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Lenti-sgCdkn2a#2/Cre

Plasmid

#173630

Purpose

Expresses a Cdkn2a-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Cdkn2a (Cdkn2a Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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pOEM1_pCMV:bmp4-pPH:vsvged

Plasmid

#111157

Purpose

Baculovirus rescue vector, VSVGED pseudotype, hShh

Depositor

Elly Tanaka

Article

Wagner et al Dev Cell. 2017 Mar 27;40(6):608-617.e6.

Insert

hBmp4 (BMP4 Human)

Use

Baculoviral rescue shuttle vector

Expression

Insect and Mammalian

Mutation

wt

Promoter

CMV

Available Since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3-Flag-SPRTN-Y117C

Plasmid

#110216

Purpose

Expression in mammalian cells of of full-length SPRTN protein carrying Y117C mutation, a catalytically-impaired mutant, and Flag-tag at N-terminus.

Depositor

Ivana Marinovic-Terzic, Kristijan Ramadan

Article

Vaz et al Mol Cell. 2016 Nov 17;64(4):704-719. doi

Insert

SPRTN (SPRTN Human)

Tags

Flag

Expression

Mammalian

Mutation

Y117C, P296L (see depositor comments below)

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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Lenti-sgKeap1#2/Cre

Plasmid

#173640

Purpose

Expresses a Keap1-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Keap1 (Keap1 Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgDnmt3a#1/Cre

Plasmid

#173587

Purpose

Expresses a Dnmt3a-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Dnmt3a (Dnmt3a Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003 LIAS-1

Plasmid

#184478

Purpose

Lentivirus gRNA targeting human LIAS gene

Depositor

Todd Golub, Peter Tsvetkov

Article

Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

Insert

LIAS (LIAS Human)

Use

Lentiviral

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD003 LIAS-2

Plasmid

#184479

Purpose

Lentivirus gRNA targeting human LIAS gene

Depositor

Todd Golub, Peter Tsvetkov

Article

Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

Insert

LIAS (LIAS Human)

Use

Lentiviral

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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Bxb1Donor_G102H_ADRB2_LucReporter

Plasmid

#129794

Purpose

encodes a donor vector for recombination with the Bxb1 recombinase with variant G102H of the Beta-2 Adrenergic Receptor and a cAMP-responsive luciferase reporter gene

Depositor

Sriram Kosuri

Article

Jones et al Elife. 2020 Oct 21;9. pii: 54895. doi: 1

Insert

ADRB2 (ADRB2 Human)

Use

Synthetic Biology

Tags

3x FLAG

Expression

Mammalian

Mutation

G102H

Promoter

N/A

Available Since

Sept. 17, 2021

Availability

Academic Institutions and Nonprofits only

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KHBD00474

Plasmid

#39584

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG17077 (pnt Fly)

Use

Gateway Cloning

Promoter

none

Available Since

Aug. 20, 2012

Availability

Academic Institutions and Nonprofits only
LCV2_AAVS1_sgRNA_3

Plasmid

#155089

Purpose

lentiviral plasmid expressing Cas9 and gRNA targeting AAVS1 safe harbor locus

Depositor

Jason Moffat

Article

Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

Insert

AAVS1_sgRNA_3

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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R620-M21-303: CMV51p> SARS-CoV-2 S(1-1213)-2P-T4f-His6 B.1.429

Plasmid

#170200

Purpose

Mammalian expression of SARS-CoV-2 soluble spike trimer protein (B.1.429 variant)

Depositor

Dominic Esposito

Article

Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

Insert

SARS-CoV-2 S (spike) B.1.429 (S SARS-CoV-2)

Tags

His6

Expression

Mammalian

Mutation

S13I, W152C, L452R, D614G

Promoter

CMV51

Available Since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pNIC-ZB-SPRTN-Y117C-FL

Plasmid

#110219

Purpose

Expression in E. coli of full-length SPRTN protein carrying Y112A mutation, a catalytically impaired mutant, with His and ZB tags at N-terminus.

