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pTORGm43GWY

Plasmid

#112906

Purpose

Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to gentamicin. Contains additional cassette for constitutive expression of mCitrine.

Depositor

Sebastian Schornack

Article

Evangelisti et al BMC Microbiol. 2019 Nov 27;19(1):265. do

Type

Empty backbone

Use

Oomycete expression

Available Since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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pC4M-F2E-GFP-FKBP

Plasmid

#68058

Purpose

express EGFP-tagged FKBP for heterodimerization with FRB in mammalian cells

Depositor

Richard Youle

Article

Narendra et al Autophagy. 2010 Nov;6(8):1090-106.

Insert

EGFP

Tags

2xFKBP and HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 9, 2015

Availability

Academic Institutions and Nonprofits only

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Thy1-Brainbow-2.1 R

Plasmid

#18727

Depositor

Joshua Sanes

Article

Livet et al Nature. 2007 Nov 1. 450(7166):56-62.

Insert

hrGFPII-NLS ; EYFP ; tdimer2 ; M-mCerulean

Use

Cre/Lox and Mouse Targeting

Expression

Mammalian

Available Since

Aug. 15, 2008

Availability

Academic Institutions and Nonprofits only

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pUC-UAS1B8-Leum

Plasmid

#44316

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B8-Leum promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B8-Leum

Available Since

April 18, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pUC-UAS1B16-TEF(504)

Plasmid

#44371

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B16-TEF(504) promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B16-TEF(504)

Available Since

July 15, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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p8267 LentiCRISPR v2 hygro sgNT-2

Plasmid

#193978

Purpose

Expression of spCas9 and non-targeting control sgRNA

Depositor

Elizabeth White

Article

Castagnino et al J Virol. 2022 Nov 7:e0132622. doi: 10.11

Insert

spCas9

Use

Lentiviral

Available Since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pJK226

Plasmid

#71125

Purpose

Expression of Acetobacter aceti 1023 citrate synthase (AaCS) in E. coli

Depositor

T. J. Kappock

Article

Francois et al Biochemistry. 2006 Nov 14;45(45):13487-9

Insert

citrate synthase

Expression

Bacterial

Promoter

T7

Available Since

Jan. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pTC150

Plasmid

#70014

Purpose

'Donor only' control plasmid for gene targeting of tomato ANT1 gene. Contains donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon

Depositor

Daniel Voytas

Article

Cermak et al Genome Biol. 2015 Nov 6;16(1):232. doi:

Insert

Donor + GVR

Expression

Plant

Available Since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pBbE1k-DDC

Plasmid

#71723

Purpose

dopa decarboxylase from pig

Depositor

Taek Soon Lee

Article

Satoh et al Metab Eng. 2012 Nov;14(6):603-10. doi: 1

Insert

dopa decarboxylase

Promoter

pTrc

Available Since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pGS403_scFv-GCN4-sfGFP-GB1-HRV3C-CENP-T[1-242 T11A, T85A] in AAVS donor

Plasmid

#211888

Purpose

CENP-T[1-242] transgene integration at AAVS1 safe harbor locus

Depositor

Iain Cheeseman

Insert

CENP-T[1-242] (CENPT Synthetic, Human)

Use

CRISPR

Mutation

T11A, T85A

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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CMV-HA-hTAF5

Plasmid

#17984

Depositor

Naoko Tanese

Article

Tanese et al Proc Natl Acad Sci U S A. 1996 Nov 26. 9

Insert

TAF5 (TAF5 Human)

Tags

HA

Expression

Mammalian

Available Since

Aug. 19, 2008

Availability

Academic Institutions and Nonprofits only
pFLAG-Fbw7α-3'UTR

Plasmid

#26650

Depositor

Alex Minella

Article

Xu et al J Biol Chem. 2010 Nov 5;285(45):34439-46

Insert

Fbw7α

Tags

3XFLAG

Expression

Mammalian

Available Since

Oct. 25, 2012

Availability

Academic Institutions and Nonprofits only

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Tubulin-mhYFP

Plasmid

#186525

Purpose

Localization of monomeric hyperfolder YFP fluorescent protein to tubulin

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

Monomeric hyperfolder YFP

Tags

Tubulin

Expression

Mammalian

Mutation

hfYFP-S147P/L195M/V206K

Promoter

CMV

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pGAL4-CREB

Plasmid

#15221

Depositor

Ugo Moens

Article

Delghandi et al Cell Signal. 2005 Nov . 17(11):1343-51.

Insert

CREB (Creb1 Rat)

Tags

Gal4

Expression

Mammalian

Available Since

June 29, 2007

Availability

Academic Institutions and Nonprofits only
pOPINVL

Plasmid

#26040

Depositor

Ray Owens

Article

Nettleship et al Protein Expr Purif. 2008 Nov . 62(1):83-

Insert

u-LacZ

Tags

u-phosphatase secretion leader

Expression

Bacterial, Insect, and Mamm…

Available Since

June 30, 2011

Availability

Academic Institutions and Nonprofits only

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pCFD3-frame_selector_0

Plasmid

#127553

Purpose

Drosophila expression of frame selector sgRNA 0 that targets CRISPaint site for homology-independent knock-in

Depositor

Norbert Perrimon

Article

Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

Insert

sgRNA frame 0

Use

CRISPR

Expression

Insect

Promoter

dU6:3

Available Since

July 16, 2019

Availability

Academic Institutions and Nonprofits only

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pOPINVH

Plasmid

#26041

Depositor

Ray Owens

Article

Nettleship et al Protein Expr Purif. 2008 Nov . 62(1):83-

Insert

u-LacZ-KHHHHHH

Tags

KHHHHHH and u-phosphatase secretion leader

Expression

Bacterial, Insect, and Mamm…

Available Since

June 30, 2011

Availability

Academic Institutions and Nonprofits only

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pUC-UAS1B16-TEF(272)

Plasmid

#44375

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B16-TEF(272) promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B16-TEF(272)

Available Since

April 18, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pET-gSTEP1

Plasmid

#159376

Purpose

Expresses gSTEP1 in E. coli

Depositor

Roberto Chica

Article

Eason et al ACS Synth Biol. 2020 Nov 20;9(11):2955-2

Insert

gSTEP1

Tags

6x His tag

Expression

Bacterial

Promoter

T7

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO-FLAG-MORF4L1(iso2)

Plasmid

#86874

Purpose

To express MORF4L1 (iso2) with a FLAG tag at N-terminus.

Depositor

Yihong Ye

Article

Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

Insert

MORF4L1 (MORF4L1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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