We narrowed to 5,155 results for: PEP

1. pcDNA3-sACE2-T92Q-Fc(IgA1)

   Plasmid

   #145162

   Purpose

   Mammalian expression plasmid for soluble ACE2-Fc fusion (IgA1) glycosylation mutant T92Q

   Depositor

   Erik Procko

   Article

   Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

   Insert

   Angiotensin-converting enzyme 2 (ACE2 Human)
   

   Use

   Tags

   Fc region of human IgA1

   Expression

   Mammalian

   Mutation

   Extracellular, soluble protease domain (a.a. M1-D…

   Promoter

   CMV

   Available since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3-sACE2v3

   Plasmid

   #145150

   Purpose

   Mammalian expression plasmid for soluble ACE2 (protease domain) high affinity variant 3

   Depositor

   Erik Procko

   Article

   Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

   Insert

   Angiotensin-converting enzyme 2 (ACE2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Extracellular, soluble protease domain (a.a. M1-D…

   Promoter

   CMV

   Available since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3-sACE2v4-Fc(IgA1)

   Plasmid

   #145159

   Purpose

   Mammalian expression plasmid for soluble ACE2-Fc fusion (IgA1) high affinity variant 4

   Depositor

   Erik Procko

   Article

   Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

   Insert

   Angiotensin-converting enzyme 2 (ACE2 Human)
   

   Use

   Tags

   Fc region of human IgA1

   Expression

   Mammalian

   Mutation

   Extracellular, soluble protease domain (a.a. M1-D…

   Promoter

   CMV

   Available since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3-sACE2v4

   Plasmid

   #145151

   Purpose

   Mammalian expression plasmid for soluble ACE2 (protease domain) high affinity variant 4

   Depositor

   Erik Procko

   Article

   Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

   Insert

   Angiotensin-converting enzyme 2 (ACE2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Extracellular, soluble protease domain (a.a. M1-D…

   Promoter

   CMV

   Available since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3-sACE2v6

   Plasmid

   #145153

   Purpose

   Mammalian expression plasmid for soluble ACE2 (protease domain) high affinity variant 6

   Depositor

   Erik Procko

   Article

   Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

   Insert

   Angiotensin-converting enzyme 2 (ACE2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Extracellular, soluble protease domain (a.a. M1-D…

   Promoter

   CMV

   Available since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pFP.E222

   Plasmid

   #138248

   Purpose

   Three input / three output logic circuit plasmid - Expresses different intein split ECFs from different inducible promoters

   Depositor

   Baojun Wang

   Article

   Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

   Inserts

   araC (araC )
   ECF20.N.(VDA).M86.N2
   Terminator
   SspGyrB.C2.(SAK).ECF16.C
   Terminator
   rhaS
   ECF17.N.(NPC).NrdJ-1.N2
   Terminator
   M86.C2.(SDL).ECF20.C
   Terminator
   luxR
   Terminator
   ECF16.N.(AGG).SspGyrB.N2
   Terminator
   NrdJ-1.C2.(SEI).ECF17.C
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23115, P(araBAD), P(lux2), P(rhaBAD), iJ23101, a…

   Available since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET His6 MBP TEV HDAC8_374

   Plasmid

   #122174

   Purpose

   Expresses a truncated construct of histone deacetylase 8 for crystallography bearing an N-terminal tobacco etch virus protease-cleavable hexahistidine-maltose binding protein tag

   Depositor

   David Christianson

   Article

   Porter et al ACS Chem Biol. 2017 Sep 15;12(9):2281-22

   Insert

   His6 MBP TEV HDAC8_374 (HDAC8 Human)
   

   Use

   Tags

   hexahistidine tag and tobacco etch virus protease…

   Expression

   Bacterial

   Mutation

   Residues Met1–Pro7 and H375-V377 have been delete…

   Promoter

   T7

   Available since

   March 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMSCVpuro-Mito-DsRed1

   Plasmid

   #87379

   Purpose

   Retroviral transfection of cells to express DsRed1 (Ex555, Em585) localising to the mitochondrial matrix via the transit peptide from human TXN2 (Thioredoxin).

   Depositor

   Björn Stork

   Article

   Bohler et al Cell Death Dis. 2018 Feb 19;9(3):286. do

   Insert

   TXN2 mitochondrial transit peptide (TXN2 Human)
   

   Use

   Retroviral

   Tags

   DsRed1

   Expression

   Mammalian

   Mutation

   First 60 aa only

   Promoter

   Retroviral 5' LTR promoter

   Available since

   June 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-sACE2v2.4(732)-IgG1

   Plasmid

   #154106

   Purpose

   Mammalian expression plasmid for dimeric soluble ACE2 high affinity variant 2.4 (IgG1-Fc fusion)

   Depositor

   Erik Procko

   Article

   Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

   Insert

   Angiotensin-converting enzyme 2 (ACE2 Human)
   

   Use

   Tags

   Fc region of human IgG1

   Expression

   Mammalian

   Mutation

   Extracellular, soluble protease and neck domains …

   Promoter

   CMV

   Available since

   June 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pOTTC293 - pAAV EF1a V5-synuclein (WT)

    Plasmid

    #60057

    Purpose

    An AAV packaging vector that expresses wildtype alpha-synuclein under control of the EF1a promoter.

