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plenti-arRNA-BFP-backbone

Plasmid

#180221

Purpose

For the cloning of arRNA-expressing vectors containing BFP fluorescence marker in mammalian cells

Depositor

Wensheng Wei

Article

Qu et al Nat Biotechnol. 2019 Sep;37(9):1059-1069

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-GI-ZFP2

Plasmid

#42215

Purpose

Expresses GI-ZFP2 for light-inducible activation of gene expression

Depositor

Charles Gersbach

Article

Polstein et al J Am Chem Soc. 2012 Oct 10;134(40):16480

Insert

GIGANTEA-FLAG-ZFP2 (GI Synthetic, Mustard Weed)

Tags

FLAG

Promoter

CMV

Available Since

Jan. 29, 2013

Availability

Academic Institutions and Nonprofits only

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FCK-Arch-CFP

Plasmid

#22221

Purpose

Lentiviral expression of Arch-CFP for optogenetic inhibition

Depositor

Edward Boyden

Article

Chow et al Nature. 2010 Jan 7. 463(7277):98-102.

Insert

Arch

Use

Lentiviral

Tags

CFP

Expression

Mammalian

Available Since

Jan. 6, 2010

Availability

Academic Institutions and Nonprofits only

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pENTR-L3-Csy4-Cas9n-EGFP-L2

Plasmid

#141046

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

Csy4-2A-Cas9n-2A-EGFP

Expression

Mammalian

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST-Cas9-tdTomato

Plasmid

#141013

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

Cas9

Expression

Mammalian

Available Since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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141_pT3TS_iCre

Plasmid

#171793

Purpose

Plasmid for optimised iCre RNA synthesis using T3 promoter.

Depositor

Jean Giacomotto

Article

Tromp et al Front Physiol. 2023 Aug 2;14:1221310. do

Insert

optimised Cre for RNA expression

Expression

Bacterial

Promoter

T3

Available Since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pOIMars1CyN

Plasmid

#75221

Purpose

Mammalian cell surface expression of fluorogen activating protein Mars1Cy

Depositor

Alan Waggoner

Article

Zhang et al J Clin Invest. 2015 Oct 1;125(10):3915-2

Insert

ss-HA-Mars1Cy-CD80(237-306)

Tags

HA epitope

Expression

Mammalian

Promoter

CMV

Available Since

May 31, 2016

Availability

Academic Institutions and Nonprofits only

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pHU6_chr12_FAM19A2-up-gRNA-for

Plasmid

#81221

Purpose

pHu6-gRNA-NT1_SpecR backbone, gRNA for targeting the upstream region of FAM19A2 locus

Depositor

David Liu

Article

Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

Insert

hU6 expression of gRNA

Expression

Mammalian

Promoter

U6

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pPB-DEST-R1R2-pNeo

Plasmid

#141057

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

Neomycin resistance gene

Expression

Mammalian

Available Since

July 7, 2020

Availability

Academic Institutions and Nonprofits only

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pMK1224

Plasmid

#84221

Purpose

Lentiviral expression of shRNAs, compatible with double-shRNA expression, SFFV promoter, BFP

Depositor

Martin Kampmann, Jonathan Weissman

Article

Kampmann et al Proc Natl Acad Sci U S A. 2015 Jun 30;11

Type

Empty backbone

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR2-L3-SfoI-EGFP-secNanoLuc-PBL-R1

Plasmid

#141025

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

EGFP

Expression

Mammalian

Available Since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-L1-PGK-Neo-L2

Plasmid

#141007

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

Neomycin resistance gene

Expression

Mammalian

Available Since

July 6, 2020

Availability

Academic Institutions and Nonprofits only

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pAB031a

Plasmid

#79221

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ HA4-Zif268; PlacIq lacI

Expression

Bacterial

Promoter

PlacZ; PlacIq

Available Since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR-L3-Cas9-L2

Plasmid

#141038

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

Cas9

Expression

Mammalian

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-L3-eSpCas9n(1.1)-2A-EGFP-L2

Plasmid

#141052

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

eSpCas9(1.1)-2A-EGFP

Expression

Mammalian

Available Since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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U6a

Plasmid

#117221

Purpose

Expresses gRNA in Aedes aegypti

Depositor

Omar Akbari

Article

Li et al Elife. 2020 Jan 21;9. pii: 51701. doi: 1

Insert

HR U6a promoter-gRNA

Use

Synthetic Biology

Expression

Insect

Promoter

U6

Available Since

Jan. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pKH032

Plasmid

#224548

Purpose

2A-3xNLS-2xClover mouse Car2 C-term targeting vector

Depositor

Elphege Nora

Article

Hansen et al Science. 2025 Nov 27:eadt4221. doi: 10.1

Insert

2xClover

Use

Mouse Targeting

Available Since

Dec. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pKH040

Plasmid

#224549

Purpose

2A-3xNLS-tdTomato mouse Car2 C-term targeting vector

Depositor

Elphege Nora

Article

Hansen et al Science. 2025 Nov 27:eadt4221. doi: 10.1

Insert

tdTomato

Use

CRISPR and Mouse Targeting

Available Since

Dec. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-Flag-mGSDMA2

Plasmid

#218941

Purpose

Expression of N-terminal FLAG tagged mouse gasdermin A2

Depositor

Christopher LaRock

Article

LaRock et al Nature. 2022 May;605(7910):527-531. doi:

Insert

mouse gsdma2 (Gsdma2 Mouse)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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Halo-ATG2A HRD

Plasmid

#207540

Purpose

Homologous recombination donor to integrate a 3xFLAG-HaloTag at the endogenous ATG2A locus in human cells

Depositor

Jens Schmidt

Article

Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

Insert

HaloTag flanked by human ATG2A locus sequences

Tags

HaloTag

Expression

Mammalian

Promoter

Endogenous

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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