We narrowed to 9,861 results for: plasmids 101

1. pCJH027_SC101_pLAC_LbCas12a_Kan

   Plasmid

   #183074

   Purpose

   Wild-type LbCas12a (Chen et al., 2018) in a plasmid containing a SC101 origin and lac promoter for in vivo interference assay.

   Depositor

   Jennifer Doudna

   Article

   Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

   Insert

   LbCas12a
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Wild-type

   Promoter

   Available since

   June 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFH101

   Plasmid

   #128206

   Purpose

   sgRNA backbone for StCas9, combine with AtU6-26 promoter module (pFH35)

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   sgRNA backbone for StCas9, combine with AtU6-26 promoter module (pFH35)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHL1017

   Plasmid

   #62828

   Purpose

   Lim Lab plasmid: AmpR + PConNoHindM8::glgC::gfp::T1T2 terminator + ColE1

   Depositor

   Han Lim

   Article

   Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv

   Insert

   gfp
   

   Use

   Synthetic Biology

   Tags

   m8::glgC leader

   Expression

   Bacterial

   Mutation

   Promoter

   pConNoHind

   Available since

   Aug. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pCJH019_SC101_pLAC_Cas12c(4)_Kan

   Plasmid

   #183071

   Purpose

   Wild-type Cas12c_4 in a plasmid containing a SC101 origin and lac promoter for in vivo interference assay.

   Depositor

   Jennifer Doudna

   Article

   Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

   Insert

   Cas12c_4
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Wild-type

   Promoter

   Available since

   June 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTL101

   Plasmid

   #168335

   Purpose

   pGBKT7 yeast two-hybrid plasmid

   Depositor

   Sophien Kamoun

   Article

   Bentham et al PLoS Pathog. 2021 Nov 10;17(11):e1009957

   Insert

   Si514806415
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSC101_TIMER

   Plasmid

   #103057

   Purpose

   low copy plasmid for expression of a variant of the fluorescent timer protein suitable for bacteria under the control of the constitutive ybaJp promoter (works in E. coli and Salmonella enterica)

   Depositor

   Dirk Bumann

   Article

   Claudi et al Cell. 2014 Aug 14;158(4):722-733. doi: 1

   Insert

   TIMER-bac
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   mutations: S4T and S179T

   Promoter

   constitutive ybaJp promoter

   Available since

   Nov. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGAN101

   Plasmid

   #131123

   Purpose

   Plasmid for overexpression of prion domains to induce prions

   Depositor

   Ahmad Khalil

   Article

   Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

   Type

   Empty backbone

   Use

   Tags

   mKate2

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBTK1017 (pNR)

   Plasmid

   #183131

   Purpose

   Control plasmid that does not express dsRNA

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al Science. 2020 Jan 31;367(6477):573-576.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pXTK023 [Origin_pSC101hs_PartType1]

   Plasmid

   #229260

   Purpose

   XanthoMoClo Golden Gate Part Plasmid for cloning, contains Origin_pSC101hs_PartType1

   Depositor

   Michael Springer

   Article

   Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

   Insert

   pSC101hs Origin
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pXTK024 [Origin_RSF1010_PartType1]

    Plasmid

    #229261

    Purpose

    XanthoMoClo Golden Gate Part Plasmid for cloning, contains Origin_RSF1010_PartType1

    Depositor

    Michael Springer

    Article

    Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

    Insert

    RSF1010 Origin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRRG101-mblk

    Plasmid

    #99091

    Purpose

    dsRNA and riboprobe synthesis for Schmidtea mediterranea mblk

    Depositor

    Phillip Newmark

    Article

    Roberts-Galbraith et al Elife. 2016 Sep 9;5. pii: e17002. doi: 1

    Insert

    mblk in situ probe
    

    Use

    T/a cloning vector for dsrna generation and the g…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. KL101: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS-KRAB

    Plasmid

    #121837

    Purpose

    pMAGIC R4-R3 entry plasmid, contains 2x NLS/KRAB scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sites

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. TAL3101

    Plasmid

    #41275

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    ZebrafishCommunity-id2a-Right (id2a Zebrafish)
    

    Use

    t7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pRS314-prp16-101

    Plasmid

    #89973

    Purpose

    prp16-101 allele in TRP plasmid

    Depositor

    Christine Guthrie

    Article

    Mayerle et al RNA. 2016 May;22(5):793-809. doi: 10.126

    Insert

    PRP16 (PRP16 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    prp16-101 allele (G373S)

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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15. KO101: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS-Dnmt3a3L

    Plasmid

    #121833

    Purpose

    pMAGIC R4-R3 entry plasmid, contains 2x NLS x-dCas9(3.7) fused to the Dnmt3a-3L DNA methyltransferase for 3 or 4-component MultiSite Gateway Pro assembly.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    x-dCas9(3.7)/Dnmt3a-3L (open)
    

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    NLS

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pXTK101 [Term_T0_PartType7]

    Plasmid

    #229338

    Purpose

    XanthoMoClo Golden Gate Part Plasmid for cloning, contains Term_T0_PartType7

    Depositor

    Michael Springer

    Article

    Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

    Insert

    T0 Terminator
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRRG1014-LDLRR-3

    Plasmid

    #99095

    Purpose

    dsRNA and riboprobe synthesis for Schmidtea mediterranea LDLRR-3

    Depositor

    Phillip Newmark

    Article

    Roberts-Galbraith et al Elife. 2016 Sep 9;5. pii: e17002. doi: 1

    Insert

    LDLRR-3 in situ probe
    

    Use

    T/a cloning vector for dsrna generation and the g…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRRG1013-LDLRR-1

    Plasmid

    #99093

    Purpose

    dsRNA and riboprobe synthesis for Schmidtea mediterranea LDLRR-1

    Depositor

    Phillip Newmark

    Article

    Roberts-Galbraith et al Elife. 2016 Sep 9;5. pii: e17002. doi: 1

    Insert

    LDLRR-1 in situ probe
    

    Use

    T/a cloning vector for dsrna generation and the g…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pc3XB-ZF101

    Plasmid

    #13235

    Depositor

    Daniel Voytas

    Article

    Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

    Insert

    ZF101
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 31, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET26b_Nb.AD101

    Plasmid

    #131402

    Purpose

    E. coli periplasmic expression of conformational nanobody Nb.AD101 recognizing purified active-state A2a adenosine receptor

    Depositor

    Andrew Kruse

    Article

    McMahon et al Nat Struct Mol Biol. 2018 Mar;25(3):289-

    Insert

    Nanobody AD101
    

    Use

    Tags

    6xHis-tag and pelB leader

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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