    Depositor

    Brandon Harvey

    Article

    Savolainen et al Neurobiol Dis. 2014 Aug;68:1-15. doi: 10

    Insert

    alpha-synuclein (SNCA Human, human)
    

    Use

    AAV

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3-sACE2v2.4(732)-8h

    Plasmid

    #154103

    Purpose

    Mammalian expression plasmid for dimeric soluble ACE2 high affinity variant 2.4 (8his tag)

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Use

    Tags

    Short GSG linker and 8his tag

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease domain (a.a. M1-G…

    Promoter

    CMV

    Available since

    June 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. 8024 GFP_Kras_G12C_2A_puro

    Plasmid

    #64373

    Purpose

    This a retroviral expression plasmid expressing GFP tagged G12C mutant Kras 2A oncogene along with puro resistance gene

    Depositor

    Georgios Stathopoulos

    Article

    Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

    Insert

    mouse Kras2A (Kras Mouse)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mutation

    changed glycine 12 to cysteine

    Promoter

    Available since

    July 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. 8027_GFP-KrasG12C_2B_puro

    Plasmid

    #64372

    Purpose

    This a retroviral expression plasmid expressing GFP tagged G12C mutant Kras 2B oncogene along with puro resistance gene

    Depositor

    Georgios Stathopoulos

    Article

    Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

    Insert

    mouse Kras2B (Kras Mouse)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mutation

    changed glycine 12 to cysteine

    Promoter

    Available since

    July 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. 8798 GFP-KrasG12C_2B_hygro

    Plasmid

    #64376

    Purpose

    This a retroviral expression plasmid expressing GFP tagged G12C mutant Kras 2B oncogene along with hygro resistance gene

    Depositor

    Georgios Stathopoulos

    Article

    Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

    Insert

    mouse Kras2B (Kras Mouse)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mutation

    changed glycine 12 to cysteine

    Promoter

    Available since

    July 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. AAV8-hSyn-flex-miR30-eGFP-shCrh

    Plasmid

    #132715

    Purpose

    Encodes Cre-dependent short hairpin RNA (shRNA) that targets the 3’-untranslated region of the rat Crh gene which encodes corticotropin releasing factor

    Depositor

    Robert Messing

    Article

    Pomrenze et al Cell Rep. 2019 Oct 1;29(1):13-21.e4. doi

    Insert

    GFP-shCrh (Crh Rat)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Synapsin 1

    Available since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. 8047_GFP-Kras2B_ires_puro

    Plasmid

    #64371

    Purpose

    This a retroviral expression plasmid expressing GFP tagged wild type Kras 2B oncogene along with puro resistance gene

    Depositor

    Georgios Stathopoulos

    Article

    Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

    Insert

    mouse Kras2B (Kras Mouse)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mutation

    none

    Promoter

    Available since

    July 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMX-53BP1(1-1710) 3LA

    Plasmid

    #185698

    Purpose

    Mammalian Expression, Retroviral vector that encodes a 3LA mutant of 53BP1 that disrupts three RIF1-binding motifs and abolishes the 53BP1-dependent recruitment of RIF1 to DNA double-strand breaks.

    Depositor

    Daniel Durocher

    Article

    Setiaputra et al Mol Cell. 2022 Apr 7;82(7):1359-1371.e9.

    Insert

    53BP1(1-1710) 3LA (TP53BP1 Human)
    

    Use

    Retroviral

    Tags

    Flag tag, HA tag, and tetracysteine tag

    Expression

    Mammalian

    Mutation

    L175A, L517A, and L693A

    Promoter

    SV40

    Available since

    July 7, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pMX-53BP1(1-1710) 3LA7A

    Plasmid

    #185707

    Purpose

    Mammalian Expression, Retroviral vector that encodes 7A and 3LA mutant of 53BP1. Prevents shieldin and RIF1 recruitment to sites of double-strand breaks and disrupts 53BP1 activity.

    Depositor

    Daniel Durocher

    Article

    Setiaputra et al Mol Cell. 2022 Apr 7;82(7):1359-1371.e9.

    Insert

    53BP1(1-1710) 3LA7A (TP53BP1 Human)
    

    Use

    Retroviral

    Tags

    Flag tag, HA tag, and tetracysteine tag

    Expression

    Mammalian

    Mutation

    T302A, S452A, S523A, S543A, S625A, S784A, and S89…

    Promoter

    SV40

    Available since

    July 1, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pcDNA3-sACE2v2(732)-IgG1

    Plasmid

    #154105

    Purpose

    Mammalian expression plasmid for dimeric soluble ACE2 high affinity variant 2 (IgG1-Fc fusion)

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Use

    Tags

    Fc region of human IgG1

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease and neck domains …

    Promoter

    CMV

    Available since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-sACE2v2(732)-8h

    Plasmid

    #154102

    Purpose

    Mammalian expression plasmid for dimeric soluble ACE2 high affinity variant 2 (8his tag)

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Use

    Tags

    Short GSG linker and 8his tag

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease domain (a.a. M1-D…

    Promoter

    CMV

    Available since

    June 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge