We narrowed to 9,255 results for: yeast expression

1. pCas9

   Plasmid

   #162606

   Purpose

   Pre-express Cas9 in yeast

   Depositor

   Lars Steinmetz

   Article

   Sankaran et al J Microbiol Biol Educ. 2021 May 31;22(2)

   Insert

   Tef1-Cas9
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. 3xFLAG-dCas9/pTEF1p-CYC1t

   Plasmid

   #62190

   Purpose

   Expresses 3xFLAG-dCas9 in budding yeast for enChIP analysis to purify specific genomic regions of interest.

   Depositor

   Hodaka Fujii

   Article

   Fujii et al Biol Methods Protoc. 2022 Oct 17;7(1):bp

   Insert

   3xFLAG-dCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Yeast

   Mutation

   human codon-optimized, D10A, H840A

   Promoter

   TEF1 promoter

   Available since

   Feb. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAH237

   Plasmid

   #121146

   Purpose

   Expression of both nmt41p-cas9 and rrk1p-gRNA (LEU2 marker) for CRISPR genome editing in fission yeast

   Depositor

   Katsunori Tanaka

   Article

   Hayashi et al G3 (Bethesda). 2019 Feb 12. pii: g3.118.

   Inserts

   gRNA cassette
   humanized Streptococcus pyogenes Cas9
   

   Use

   CRISPR

   Tags

   Flag

   Expression

   Yeast

   Mutation

   Promoter

   S.pombe rrk1 and nmt41

   Available since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGBW-m4046663

   Plasmid

   #153857

   Purpose

   Yeast expression plasmid for SARS-CoV-2 nsp3

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 nsp3 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pGAL1

   Available since

   June 12, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pGBW-m4046672

   Plasmid

   #153918

   Purpose

   Yeast expression plasmid for SARS-CoV-2 nsp2

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 nsp2 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pGAL1

   Available since

   June 12, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pGBW-m4046632

   Plasmid

   #153929

   Purpose

   Yeast expression plasmid for SARS-CoV-2 nsp3

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 nsp3 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pTEF1

   Available since

   June 12, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pGBW-m4046697

   Plasmid

   #153869

   Purpose

   Yeast expression plasmid for SARS-CoV-2 nsp4

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 nsp4 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pGAL1

   Available since

   June 12, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pGBW-m4046627

   Plasmid

   #153916

   Purpose

   Yeast expression plasmid for SARS-CoV-2 nsp7

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 nsp7 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pTEF1

   Available since

   June 12, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pGBW-m4046638

   Plasmid

   #153941

   Purpose

   Yeast expression plasmid for SARS-CoV-2 helicase

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 helicase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pTEF1

   Available since

   June 12, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pGBW-m4046622

    Plasmid

    #153904

    Purpose

    Yeast expression plasmid for SARS-CoV-2 helicase

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 helicase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pGAL1

    Available since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pGBW-m4046635

    Plasmid

    #149149

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 nsp9

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp9 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pGAL1

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pAG25-1199 cDNA

    Plasmid

    #160379

    Purpose

    N1199 narnavirus cDNA

    Depositor

    John McCusker

    Insert

    N1199
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    NA

    Available since

    Dec. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTIN7m

    Plasmid

    #162886

    Purpose

    Overexpression vector for the fission yeast Schizosaccharomyces pombe (lower strength)

    Depositor

    Stewart Shuman

    Article

    Garg et al RNA. 2020 Nov;26(11):1743-1752. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGBK CUP1 mGrape3

    Plasmid

    #170265

    Purpose

    Yeast two-hybrid vector using a copper inducible CUP1 promoter, expressing mGrape3

    Depositor

    Bruce Hamilton

    Article

    Hernandez et al bioRxiv

    Insert

    momeric Grape 3
    

    Use

    Tags

    GAL4 DNA Binding Domain and Myc

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    CUP1

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGBK CUP1 EGFP

    Plasmid

    #170210

    Purpose

    Yeast two-hybrid vector using a copper inducible CUP1 promoter, expressing EGFP

    Depositor

    Bruce Hamilton

    Article

    Hernandez et al bioRxiv

    Insert

    enhanced green fluorescent protein
    

    Use

    Tags

    GAL4 DNA Binding Domain and Myc

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    CUP1

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAH243

    Plasmid

    #121147

    Purpose

    Expression of both nmt41p-cas9 and rrk1p-gRNA (ura4 marker) for CRISPR genome editing in fission yeast

    Depositor

    Katsunori Tanaka

    Article

    Hayashi et al G3 (Bethesda). 2019 Feb 12. pii: g3.118.

    Inserts

    humanized Streptococcus pyogenes Cas9
    gRNA expression module
    

    Use

    CRISPR

    Tags

    Flag

    Expression

    Yeast

    Mutation

    Promoter

    S.pombe rrk1 and nmt41

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pETcon(-)

    Plasmid

    #41522

    Depositor

    Andrew Scharenberg

    Type

    Empty backbone

    Use

    Tags

    3x(G4S) Linker, G3S Linker, HA, Myc Tag, and Stuf…

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    April 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. FRP935

    Plasmid

    #159302

    Purpose

    Yeast tagging vector to create gene fusion with yeast codon optimized mAmetrine under KanMX selection

    Depositor

    Joerg Stelling

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. FRP1999

    Plasmid

    #159308

    Purpose

    Yeast tagging vector to create gene fusion with yeast codon optimized mCardinal under KanMX selection

    Depositor

    Joerg Stelling

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. FRP2000

    Plasmid

    #159309

    Purpose

    Yeast tagging vector to create gene fusion with yeast codon optimized mNeptune2 under KanMX selection

    Depositor

    Joerg Stelling

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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