We narrowed to 6,266 results for: 1-Oct;

1. ATP1A1_G3_MTOR_Nick_Exon-53_Dual_sgRNA

   Plasmid

   #173211

   Purpose

   Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G3 and MTOR Nick exon-53 sgRNAs from two independent U6 promoters.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1 G3 sgRNA + MTOR Nick exon-53 sgRNA
   

   Use

   CRISPR; Prime editing (pe3)

   Expression

   Mammalian

   Promoter

   Tandem U6 promoters

   Available Since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. ATP1A1_G4_Q118R_RUNX1_+4_T_to_G_Dual_pegRNA

   Plasmid

   #173212

   Purpose

   Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 and RUNX1 +4 T to G pegRNAs from two independent U6 promoters.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1 G4 Q118R pegRNA + RUNX1 +4 T to G pegRNA
   

   Use

   CRISPR; Prime editing

   Expression

   Mammalian

   Promoter

   Tandem U6 promoters

   Available Since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEM-HE-bPAC(R278A)-Myc-eYFP

   Plasmid

   #165488

   Purpose

   Xenopus oocyte expression of soluble photoactivatable adenylyl cyclase derived from bPAC with reduced dark activity

   Depositor

   Thomas Oertner

   Article

   Yang et al BMC Biol. 2021 Oct 18;19(1):227. doi: 10

   Insert

   bPAC(R278A)-Myc-eYFP
   

   Use

   Cdna expression, xenopus oocyte

   Tags

   EYFP and Myc

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pIRES-EGFP-TPD53

   Plasmid

   #172461

   Purpose

   Expression of untagged Tumor Protein D52 like 1 (TPD53/TPD52L1) and GFP

   Depositor

   Stephen Royle

   Article

   Larocque et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

   Insert

   TPD53 (TPD52L1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLM033

   Plasmid

   #134245

   Purpose

   Retroviral plasmid with H4 EGFP insert

   Depositor

   Iain Cheeseman

   Article

   Swartz et al Dev Cell. 2019 Oct 7;51(1):35-48.e7. doi

   Insert

   Human H4 + eGFP/HALO retroviral tagging (HRH4 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   March 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. NH2end ELL2

   Plasmid

   #127252

   Purpose

   Expresses the N terminal segment of human ELL2 in mammalian cells

   Depositor

   Christine Milcarek

   Article

   Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

   Insert

   ELL2 (1-287) (ELL2 Human)
   

   Use

   Other

   Tags

   His6X

   Expression

   Mammalian

   Mutation

   contains only amino acids 1-289

   Promoter

   pEF1a

   Available Since

   July 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. Gal4-TET1-878-911-I894A(pL84)

   Plasmid

   #124404

   Purpose

   Gal4/VP16 luciferase reporter assay. Gal4 DBD fusion of TET1 SID with I894A mutation.

   Depositor

   Shaun Cowley

   Article

   Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

   Insert

   Gal4-TET1-878-911(I894A mutation) (TET1 Human)
   

   Tags

   gal4 dna binding domain

   Expression

   Mammalian

   Mutation

   I894A

   Available Since

   May 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. Gal4-TET1-878-911-T898A(pL84)

   Plasmid

   #124403

   Purpose

   Gal4/VP16 luciferase reporter assay. Gal4 DBD fusion of TET1 SID with T898A mutation.

   Depositor

   Shaun Cowley

   Article

   Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

   Insert

   Gal4-TET1-878-911(T898A mutation) (TET1 Human)
   

   Tags

   gal4 dna binding domain

   Expression

   Mammalian

   Mutation

   T898A

   Available Since

   May 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. Gal4-TET1-878-911-L900A(pL84)

   Plasmid

   #124402

   Purpose

   Gal4/VP16 luciferase reporter assay. Gal4 DBD fusion of TET1 SID with L900A mutation.

   Depositor

   Shaun Cowley

   Article

   Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

   Insert

   Gal4-TET1-878-911(L900A mutation) (TET1 Human)
   

   Tags

   gal4 dna binding domain

   Expression

   Mammalian

   Mutation

   L900A

   Available Since

   May 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. Gal4-TET1-878-911-L897A(pL84)

    Plasmid

    #124401

    Purpose

    Gal4/VP16 luciferase reporter assay. Gal4 DBD fusion of TET1 SID with L897A mutation.

    Depositor

    Shaun Cowley

    Article

    Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

    Insert

    Gal4-TET1-878-911(L897A mutation) (TET1 Human)
    

    Tags

    gal4 dna binding domain

    Expression

    Mammalian

    Mutation

    L897A

    Available Since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. GB1-His-TET1-878-911-L900A(pL91)

    Plasmid

    #124395

    Purpose

    E.Coli produced TET1-SID mutants (L900A) purified by FPLC nickel column

    Depositor

    Shaun Cowley

    Article

    Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

    Insert

    GB1-His-TET1-878-911-L900A (TET1 Human)
    

    Tags

    GB1-Histidine

    Expression

    Bacterial

    Mutation

    L900A

    Available Since

    May 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. GB1-His-TET1-878-911-T898E(pL91)

    Plasmid

    #124394

    Purpose

    E.Coli produced TET1-SID mutants (T898E) purified by FPLC nickel column

    Depositor

    Shaun Cowley

    Article

    Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

    Insert

    GB1-His-TET1-878-911-T898E (TET1 Human)
    

    Tags

    GB1-Histidine

    Expression

    Bacterial

    Mutation

    T898E

    Available Since

    May 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. GB1-His-TET1-878-911-T898A(pL91)

    Plasmid

    #124393

    Purpose

    E.Coli produced TET1-SID mutants (T898A) purified by FPLC nickel column

    Depositor

    Shaun Cowley

    Article

    Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

    Insert

    GB1-His-TET1-878-911-T898A (TET1 Human)
    

    Tags

    GB1-Histidine

    Expression

    Bacterial

    Mutation

    T898A

    Available Since

    May 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. GB1-His-TET1-878-911-L897A(pL91)

    Plasmid

    #124392

    Purpose

    E.Coli produced TET1-SID mutants (L897A) purified by FPLC nickel column

    Depositor

    Shaun Cowley

    Article

    Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

    Insert

    GB1-His-TET1-878-911-L897A (TET1 Human)
    

    Tags

    GB1-Histidine

    Expression

    Bacterial

    Mutation

    L897A

    Available Since

    May 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. GB1-His-TET1-878-911-I894A(pL91)

    Plasmid

    #124391

    Purpose

    E.Coli produced TET1-SID mutants (I894A) purified by FPLC nickel column

    Depositor

    Shaun Cowley

    Article

    Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

    Insert

    GB1-His-TET1-878-911-I894A (TET1 Human)
    

    Tags

    GB1-Histidine

    Expression

    Bacterial

    Mutation

    I894A

    Available Since

    May 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPICZ-pOst1-pro-alphaf(MUT2)-E2-Crimson

    Plasmid

    #117663

    Purpose

    Test intracellular localisation of E2-Crimson and study the secretion efficiency

    Depositor

    Pau Ferrer, Benjamin Glick

    Article

    Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

    Insert

    pOst1-pro-af(MUT2)-E2-Crimson
    

    Expression

    Yeast

    Mutation

    This plasmid encodes the fluorescent protein E2-C…

    Promoter

    pAOX1

    Available Since

    Dec. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. HeFm1SpCas9

    Plasmid

    #92110

    Purpose

    Expression plasmid for human codon-optimized increased fidelity HeFm1SpCas9 (without U6-sgRNA coding sequence)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    3xFLAG-NLS-Streptococcus pyogenes Highly enhanced Fidelity mut1 Cas9-NLS
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    R661A, Q695A, K848A, Q926A, K1003A, R1060A

    Promoter

    Cbh

    Available Since

    Oct. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. GAL-ARF

    Plasmid

    #89121

    Purpose

    mammalian expression of p14-hARF linked to GAL4 DNA binding domain

    Depositor

    Elizabeth Wilson

    Article

    Minges et al J Biol Chem. 2015 Oct 9;290(41):25174-87

    Insert

    p14-hARF (CDKN2A Human)
    

    Tags

    GAL4 DBD

    Expression

    Mammalian

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pmCherry_C1 I3(resist)

    Plasmid

    #61399

    Purpose

    Expresses mCherry tagged Inhibitor-3 resistant to I3 S2 siRNA in mammalian cells

    Depositor

    Hemmo Meyer

    Article

    Eiteneuer et al EMBO J. 2014 Oct 8. pii: e201489054.

    Insert

    Inhibitor-3 (PPP1R11 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    silent mutations of bases C183T T192C T189C

    Promoter

    CMV

    Available Since

    Sept. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA5FRT/TO-GFP-I3(resist)

    Plasmid

    #61400

    Purpose

    Expresses GFP-tagged Inhibitor-3 resistant to I3 S2 siRNA in mammalian cells

    Depositor

    Hemmo Meyer

    Article

    Eiteneuer et al EMBO J. 2014 Oct 8. pii: e201489054.

    Insert

    Inhibitor-3 (PPP1R11 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    silent mutations of bases C183T T192C T189C

    Promoter

    CMV

    Available Since

    Sept. 25, 2015

    Availability

    Academic Institutions and Nonprofits only