expressing the dCas9-APEX2 fusion protein for Genomic locus proteomics
    Depositor
    Steven Carr, Samuel Myers
    Article
    Myers et al Nat Methods. 2018 May 7. pii: 10.1038/s4
    Inserts
    Caspex
    T2A-Gfp
    Use
    CRISPR
    Tags
    3xFLAG and V5
    Expression
    Mammalian
    Mutation
    dCas9: D10A and H840A, APEX2: K14D, W41F, E112K, …
    Promoter
    TRE3G
    Available since
    Oct. 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    97421-plasmid-map-sequence-id-466895

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  8. Anti-TRIP8b [N212/17R-rat IgG2a] - Chimeric

    Plasmid
    #231833
    Purpose
    Mammalian expression plasmid of anti-TRIP8b (Rat) rat IgG2a R-mab. Derived from hybridoma N212/17.
    Depositor
    James Trimmer
    Article
    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1
    Insert
    Anti-TRIP8b (Rattus norvegicus) recombinant mouse monoclonal antibody (Pex5l Chimera: M. musculus (mouse)/R. norvegicus (rat))
    Use
    Affinity Reagent/ Antibody
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Dual CMV
    Available since
    Feb. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    231833-plasmid-map-sequence-id-459976

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  9. Anti-TRIP8b (exon 1a-5) [N291A/43R]

    Plasmid
    #225389
    Purpose
    Mammalian Expression Plasmid of anti-TRIP8b (exon 1a-5) (Mouse) IgG2a R-mAb. Derived from hybridoma N291A/43.
    Depositor
    James Trimmer
    Article
    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1
    Insert
    Anti-TRIP8b (exon 1a-5) (Mus musculus) recombinant (Mouse) monoclonal antibody. (Pex5l Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Dual CMV
    Available since
    Oct. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    225389-plasmid-map-sequence-id-449185

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  10. Anti-TRIP8b (constant) ion channel [N212/27R]

    Plasmid
    #220409
    Purpose
    Mammalian Expression Plasmid of anti-TRIP8b (constant) ion channel (Rat) IgG2a R-mAb. Derived from hybridoma N212/27.
    Depositor
    James Trimmer
    Article
    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1
    Insert
    Anti-TRIP8b (constant) ion channel (Rattus novegicus) recombinant mouse monoclonal antibody. (Pex5l Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Dual CMV
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    220409-plasmid-map-sequence-id-435565

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  15. SZ_EC_02

    Plasmid
    #155341
    Purpose
    Overexpression of xylose utilization genes of Yarrowia lipolytica using EasyCloneYALI. Cut with NotI to linearize repair fragment for C3 locus, overexpressing XK, XDH, and XR
    Depositor
    Jens Nielsen
    Article
    Konzock et al BMC Microbiol. 2021 Mar 8;21(1):77. doi:
    Inserts
    tef1 promoter - xylulose kinase - pex20 terminator
    pyk1 promoter - xylose reductase - pex16 terminator
    gapdh promoter - xylitol dehydrogenase - lip2 terminator
    Use
    Easycloneyali insert vector
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    gapdh, pyk1, and tef1
    Available since
    March 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    155341-plasmid-map-sequence-id-316921

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  16. pUC57 APEX

    Plasmid
    #40306
    Purpose
    A monomeric and improved-activity variant of pea ascorbate peroxidase. Used for electron microscopy and live-cell proteomic mapping. This construct has been replaced by APEX2 (construct 49386).
    Depositor
    Alice Ting
    Article
    Martell et al Nat Biotechnol. 2012 Oct 21. doi: 10.103
    Insert
    pea APEX (Synthetic)
    Use
    Unspecified
    Tags
    Expression
    Mutation
    K14D, W41F, E112K (relative to wild-type)
    Promoter
    Available since
    Sept. 5, 2012
    Availability
    Academic Institutions and Nonprofits only
    40306-plasmid-map-sequence-id-115990

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  19. pMVP and pMAGIC Cloning Systems

    Plasmid Kit
    #1000000155
    Purpose
    pMVP and pMAGIC are cloning systems for adenoviral, lentiviral, expression, PiggyBac transposon, and Sleeping Beauty transposon vectors for transgene or RNAi delivery and dCas9-based engineering.
    Depositor
    Christopher Newgard
    Available since
    Sept. 6, 2019
    Availability
    Academic Institutions and Nonprofits only
Showing: 281 - 300 of 300 results