Depositor

Kristijan Ramadan

Article

Vaz et al Mol Cell. 2016 Nov 17;64(4):704-719. doi

Insert

SPRTN (SPRTN Human)

Tags

His6 and ZB

Expression

Bacterial

Mutation

Y117C, P296L (see depositor comments below)

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-CARMA2sh-E142G

Plasmid

#97070

Purpose

Expresses Psoriasis-Linked mutant CARD14/CARMA2 in mammalian cells

Depositor

Pasquale Vito

Article

Scudiero et al Cell Death Dis. 2017 Feb 23;8(2):e2627.

Insert

CARD14 isoform 3 (CARD14 Human)

Tags

HA

Expression

Mammalian

Mutation

changed Glutamic Acid 142 to Glycine

Promoter

CMV

Available Since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pHR-HaloTag-P2A-RSV M2-1-C-terminal-1xSunTag

Plasmid

#246867

Purpose

Express HaloTag-P2A-RSV M2-1-C-terminal-1xSunTag (M2-1exo-SunTag) for RSV imaging.

Depositor

Marvin Tanenbaum

Article

Ratnayake et al Nature. 2026 Jan 28. doi: 10.1038/s41586

Insert

HaloTag-P2A-RSV M2-1-C-terminal-1xSunTag

Use

Lentiviral

Tags

HaloTag

Expression

Mammalian

Mutation

The RSV M2-1 protein with a C-terminal insertion …

Promoter

SFFV

Available Since

Jan. 29, 2026

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-SUMO-m-cGAS(I309T)

Plasmid

#232295

Purpose

Expression of mutant mouse cGAS protein (I309T)

Depositor

Thomas Tuschl

Article

Skeldon et al Commun Chem. 2025 Mar 22;8(1):88. doi: 1

Insert

MB21D1 (Cgas Mouse)

Expression

Bacterial

Mutation

I309T

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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BPK1520-sgRNA GLB1

Plasmid

#184378

Purpose

Expresses a sgRNA to edit GLB1 gene NM_000404.2_c.907A>G

Depositor

David Gilot

Article

Leclerc et al CRISPR J. 2023 Feb;6(1):17-31. doi: 10.1

Insert

sgRNA targeting GLB1 (GLB1 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-EA-evoAPOBEC1max_NG-BlastR

Plasmid

#232721

Purpose

C-to-T base editor

Depositor

David Liu

Article

Matuszek et al Nat Genet. 2025 May 26. doi: 10.1038/s41

Insert

evoAPOBEC1max H47E+S48A-nCas9(NG)-UGI-UGI

Expression

Mammalian

Mutation

within evoAPOBEC1: H47E+S48A; within Cas9 D10A

Available Since

March 3, 2025

Availability

Academic Institutions and Nonprofits only

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pICH86966::AtU6p::sgRNA:NbVPE1a/b

Plasmid

#223218

Purpose

Expresses an sgRNA targeting the NbVPE1a/b genes in Nicotiana benthamiana with an Arabidopsis U6 promoter

Depositor

Tsepo Tsekoa

Article

Singh et al Front Plant Sci. 2022 Aug 18;13:953654.

Insert

NbVPE1a/b

Use

CRISPR and Synthetic Biology

Tags

None

Expression

Plant

Promoter

Arabidopsis U6 promoter

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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TH1274-054_Jie_EWSR1_pOCC177_pOEM1-N-HIS-MBP-PS-NotI-AscI-TEV-SNAP

Plasmid

#221896

Purpose

Shuttle vector for baculovirus production, using FlashBac bacmid

Depositor

Anthony Hyman

Article

Wang et al Cell. 2018 Jul 26;174(3):688-699.e16. do

Insert

EWSR1 (EWSR1 Human)

Tags

6xHis-MBP-PreScission and TEV-SNAP-tag

Expression

Insect

Mutation

wt

Promoter

PH promoter

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-3xFLAG-TAF5-∆ex8

Plasmid

#209050

Purpose

Vector for generating Flp-In cell lines allowing dox-inducible mammalian expression of 3xFlag-tagged TAF5 isoform lacking the alternative exon-8 (∆ex8)

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Insert

TAF5 deltaexon-8

Tags

FLAG

Expression

Mammalian

Promoter

CMV/TO inducible promoter

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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